AB253843

Human Anti-Ubiquitin+1/UBB+1 Antibody Pair - BSA and Azide free

RabMAb
Recombinant
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Human Anti-Ubiquitin+1/UBB+1 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human Anti-Ubiquitin+1/UBB+1.

View Alternative Names

Polyubiquitin-B, UBB

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Sandwich ELISA - Human Anti-Ubiquitin+1/UBB+1 Antibody Pair - BSA and Azide free (AB253843)

sELISA

Lab

Sandwich ELISA - Human Anti-Ubiquitin+1/UBB+1 Antibody Pair - BSA and Azide free (AB253843)

Sandwich ELISA of ab253843 with the capture antibody ab285010 dilution at 2 µg/mL and detector antibody ab285116 dilution at 0.5 µg/mL.

Key facts

Reacts with

Human

Assay type

ELISA set

Range

31.25 - 2000 pg/mL

Assay Platform

Reagents

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Reactivity data

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Product details

Human Ubiquitin+1 is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify Human Ubiquitin+1 in sandwich ELISAs and many other pair-based applications.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The pair can be used in variety of assays and platforms including but not limited to:

Sandwich ELISA
FRET/TR-FRET/HTRF
Meso Scale Discovery^® (MSD^®)
Bead-based assays
AlphaLISA^®/AlphaScreen^®
DELFIA^® immunoassays
Simoa^® and Single Molecule Counting (SMC™) immunoassays
Multiplex

Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:

Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
Concentration of ~1 mg/ml as measured by the protein A280 method.

We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.

Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.

Please note:

The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.

The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.

We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.

Antibody properties:

Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg

Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg

Concentration: ~1 mg/ml

Storage buffer: 100% PBS

Form: Liquid

Isotype: IgG

Recombinant monoclonal antibodies offer several advantages including:

- High batch-to-batch consistency and reproducibility

- Improved sensitivity and specificity

- Long-term security of supply

- Animal-free production

Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.

AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.

Simoa is a registered trademark of Quanterix, Inc.

SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ubiquitin+1 (UBB+1) is an altered form of the ubiquitin protein which plays a significant role in protein degradation. This mutant ubiquitin results from a molecular error during transcription known as a frameshift mutation. The mass of UBB+1 is slightly greater than that of the normal ubiquitin due to an extra amino acid. UBB+1 is expressed primarily in neuronal cells where it accumulates due to its impaired function in tagging proteins for degradation by the proteasome.

Biological function summary

The alteration in UBB+1 affects the cellular protein quality control system. UBB+1 cannot be recognized properly for degradation itself and hinders the ubiquitin-proteasome system (UPS) by competing with normal ubiquitin. UBB+1 does not just influence single proteins; it might also intervene in larger complexes related to proteostasis. Its aberrant accumulation disrupts the clearance of damaged or misfolded proteins which can impact cellular health.

Pathways

Ubiquitin+1 affects several fundamental cellular processes by disturbing the UPS pathway. UBB+1 interacts with proteins involved in the ubiquitin-proteasome pathway such as E1 activating enzymes E2 conjugating enzymes and E3 ligases. Through these associations UBB+1 can induce stress in protein turnover and impair cellular regulation with direct implications in the processes related to protein homeostasis. The effect on these pathways can lead to wider disruptions in cellular signaling and maintenance.

Ubiquitin+1 has a notable impact on neurodegenerative diseases particularly Alzheimer's disease. It is associated with the accumulation of neurofibrillary tangles and plaques seen in these conditions. UBB+1 also connects with proteins like amyloid-beta which play central roles in Alzheimer's pathology. Furthermore UBB+1 accumulation has implications in Huntington’s disease where it links with the aggregation-prone mutant huntingtin protein. These associations highlight the significance of UBB+1 in disease mechanisms involving protein aggregation and cellular stress responses.

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

Ubiquitin. Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked : Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in proteotoxic stress response and cell cycle; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.

See full target information UBB

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Anti-Ubiquitin+1/UBB+1 Antibody Pair - BSA and Azide free

Sandwich ELISA - Human Anti-Ubiquitin+1/UBB+1 Antibody Pair - BSA and Azide free (AB253843)

Key facts

Reacts with

Assay type

Range

Assay Platform

Reactivity data

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise