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AB216791

Mouse Pro-Collagen I alpha 1 Matched Antibody Pair Kit

4

(1 Review)

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(1 Publication)

Mouse Pro-Collagen I alpha 1 Matched Antibody Pair Kit is a kit for ELISA development for the measurement of Mouse Pro-Collagen I alpha 1 by sandwich ELISA method.

View Alternative Names

Cola1, Col1a1, Collagen alpha-1(I) chain, Alpha-1 type I collagen

1 Images
ELISA - Mouse Pro-Collagen I alpha 1 Matched Antibody Pair Kit (AB216791)
  • ELISA

Supplier Data

ELISA - Mouse Pro-Collagen I alpha 1 Matched Antibody Pair Kit (AB216791)

Mouse Pro-Collagen I alpha 1 standard curve.

Standard calibration curve. Background subtracted values are graphed.

Key facts

Detection method

Colorimetric

Reacts with

Mouse

Assay type

ELISA set

Sensitivity

= 2.25 pg/mL

Range

31.25 - 2000 pg/mL

Assay Platform

Reagents

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Complementary Products

Assay Kits

AB210579

Mouse Pro-Collagen I alpha 1 ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (AB210579)

  • 5
  • 1
Primary Antibodies

AB260043

Anti-Collagen I antibody [EPR22894-89]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-Collagen I antibody [EPR22894-89] (AB260043)

  • 4
  • 3
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
Primary Antibodies

AB227440

Anti-CKMT2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CKMT2 antibody (AB227440)

  • 0
  • 0
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Reactivity data

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Product details

Mouse Pro-Collagen I alpha 1 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse Pro-Collagen I alpha 1.Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our . An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab210579. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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What's included?

{ "values": { "5x96Tests": { "sellingSize": "5 x 96 Tests", "publicAssetCode":"ab216791-5x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Mouse Pro-Collagen I alpha 1 Lyophilized Protein", "number":"AB216791-CMP03", "productcode":"" }, { "size":"1 x 12.5 µg", "name":"Mouse Pro-Collagen I alpha 1 Detector Antibody", "number":"AB216791-CMP02", "productcode":"" }, { "size":"1 x 50 µg", "name":"Mouse Pro-Collagen I alpha 1 Capture Antibody", "number":"AB216791-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collagen type I also called collagen I is a structural protein expressed mainly in connective tissues such as skin tendon bone and ligaments. It serves as an important component in providing mechanical strength and integrity to these tissues. Collagen I is a fibrillar collagen known for its triple-helix structure composed of two alpha-1 chains and one alpha-2 chain and has a molecular mass of approximately 300 kDa. Researchers often employ collagen western blot and collagen ELISA techniques for its detection. Collagen suppliers offer various collagen antibodies used in these assays to study its distribution and function.
Biological function summary

Collagen type I plays a central role in maintaining the extracellular matrix and supporting cellular environments. It interacts with other matrix proteins and cells forming complexes that help in tissue development and repair. Type I collagen is especially important in bone matrix working alongside minerals like hydroxyapatite to provide rigidity and support. Anti-collagen antibodies aid in studying its biological functions and interactions which are critical to understanding tissue dynamics.

Pathways

Collagen type I interacts with multiple signaling cascades involved in tissue remodeling and repair. It is a significant player in the TGF-β pathway which regulates fibrosis and wound healing processes. In these pathways proteins such as fibronectin and integrins work in concert with collagen type I to orchestrate cellular responses to damage. Researchers often examine its role in these pathways to uncover therapeutic possibilities for disease interventions.

Collagen type I has strong connections to conditions like osteogenesis imperfecta and fibrosis. Mutations or irregularities in collagen I production can lead to osteogenesis imperfecta a genetic disorder characterized by brittle bones. In fibrosis excessive collagen deposition disrupts normal tissue architecture contributing to organ dysfunction. In both conditions type I collagen interacts with other proteins like matrix metalloproteinases which modulate its breakdown and remodeling highlighting its importance in disease pathology.
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Product protocols

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Target data

Type I collagen is a member of group I collagen (fibrillar forming collagen).
See full target information Col1a1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular gastroenterology and hepatology 19:101472 PubMed39892785

2025

Cellular Crosstalk Promotes Hepatic Progenitor Cell Proliferation and Stellate Cell Activation in 3D Co-culture.

Applications

Unspecified application

Species

Unspecified reactive species

Maya W Haaker,Jung-Chin Chang,Brian K Chung,Tobias S Pieper,Falko Noé,Tongtong Wang,Niels Geijsen,Martin Houweling,Christian Wolfrum,Arie B Vaandrager,Espen Melum,Bart Spee,J Bernd Helms
View all publications
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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