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Mouse CXCL16 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Mouse CXCL16.

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Images

Sandwich ELISA - Mouse CXCL16 Antibody Pair - BSA and Azide free (AB241699), expandable thumbnail
  • Sandwich ELISA - Mouse CXCL16 Antibody Pair - BSA and Azide free (AB241699), expandable thumbnail

Key facts

Assay type

ELISA set

Reactive species

Mouse

Range

15.6 - 1000 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

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Target data

Function

Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo. Also acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis.

Alternative names

What's included?

10 x 96 Tests
Components
Mouse CXCL16 Capture Antibody (unconjugated)
1 x 100 µg
Mouse CXCL16 Detector Antibody (unconjugated)
1 x 100 µg

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Mouse CXCL16 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Mouse CXCL16.

Alternative names

Key facts

Assay type

ELISA set

Reactive species

Mouse

Range

15.6 - 1000 pg/mL

Assay Platform

Reagents

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

The Antibody Pair can be used to quantify Mouse CXCL16. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.

For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (Mouse CXCL16 ELISA Kit ab197744), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.

Supplementary info

Activity summary

CXCL16 also known as SR-PSOX is a chemokine that functions primarily in cellular signaling. It weighs approximately 32 kDa and exists in both membrane-bound and soluble forms. Primarily found in the spleen liver lungs and kidneys CXCL16 gets highly expressed on the surface of antigen-presenting cells such as dendritic cells and macrophages. This expression enables CXCL16 to perform its role in cell migration and adhesion by binding to its receptor CXCR6.

Biological function summary

CXCL16 plays a critical role in the immune response by mediating leukocyte migration. It acts as a chemoattractant for T cells especially those expressing the CXCR6 receptor facilitating their movement to sites of inflammation or injury. CXCL16 is not known to be part of a larger protein complex but interacts independently to moderate immune functions. It also participates in scavenging oxidized low-density lipoproteins linking it to processes in atherogenesis.

Pathways

CXCL16 is an important player in the inflammatory and immune pathways. It is involved in the NF-kB signaling pathway which regulates inflammatory responses and the chemokine signaling pathway. Through these pathways CXCL16 closely interacts with other chemokines and cytokines such as CCR5 and IL-8 helping coordinate immune surveillance and inflammatory cascade.

Associated diseases and disorders

CXCL16 is heavily implicated in atherosclerosis and rheumatoid arthritis. In atherosclerosis CXCL16 contributes to the accumulation of macrophages and foam cell formation in plaques. This connection associates it with inflammatory molecules like TNF-alpha. In rheumatoid arthritis CXCL16 expression is increased in synovial tissues contributing to tissue damage and inflammation potentially linked through interactions with other inflammatory mediators such as IL-6.

Product promise

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2 product images

  • Sandwich ELISA - Mouse CXCL16 Antibody Pair - BSA and Azide free (ab241699), expandable thumbnail

    Sandwich ELISA - Mouse CXCL16 Antibody Pair - BSA and Azide free (ab241699)

    Representative standard curve from corresponding SimpleStep ELISA® Kit (Mouse CXCL16 ELISA Kit ab197744), which uses the same antibody pair. For additional information on the performance of pair and kit, refer to the corresponding kit datasheet. Due to differences in the formulation and format of the antibodies in this pair, they cannot be used as substitutes for the antibody components in our SimpleStep ELISA® Kits.

  • Sandwich ELISA - Mouse CXCL16 Antibody Pair - BSA and Azide free (ab241699), expandable thumbnail

    Sandwich ELISA - Mouse CXCL16 Antibody Pair - BSA and Azide free (ab241699)

    Representative standard curve from corresponding SimpleStep ELISA® Kit (Mouse CXCL16 ELISA Kit ab197744), which uses the same antibody pair. For additional information on the performance of pair and kit, refer to the corresponding kit datasheet. Due to differences in the formulation and format of the antibodies in this pair, they cannot be used as substitutes for the antibody components in our SimpleStep ELISA® Kits.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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