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AB218808

Mouse IL-18 Matched Antibody Pair Kit

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(3 Publications)

Mouse IL-18 Matched Antibody Pair Kit is a kit for ELISA development for the measurement of Mouse IL-18 by sandwich ELISA method.

View Alternative Names

IGIF, IL1F4, IL18, Interleukin-18, IL-18, Iboctadekin, Interferon gamma-inducing factor, Interleukin-1 gamma, IFN-gamma-inducing factor, IL-1 gamma

1 Images
ELISA - Mouse IL-18 Matched Antibody Pair Kit (AB218808)
  • ELISA

Supplier Data

ELISA - Mouse IL-18 Matched Antibody Pair Kit (AB218808)

Mouse IL-18 standard curve.

Standard calibration curve. Background subtracted values are graphed.

Key facts

Detection method

Colorimetric

Reacts with

Mouse

Assay type

ELISA set

Sensitivity

= 15.1 pg/mL

Range

31.25 - 2000 pg/mL

Assay Platform

Reagents

Reactivity data

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Product details

Mouse IL-18 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse IL-18.Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our . An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab216165. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.

What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

Pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses (PubMed : 10653850). Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators (PubMed : 14528293, PubMed : 25500532, PubMed : 37993714). Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells (PubMed : 10653850). Involved in transduction of inflammation downstream of pyroptosis : its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed : 33883744).
See full target information IL18

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 9:e13753 PubMed36873506

2023

Mangiferin inhibits chronic stress-induced tumor growth in colorectal liver metastases via WAVE2 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yiming Tao,Xuefei Tian,Jia Luo,Hongyi Zhu,Yi Chu,Lei Pei

Nature communications 12:2674 PubMed33976226

2021

YAP promotes the activation of NLRP3 inflammasome via blocking K27-linked polyubiquitination of NLRP3.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Wang,Yening Zhang,Xueming Xu,Jianfeng Wu,Yue Peng,Jing Li,Ruiheng Luo,Lingmin Huang,Liping Liu,Songlin Yu,Ningjie Zhang,Ben Lu,Kai Zhao

Scientific reports 9:14534 PubMed31601922

2019

NSAIDs disrupt intestinal homeostasis by suppressing macroautophagy in intestinal epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ana M Chamoun-Emanuelli,Laura K Bryan,Noah D Cohen,Taylor L Tetrault,Joseph A Szule,Rola Barhoumi,Canaan M Whitfield-Cargile
View all publications
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