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AB275364

Mouse IP-10 Matched Antibody Pair Kit

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(1 Publication)

Mouse IP-10 Matched Antibody Pair Kit is a kit for ELISA development for the measurement of Mouse IP-10 by sandwich ELISA method.

View Alternative Names

Crg2, Ifi10, Inp10, Scyb10, Cxcl10, C-X-C motif chemokine 10, 10 kDa interferon gamma-induced protein, C7, Interferon-gamma induced protein CRG-2, Small-inducible cytokine B10, Gamma-IP10, IP-10

1 Images
Sandwich ELISA - Mouse IP-10 Matched Antibody Pair Kit (AB275364)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IP-10 Matched Antibody Pair Kit (AB275364)

Mouse IP-10 standard curve.

Standard calibration curve. Background subtracted values are graphed.

Key facts

Detection method

Colorimetric

Reacts with

Mouse

Assay type

ELISA set

Sensitivity

= 0.168 pg/mL

Range

7.8 - 500 pg/mL

Assay Platform

Reagents

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse IP-10 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse IP-10.Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our . An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab260067. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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What's included?

{ "values": { "5x96Tests": { "sellingSize": "5 x 96 Tests", "publicAssetCode":"ab275364-5x96Tests", "assetComponentDetails": [ { "size":"1 x 12.5 µg", "name":"Mouse IP-10 Detector Antibody", "number":"AB275364-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Mouse IP-10 Lyophilized Protein", "number":"AB275364-CMP03", "productcode":"" }, { "size":"1 x 50 µg", "name":"Mouse IP-10 Capture Antibody", "number":"AB275364-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IP10 also known as CXCL10 is a small cytokine belonging to the CXC chemokine family. It has a molecular mass of approximately 8.7 kDa. IP10 is secreted by several cell types such as monocytes endothelial cells and fibroblasts in response to interferon-gamma (IFN-γ). This protein is involved in immune responses and exhibits various roles especially in chemoattracting cells. Researchers often measure IP10 concentrations using ELISA kits such as the IP-10 ELISA to study its expression levels in different biological contexts.
Biological function summary

IP10 plays a role in modulating the activities of immune cells. It attracts T cells eosinophils monocytes and natural killer (NK) cells by binding to the CXCR3 receptor. IP10 is not part of a larger complex but interacts with other cytokines to influence cell migration and the immune response. High levels of IP10 can reflect strong immune activation which is why it is often measured in inflammatory conditions using standard assays like the IP-10 ELISA kits.

Pathways

The role of IP10 lies within the Th1-type immune response pathway. In this pathway IP10 works alongside other chemokines to recruit and activate immune cells to sites of inflammation or infection. It synergizes with IFN-γ to propagate immune signals. IP10 is also linked with the CXCR3 receptor which plays a critical role in these pathways providing a connection to other proteins such as CXCL9 and CXCL11 which have similar functions in cell-mediated immunity.

IP10 is associated with conditions like multiple sclerosis and rheumatoid arthritis. Elevated IP10 levels often correlate with disease activity in these disorders making it a potential biomarker for disease progression. The protein interacts with other inflammatory mediators such as TNF-α in regulating immune activity within these disease contexts. IP10's involvement in recruiting immune cells contributes to the pathogenic inflammation observed in these conditions.
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Product protocols

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Target data

Pro-inflammatory cytokine that is involved in a wide variety of processes such as chemotaxis, differentiation, and activation of peripheral immune cells, regulation of cell growth, apoptosis and modulation of angiostatic effects (By similarity) (PubMed : 28623423). Plays thereby an important role during viral infections by stimulating the activation and migration of immune cells to the infected sites (PubMed : 18624292, PubMed : 19017990, PubMed : 28468883). Mechanistically, binding of CXCL10 to the CXCR3 receptor activates G protein-mediated signaling and results in downstream activation of phospholipase C-dependent pathway, an increase in intracellular calcium production and actin reorganization. In turn, recruitment of activated Th1 lymphocytes occurs at sites of inflammation (By similarity). Activation of the CXCL10/CXCR3 axis also plays an important role in neurons in response to brain injury for activating microglia, the resident macrophage population of the central nervous system, and directing them to the lesion site. This recruitment is an essential element for neuronal reorganization (PubMed : 15456824).
See full target information Cxcl10
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oxidative medicine and cellular longevity 2022:4978556 PubMed35308172

2022

Suppressive Effect of Tetrahydrocurcumin on Lipopolysaccharide-Induced Inflammation by Suppressing JAK/STAT and Nrf2/HO-1 Pathways in Microglial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Wen Lin,Tzu-Chun Chen,Jui-Hsuan Yeh,Shang-Chun Tsou,Inga Wang,Ting-Jing Shen,Chen-Ju Chuang,Yuan-Yen Chang
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websiteProtocolBooklet
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Product promise

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