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Mouse DNMT1 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Mouse DNMT1.

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Key facts

Assay type

ELISA set

Reactive species

Mouse

Range

39.06 - 5000 pg/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (By similarity). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (By similarity). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (By similarity). Promotes tumor growth (By similarity).

Alternative names

What's included?

10 x 96 Tests
Components
Mouse DNMT1 Capture Antibody (unconjugated)
1 x 100 µg
Mouse DNMT1 Detector Antibody (unconjugated)
1 x 100 µg

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Mouse DNMT1 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Mouse DNMT1.

Alternative names

Key facts

Assay type

ELISA set

Reactive species

Mouse

Range

39.06 - 5000 pg/mL

Assay Platform

Reagents

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

  • Mouse DNMT1 Antibody Pair is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify Mouse DNMT1 in sandwich ELISAs and many other pair-based applications.
  • The pair can be used in variety of assays and platforms including but not limited to:
  • - Sandwich ELISA
  • - FRET/TR-FRET/HTR
  • - Meso Scale Discovery® (MSD®)
  • - Luminex® and bead-based assays
  • - AlphaLISA®/AlphaScreen®
  • - DELFIA® immunoassays
  • - Simoa® and Single Molecule Counting (SMC™) immunoassays
  • - Multiplex

  • Our antibody pairs are supplied in a a carrier-free format that is conjugation-ready:
  • - Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
  • - Concentration of ~1 mg/ml as measured by the protein A280 method.

  • Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for biotin, HRP, gold, and fluorescent dyes.
  • We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation service

  • Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.

  • Please note:
  • The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.
  • The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.
  • We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.

  • Antibody properties:
  • Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 μg
  • Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 μg
  • Concentration: ~1 mg/ml
  • Storage buffer: 100% PBS
  • Form: Liquid
  • Isotype: IgG

  • Recombinant monoclonal antibodies offer several advantages including:
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free production

  • Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
  • Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.
  • Luminex is a trademark of Luminex Corporation, registered in the US and other countries.
  • AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.
  • Simoa is a registered trademark of Quanterix, Inc.
  • SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Mouse DNMT1 Antibody Pair - BSA and Azide free (ab253685), expandable thumbnail

    Sandwich ELISA - Mouse DNMT1 Antibody Pair - BSA and Azide free (ab253685)

    Sandwich ELISA of ab253685 with the capture antibody ab281245 dilution at 2 µg/mL and detector antibody ab281095 dilution at 0.5 µg/mL.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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