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Human VEGF R1 (FLT1) Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human VEGF R1 (FLT1).

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Images

Sandwich ELISA - Human VEGF R1 (FLT1) Antibody Pair - BSA and Azide free (AB253794), expandable thumbnail

Key facts

Assay type

ELISA set

Reactive species

Human

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info
-
Notes

-

Target data

Function

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. Acts as a positive regulator of postnatal retinal hyaloid vessel regression (By similarity). May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. Promotes PGF-mediated proliferation of endothelial cells, proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts (in vitro). Has very high affinity for VEGFA and relatively low protein kinase activity; may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR. Modulates KDR signaling by forming heterodimers with KDR. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leading to activation of phosphatidylinositol kinase and the downstream signaling pathway. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Phosphorylates SRC and YES1, and may also phosphorylate CBL. Promotes phosphorylation of AKT1 at 'Ser-473'. Promotes phosphorylation of PTK2/FAK1 (PubMed:16685275).Isoform 1Phosphorylates PLCG.Isoform 2May function as decoy receptor for VEGFA.Isoform 3May function as decoy receptor for VEGFA.Isoform 4May function as decoy receptor for VEGFA.Isoform 7Has a truncated kinase domain; it increases phosphorylation of SRC at 'Tyr-418' by unknown means and promotes tumor cell invasion.

Alternative names

What's included?

10 x 96 Tests
Components
Human FLT1 Capture Antibody (unconjugated)
1 x 100 µg
Human FLT1 Detector Antibody (unconjugated)
1 x 100 µg

Recommended products

Human VEGF R1 (FLT1) Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human VEGF R1 (FLT1).

Key facts

Assay type

ELISA set

Reactive species

Human

Assay Platform

Reagents

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

  • Human VEGF R1 (FLT1) is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify Human VEGF R1 (FLT1) in sandwich ELISAs and many other pair-based applications.
  • The pair can be used in variety of assays and platforms including but not limited to:
  • - Sandwich ELISA
  • - FRET/TR-FRET/HTR
  • - Meso Scale Discovery® (MSD®)
  • - Luminex® and bead-based assays
  • - AlphaLISA®/AlphaScreen®
  • - DELFIA® immunoassays
  • - Simoa® and Single Molecule Counting (SMC™) immunoassays
  • - Multiplex

  • Our antibody pairs are supplied in a a carrier-free format that is conjugation-ready:
  • - Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
  • - Concentration of ~1 mg/ml as measured by the protein A280 method.

  • Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for biotin, HRP, gold, and fluorescent dyes.
  • We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation service

  • Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.

  • Please note:
  • The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.
  • The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.
  • We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.

  • Antibody properties:
  • Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 μg
  • Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 μg
  • Concentration: ~1 mg/ml
  • Storage buffer: 100% PBS
  • Form: Liquid
  • Isotype: IgG

  • Recombinant monoclonal antibodies offer several advantages including:
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free production

  • Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
  • Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.
  • Luminex is a trademark of Luminex Corporation, registered in the US and other countries.
  • AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.
  • Simoa is a registered trademark of Quanterix, Inc.
  • SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The pair can be used in variety of assays and platforms including but not limited to:

  • Sandwich ELISA
  • FRET/TR-FRET/HTRF
  • Meso Scale Discovery® (MSD®)
  • Bead-based assays
  • AlphaLISA®/AlphaScreen®
  • DELFIA® immunoassays
  • Simoa® and Single Molecule Counting (SMC™) immunoassays
  • Multiplex

Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:

  • Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
  • Concentration of ~1 mg/ml as measured by the protein A280 method.

We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.

Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.

Please note:

The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.

The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.

We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.

Antibody properties:

Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg

Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg

Concentration: ~1 mg/ml

Storage buffer: 100% PBS

Form: Liquid

Isotype: IgG

Recombinant monoclonal antibodies offer several advantages including:

- High batch-to-batch consistency and reproducibility

- Improved sensitivity and specificity

- Long-term security of supply

- Animal-free production

Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.

AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.

Simoa is a registered trademark of Quanterix, Inc.

SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

VEGF Receptor 1 also known as FLT-1 is a protein that plays a critical role in the regulation of angiogenesis. Its mass is approximately 180 kDa. FLT-1 is a receptor tyrosine kinase expressed mainly on the surface of endothelial cells but also in monocytes and macrophages. The receptor binds vascular endothelial growth factor (VEGF) and placental growth factor (PlGF) which initiates a series of signaling cascades important for vascular development and function.

Biological function summary

FLT-1 mediates important cellular responses such as cell migration and proliferation. Although it often functions in conjunction with other receptors like VEGFR-2 FLT-1 has unique binding affinities that modulate the availability of VEGF for other receptors. FLT-1 proteins as part of complex signaling networks actively contribute to maintaining vascular homeostasis and regulating blood vessel permeability.

Pathways

FLT-1 is an important component in the VEGF signaling pathway and also part of the PlGF/VEGF pathway. The receptor serves as a decoy to regulate the amount of VEGF interaction with VEGFR-2 influencing endothelial cell function and vascular permeability. By sequestering VEGF FLT-1 helps maintain the balance in this angiogenic signaling network interacting with proteins like VEGF PlGF and the anti-VEGF agents that target these pathways.

Associated diseases and disorders

FLT-1 is implicated in cancer and diabetic retinopathy. Overexpression or dysregulation of FLT-1 can contribute to tumor angiogenesis which can enhance tumor growth and metastasis. In diabetic retinopathy abnormal FLT-1 activity affects retinal blood vessels leading to vision impairment. Similar interactions involve VEGFR-2 and other downstream effectors emphasizing the potential of FLT-1 as a therapeutic target for conditions associated with excessive angiogenesis and vascular dysfunction.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Human VEGF R1 (FLT1) Antibody Pair - BSA and Azide free (ab253794), expandable thumbnail

    Sandwich ELISA - Human VEGF R1 (FLT1) Antibody Pair - BSA and Azide free (ab253794)

    Example of Human VEGF R1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com