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AB312860

Human VEGFA Antibody Pair - BSA and Azide free

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Human VEGFA Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human VEGFA.

View Alternative Names

VEGF, VEGFA, L-VEGF, Vascular permeability factor, VPF

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Sandwich ELISA - Human VEGFA Antibody Pair - BSA and Azide free (AB312860)
  • sELISA

Supplier Data

Sandwich ELISA - Human VEGFA Antibody Pair - BSA and Azide free (AB312860)

Sandwich ELISA of ab312860 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

Key facts

Reacts with

Human

Assay type

ELISA set

Range

7.8 - 500 pg/mL

Assay Platform

Reagents

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human VEGFA Antibody Pair - BSA and Azide free (ab312860) is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify Human VEGFA in sandwich ELISAs and many other pair-based applications.
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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab312860-10x96Tests", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human VEGFA Capture Antibody (unconjugated)", "number":"AB312860-CMP01", "productcode":"AB312861" }, { "size":"1 x 100 µg", "name":"Human VEGFA Detector Antibody (unconjugated)", "number":"AB312860-CMP02", "productcode":"AB312862" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VEGF or Vascular Endothelial Growth Factor is a signaling protein that plays an essential role in blood vessel formation. Known also as VEGFA its molecular mass is approximately 45 kDa. VEGF is expressed in a variety of tissues especially those with high angiogenic activity such as endothelial cells macrophages and platelets. This protein activates receptors on the surface of endothelial cells triggering a cascade of intracellular events that lead to cellular proliferation migration and increased vascular permeability.
Biological function summary

This protein regulates angiogenesis the process through which new blood vessels form from pre-existing vasculature. It interacts with specific tyrosine kinase receptors such as VEGFR-1 and VEGFR-2. VEGF is not part of a multi-protein complex but it can form homodimers which significantly enhances its activity. It ensures proper vascularization during development and wound healing by directing the growth and branching of vessels. This makes it an important factor in supplying oxygen and nutrients to tissues.

Pathways

VEGF is primarily involved in the VEGF signaling and PI3K/AKT pathways. These pathways allow it to mediate endothelial cell function and survival. In the VEGF signaling pathway it associates closely with members like VEGFR-2 and PLCγ1 which activate further signal transduction needed for angiogenesis. The PI3K/AKT pathway linked with cell proliferation and survival also interacts with VEGF connecting it to other important proteins like AKT and mTOR highlighting its significant role in cellular responses to environmental stimuli.

VEGF has a strong association with cancer and age-related macular degeneration. In cancer overexpression of VEGF leads to pathological angiogenesis providing tumors with the blood supply needed for growth and metastasis. Tumor cells through hypoxia-inducible factors (HIF) upregulate VEGF expression correlating with cancer progression. In age-related macular degeneration abnormal blood vessel growth influenced by VEGF contributes to visual impairment and blindness further implicating the protein in ocular diseases. Targeted therapies often aim at inhibiting VEGF activity to control the disease progression.
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Product protocols

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Target data

N-VEGF. Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed : 35455969). Involved in protecting cells from hypoxia-mediated cell death (By similarity).. VEGFA. Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth (PubMed : 34530889). Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Also binds the DEAR/FBXW7-AS1 receptor (PubMed : 17446437).. Isoform VEGF165B. Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
See full target information VEGFA
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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