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AB219520

Mouse Angiopoietin 2 Matched Antibody Pair Kit

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Mouse Angiopoietin 2 Matched Antibody Pair Kit is a kit for ELISA development for the measurement of Mouse Angiopoietin 2 by sandwich ELISA method.

View Alternative Names

Agpt2, Angpt2, Angiopoietin-2, ANG-2

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ELISA - Mouse Angiopoietin 2 Matched Antibody Pair Kit (AB219520)
  • ELISA

Supplier Data

ELISA - Mouse Angiopoietin 2 Matched Antibody Pair Kit (AB219520)

Mouse Angiopoietin 2 standard curve.

Standard calibration curve. Background subtracted values are graphed.

Key facts

Detection method

Colorimetric

Reacts with

Mouse

Assay type

ELISA set

Sensitivity

= 4.7 pg/mL

Range

62.5 - 4000 pg/mL

Assay Platform

Reagents

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Complementary Products

Primary Antibodies

AB155106

Anti-Angiopoietin 2/ANG2 antibody [EPR2891(2)]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR2891(2)] (AB155106)

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Assay Kits

AB209883

Mouse Angiopoietin 2 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse Angiopoietin 2 ELISA Kit (AB209883)

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Assay Kits

AB210895

Mouse IL-1 beta Matched Antibody Pair Kit

Recombinant

Sandwich ELISA - Mouse IL-1 beta Matched Antibody Pair Kit (AB210895)

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Assay Kits

AB210890

Mouse Albumin Matched Antibody Pair Kit

Recombinant

Sandwich ELISA - Mouse Albumin Matched Antibody Pair Kit (AB210890)

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Reactivity data

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Product details

Mouse Angiopoietin 2 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse Angiopoietin 2.Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our . An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab209883. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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What's included?

{ "values": { "5x96Tests": { "sellingSize": "5 x 96 Tests", "publicAssetCode":"ab219520-5x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Mouse Angiopoetin 2 Lyophilized Protein", "number":"AB219520-CMP03", "productcode":"" }, { "size":"1 x 12.5 µg", "name":"Mouse Angiopoetin 2 Detector Antibody", "number":"AB219520-CMP02", "productcode":"" }, { "size":"1 x 50 µg", "name":"Mouse Angiopoetin 2 Capture Antibody", "number":"AB219520-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Angiopoietin-2 also known as ANG2 or ANGPT2 is a glycoprotein with a molecular mass of approximately 70 kDa. It plays an important role in angiogenesis facilitating blood vessel remodeling and maturation. The protein is mainly expressed in endothelial cells where it functions by binding to the Tie2 receptor partially disrupting the Angiopoietin-Tie2 signaling axis. This disruption modulates vascular stability and permeability. The lyophilized form of the ANG-2 protein is often used in research for functional assays like ANG-2 ELISA to quantify its levels in various biological samples.
Biological function summary

Human angiogenesis processes are significantly affected by Angiopoietin-2 which serves as an antagonist to Angiopoietin-1 in regulating vascular dynamics. Although it does not form a stable complex by itself ANG2 activity is closely linked to its interactions with the Tie2 receptor modulating signal pathways that govern endothelial cell survival and permeability. Because of its dual role either stabilizing or destabilizing blood vessels under different conditions ANG2 serves as an important regulator during both normal physiological events and pathophysiological conditions.

Pathways

Angiopoietin-2 significantly contributes to the angiogenesis signaling network. It is deeply involved in the VEGF (Vascular Endothelial Growth Factor) pathway where ANG2 acts to balance VEGF activities in angiogenesis and vessel permeability. The interplay between Angiopoietin-2 and other proteins such as Angiopoietin-1 which stabilizes the vessels is vital for the precise regulation of blood vessel homeostasis during normal and disturbed conditions.

Angiopoietin-2 has a prominent role in the pathology of diseases like cancer and diabetic retinopathy. In cancer excessive ANG2 expression may lead to increased tumor angiogenesis and metastasis making it a target for cancer therapies. In diabetic retinopathy elevated levels of ANG2 correlate with vascular leakage contributing to disease progression. The relationship between Angiopoietin-1 and Angiopoietin-2 in these conditions emphasizes their collective impact on vascular anomalies associated with these disorders.
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Product protocols

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Target data

Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling (By similarity). Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1 (By similarity). In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression (By similarity). In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal (By similarity). Involved in the regulation of lymphangiogenesis (PubMed : 28179430, PubMed : 32908006).
See full target information Angpt2
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Product promise

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