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AB210895

Mouse IL-1 beta Matched Antibody Pair Kit

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(2 Publications)

Mouse IL-1 beta Matched Antibody Pair Kit is a kit for ELISA development for the measurement of Mouse IL-1 beta by sandwich ELISA method.

View Alternative Names

Interleukin-1 beta, IL-1 beta, Il1b

1 Images
Sandwich ELISA - Mouse IL-1 beta Matched Antibody Pair Kit (AB210895)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-1 beta Matched Antibody Pair Kit (AB210895)

Example of Mouse IL-1 beta standard curve in 1X Cell Extraction Buffer PTR using recombinant IL-1 beta RabMAb antibody and a recombinant monoclonal antibody. Background-subtracted values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Reacts with

Mouse

Assay type

ELISA set

Sensitivity

= 1.7 pg/mL

Range

7.8 - 500 pg/mL

Assay Platform

Reagents

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Complementary Products

Primary Antibodies

AB283818

Anti-IL-1 beta antibody [RM1009]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunocytochemistry - Anti-IL-1 beta antibody [RM1009] (AB283818)

  • 0
  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Assay Kits

AB178013

Human IL-6 ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human IL-6 ELISA Kit (AB178013)

  • 5
  • 2
Assay Kits

AB255729

Mouse IL-10 ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse IL-10 ELISA Kit (AB255729)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse IL-1 beta Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse IL-1 beta.Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our . An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab197742. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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What's included?

{ "values": { "5x96Tests": { "sellingSize": "5 x 96 Tests", "publicAssetCode":"ab210895-5x96Tests", "assetComponentDetails": [ { "size":"2 x 12.5 µg", "name":"Mouse IL-1b Detector Antibody", "number":"AB210895-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse IL-1b Lyophilized Protein", "number":"AB210895-CMP03", "productcode":"" }, { "size":"2 x 50 µg", "name":"Mouse IL-1b Capture Antibody", "number":"AB210895-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis : its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.
See full target information Il1b
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Immunity 54:2595-2610.e7 PubMed34506733

2021

Candida albicans elicits protective allergic responses via platelet mediated T helper 2 and T helper 17 cell polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Yifan Wu,Zhimin Zeng,Yubiao Guo,Lizhen Song,Jill E Weatherhead,Xinyan Huang,Yuying Zeng,Lynn Bimler,Cheng-Yen Chang,John M Knight,Christian Valladolid,Hua Sun,Miguel A Cruz,Bernhard Hube,Julian R Naglik,Amber U Luong,Farrah Kheradmand,David B Corry

Nature communications 10:58 PubMed30610193

2019

Microglia and amyloid precursor protein coordinate control of transient Candida cerebritis with memory deficits.

Applications

Unspecified application

Species

Unspecified reactive species

Yifan Wu,Shuqi Du,Jennifer L Johnson,Hui-Ying Tung,Cameron T Landers,Yuwei Liu,Brittany G Seman,Robert T Wheeler,Mauro Costa-Mattioli,Farrah Kheradmand,Hui Zheng,David B Corry
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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