Mouse IL-1 beta Matched Antibody Pair Kit is a kit for ELISA development for the measurement of Mouse IL-1 beta by sandwich ELISA method.
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- Immunity 54:2595-2610.e72021Candida albicans elicits protective allergic responses via platelet mediated T helper 2 and T helper 17 cell polarization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYifan Wu,Zhimin Zeng,Yubiao Guo,Lizhen Song,Jill E Weatherhead,Xinyan Huang,Yuying Zeng,Lynn Bimler,Cheng-Yen Chang,John M Knight,Christian Valladolid,Hua Sun,Miguel A Cruz,Bernhard Hube,Julian R Naglik,Amber U Luong,Farrah Kheradmand,David B CorryPubMed 34506733
- Nature communications 10:582019Microglia and amyloid precursor protein coordinate control of transient Candida cerebritis with memory deficits.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYifan Wu et. al.PubMed 30610193
Key facts
- Detection method
- Colorimetric
- Assay type
- ELISA set
- Reactive species
- Mouse
- Range
- 7.8 - 500 pg/mL
- Sensitivity
- = 1.7 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.
Alternative names
Interleukin-1 beta, IL-1 beta, Il1b
What's included?
Recommended products
Mouse IL-1 beta Matched Antibody Pair Kit is a kit for ELISA development for the measurement of Mouse IL-1 beta by sandwich ELISA method.
Key facts
- Detection method
- Colorimetric
- Assay type
- ELISA set
- Reactive species
- Mouse
- Range
- 7.8 - 500 pg/mL
- Assay Platform
- Reagents
- Sensitivity
- = 1.7 pg/mL
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Mouse IL-1 beta Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse IL-1 beta.Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our . An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ELISA Accessory Pack ab210905).For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit Mouse IL-1 beta ELISA Kit ab197742. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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1 product image
Sandwich ELISA - Mouse IL-1 beta Matched Antibody Pair Kit (ab210895)
Example of Mouse IL-1 beta standard curve in 1X Cell Extraction Buffer PTR using recombinant IL-1 beta RabMAb antibody and a recombinant monoclonal antibody. Background-subtracted values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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