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AB215398

Mouse MIP1b Matched Antibody Pair Kit

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Mouse MIP1b Matched Antibody Pair Kit is a kit for ELISA development for the measurement of Mouse MIP1b by sandwich ELISA method.

View Alternative Names

Mip1b, Scya4, Ccl4, C-C motif chemokine 4, Immune activation protein 2, Macrophage inflammatory protein 1-beta, Protein H400, SIS-gamma, Small-inducible cytokine A4, ACT-2, ACT2, MIP-1-beta

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ELISA - Mouse MIP1b Matched Antibody Pair Kit (AB215398)
  • ELISA

Supplier Data

ELISA - Mouse MIP1b Matched Antibody Pair Kit (AB215398)

Mouse MIP1b standard curve.

Standard calibration curve. Background subtracted values are graphed.

Key facts

Detection method

Colorimetric

Reacts with

Mouse

Assay type

ELISA set

Sensitivity

= 0.67 pg/mL

Range

1.95 - 125 pg/mL

Assay Platform

Reagents

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Primary Antibodies

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Primary Antibodies

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AB210890

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Recombinant

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Reactivity data

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Product details

Mouse MIP1b Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse MIP1b.Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our . An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab215115. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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What's included?

{ "values": { "5x96Tests": { "sellingSize": "5 x 96 Tests", "publicAssetCode":"ab215398-5x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Mouse MIP1b Lyophilized Protein", "number":"AB215398-CMP03", "productcode":"" }, { "size":"1 x 12.5 µg", "name":"Mouse MIP1b Detector Antibody", "number":"AB215398-CMP02", "productcode":"" }, { "size":"1 x 50 µg", "name":"Mouse MIP1b Capture Antibody", "number":"AB215398-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCL4 also known as MIP-1 beta or MIP-1b has a mechanical role as a chemokine involved in immune response regulation. The protein has a mass of about 7.8 kDa. It expresses in various tissues including leukocytes where it mainly functions as a chemoattractant. MIP-1 beta helps recruit immune cells like natural killer cells and monocytes to sites of inflammation or injury. Researchers frequently measure its levels using assays like MIP-1 beta ELISA or CCL4 ELISA.
Biological function summary

CCL4 is critical in immune system modulation. It coordinates leukocyte activation and trafficking contributing to inflammation and immune surveillance. It does not function in isolation but rather interacts with other chemokines and receptors. CCL4 alongside its sibling chemokines forms a dynamic network that ensures a balanced immune response. It connects to receptors such as CCR5 playing substantial roles in its activities.

Pathways

CCL4 participates in essential signaling cascades that mediate immune responses. It is notably part of the chemokine signaling pathway which is instrumental in directing cell movement. CCL4 also engages with proteins like CCR5 in the inflammatory and immune response pathways. This interaction enhances communication between cells required for effective pathogen defense.

CCL4 plays roles in immune-related conditions. It is strongly linked to inflammatory diseases such as rheumatoid arthritis and HIV infection. In rheumatoid arthritis CCL4 levels are often elevated correlating with inflammation severity. During HIV infection CCL4 interacts with proteins like CCR5 a co-receptor the virus uses to enter host cells. This involvement makes MIP-1 beta a target for studies seeking therapeutic interventions in these diseases.
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Product protocols

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Target data

Monokine with inflammatory and chemokinetic properties.
See full target information Ccl4
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websiteProtocolBooklet
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Product promise

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