Mouse Betacellulin Antibody Pair - BSA and Azide free (BTC) is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Mouse Betacellulin (BTC).
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Key facts
- Assay type
- ELISA set
- Reactive species
- Mouse
- Range
- 4.69 - 300 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.
Alternative names
Bcn, Btc, Probetacellulin
What's included?
Recommended products
Mouse Betacellulin Antibody Pair - BSA and Azide free (BTC) is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Mouse Betacellulin (BTC).
Key facts
- Assay type
- ELISA set
- Reactive species
- Mouse
- Range
- 4.69 - 300 pg/mL
- Assay Platform
- Reagents
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
The Antibody Pair can be used to quantify Mouse Betacellulin (BTC). BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (Mouse Betacellulin ELISA Kit (BTC) ab255722), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The pair can be used in variety of assays and platforms including but not limited to:
- Sandwich ELISA
- FRET/TR-FRET/HTRF
- Meso Scale Discovery® (MSD®)
- Bead-based assays
- AlphaLISA®/AlphaScreen®
- DELFIA® immunoassays
- Simoa® and Single Molecule Counting (SMC™) immunoassays
- Multiplex
Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:
- Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
- Concentration of ~1 mg/ml as measured by the protein A280 method.
We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.
Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.
Please note:
The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.
The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.
We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.
Antibody properties:
Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg
Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg
Concentration: ~1 mg/ml
Storage buffer: 100% PBS
Form: Liquid
Isotype: IgG
Recombinant monoclonal antibodies offer several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free production
Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.
AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.
Simoa is a registered trademark of Quanterix, Inc.
SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The BTC protein also known as betacellulin is a member of the epidermal growth factor (EGF) family that plays an important role in cell signaling. It is a growth factor with a molecular weight of approximately 32 kDa. BTC is expressed in a range of tissues including the pancreas kidney and small intestine. This protein binds to and activates the EGF receptor (EGFR) stimulating various cellular processes. Researchers often use the BTC mouse and BTC chimeras to study its function and physiological roles due to their relevance to human biology.
- Biological function summary
BTC plays an important role in regulating cell proliferation especially in the pancreas where it influences beta-cell growth and regeneration. It operates as part of a signaling complex that interacts with other members of the EGF family impacting various cellular responses. Researchers employ experimental tools like BTC ELISA kits to quantify BTC levels in biological samples aiding in the exploration of its biological functions and mechanisms. BTC’s ability to modulate growth pathways makes it a significant molecule under investigation.
- Pathways
BTC participates in the EGF/EGFR signaling pathway which is essential for controlling cellular growth and differentiation. This pathway involves the interaction of BTC with other ligands such as EGF and transforming growth factor-alpha (TGF-α) altering downstream signaling events. BTC further contributes to the activation of the MAPK/ERK pathway a critical process for transmitting signals from the cell surface to the DNA in the nucleus. Through these pathways BTC impacts cellular activities and balances multiple biological functions.
- Associated diseases and disorders
BTC is linked to conditions such as diabetes and various cancers. In diabetes BTC is associated with pancreatic function particularly influencing insulin production through its role in beta-cell regulation. In cancer abnormal BTC expression or its interactions with EGFR signaling components can lead to uncontrolled cell proliferation. Researchers study BTC’s interactions with proteins like EGF and TGF-α to understand its involvement in these disorders as therapeutic targeting of this axis holds potential for treatment strategies.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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1 product image
Sandwich ELISA - Mouse Betacellulin Antibody Pair - BSA and Azide free (BTC) (ab256757)
Representative standard curve from corresponding SimpleStep ELISA® Kit (Mouse Betacellulin ELISA Kit (BTC) ab255722), which uses the same antibody pair. For additional information on the performance of pair and kit, refer to the corresponding kit datasheet. Due to differences in the formulation and format of the antibodies in this pair, they cannot be used as substitutes for the antibody components in our SimpleStep ELISA® Kits.
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