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AB221440

Mouse Apolipoprotein AI Matched Antibody Pair Kit

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(1 Publication)

Mouse Apolipoprotein AI Matched Antibody Pair Kit is a kit for ELISA development for the measurement of Mouse Apolipoprotein AI by sandwich ELISA method.

View Alternative Names

Apolipoprotein A-I, Apo-AI, ApoA-I, Apolipoprotein A1, Apoa1

1 Images
ELISA - Mouse Apolipoprotein AI Matched Antibody Pair Kit (AB221440)
  • ELISA

Supplier Data

ELISA - Mouse Apolipoprotein AI Matched Antibody Pair Kit (AB221440)

Mouse Apolipoprotein AI standard curve.

Standard calibration curve. Background substracted values are graphed.

Key facts

Detection method

Colorimetric

Reacts with

Mouse

Assay type

ELISA set

Sensitivity

= 40.3 pg/mL

Range

156.2 - 10000 pg/mL

Assay Platform

Reagents

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Complementary Products

Reagents, Controls & Accessories

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Primary Antibodies

AB52945

Anti-Apolipoprotein A I antibody [EP1368Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Apolipoprotein A I antibody [EP1368Y] (AB52945)

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AB246841

Human Albumin Matched Antibody Pair Kit

Recombinant

ELISA - Human Albumin Matched Antibody Pair Kit (AB246841)

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Reactivity data

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Product details

Human Apolipoprotein AI Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human Apolipoprotein AI.Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our . An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab238260. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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What's included?

{ "values": { "5x96Tests": { "sellingSize": "5 x 96 Tests", "publicAssetCode":"ab221440-5x96Tests", "assetComponentDetails": [ { "size":"1 x 12.5 µg", "name":"Mouse Apolipoprotein AI Detector Antibody", "number":"AB221440-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Mouse Apolipoprotein AI Lyophilized Protein", "number":"AB221440-CMP03", "productcode":"" }, { "size":"1 x 50 µg", "name":"Mouse Apolipoprotein AI Capture Antibody", "number":"AB221440-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein A-I (ApoA-I) is a major component of high-density lipoprotein (HDL) particles often referred to as 'good cholesterol'. It has a molecular mass of approximately 28 kDa. ApoA-I is mainly expressed in the liver and intestine. It plays a mechanical role in the reverse transport of cholesterol acting to facilitate the efflux of cholesterol from tissues to the liver for excretion. Its alternative names include ApoAI ApoA1 and a component of the AI kits used for measuring this protein.
Biological function summary

ApoA-I functions in cholesterol homeostasis and inflammation. It is a structural component of the HDL complex that mobilizes cholesterol. ApoA-I acts as an activator of the enzyme lecithin-cholesterol acyltransferase (LCAT) which is essential for the maturation of HDL particles. This maturation is necessary for effective cholesterol transport and clearance. ApoA-I's ability to stabilize HDL particles and enhance their functionality makes it significant for maintaining lipid balance and cellular homeostasis.

Pathways

The interaction of ApoA-I with HDL formation and function marks its role in lipid metabolism pathways. Its participation in the reverse cholesterol transport pathway highlights its influence on cardiovascular health. ApoA-I also interacts with other proteins like ApoA-II and paraoxonase-1 which further influence lipid metabolism and antioxidant activities. Understanding these relationships helps elucidate the dynamics of cholesterol removal from the bloodstream.

Disturbances in ApoA-I levels correlate with cardiovascular disease and atherosclerosis. Deficiency or dysfunction in ApoA-I can impair HDL function leading to poor cholesterol removal and buildup within arteries. It is also connected to amyloidosis where misfolded ApoA-I forms deposits in tissues. Understanding these pathological conditions helps researchers target ApoA-I in therapeutic strategies to mitigate disease progression often studying it alongside proteins like ApoB which is associated with low-density lipoprotein (LDL) particles.
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Product protocols

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Target data

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
See full target information Apoa1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of lipid research 63:100153 PubMed34808192

2021

Oxidized phospholipids cause changes in jejunum mucus that induce dysbiosis and systemic inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Pallavi Mukherjee,Arnab Chattopadhyay,Victor Grijalva,Nasrin Dorreh,Venu Lagishetty,Jonathan P Jacobs,Bethan L Clifford,Thomas Vallim,Julia J Mack,Mohamad Navab,Srinivasa T Reddy,Alan M Fogelman
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Product promise

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