JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB219524

Mouse Progranulin Matched Antibody Pair Kit

5

(1 Review)

|

(0 Publication)

Mouse Progranulin Matched Antibody Pair Kit is a kit for ELISA development for the measurement of Mouse Progranulin by sandwich ELISA method.

View Alternative Names

Progranulin, PGRN, Acrogranin, Epithelin/granulin precursor, Glycoprotein of 88 Kda, PC cell-derived growth factor, Proepithelin, GP88, Glycoprotein 88, PCDGF, PEPI, Grn

1 Images
ELISA - Mouse Progranulin Matched Antibody Pair Kit (AB219524)
  • ELISA

Supplier Data

ELISA - Mouse Progranulin Matched Antibody Pair Kit (AB219524)

Mouse Progranulin standard curve.

Standard calibration curve. Background subtracted values are graphed.

Key facts

Detection method

Colorimetric

Reacts with

Mouse

Assay type

ELISA set

Sensitivity

= 5.16 pg/mL

Range

15.6 - 1000 pg/mL

Assay Platform

Reagents

style
left-column

Complementary Products

Assay Kits

AB252364

Human Progranulin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Progranulin ELISA Kit (AB252364)

  • 0
  • 0
Assay Kits

AB221440

Mouse Apolipoprotein AI Matched Antibody Pair Kit

Recombinant

ELISA - Mouse Apolipoprotein AI Matched Antibody Pair Kit (AB221440)

  • 0
  • 0
Primary Antibodies

AB187070

Anti-Granulin antibody [EPR18539-59]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Granulin antibody [EPR18539-59] (AB187070)

  • 0
  • 0
Assay Kits

AB218185

Human IL-18 Matched Antibody Pair Kit

Recombinant

Sandwich ELISA - Human IL-18 Matched Antibody Pair Kit (AB218185)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Mouse Progranulin Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse Progranulin. Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification. Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our . An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905). For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab213473. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
style
left-column

What's included?

{ "values": { "5x96Tests": { "sellingSize": "5 x 96 Tests", "publicAssetCode":"ab219524-5x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Mouse Progranulin Lyophilized Protein", "number":"AB219524-CMP03", "productcode":"" }, { "size":"1 x 12.5 µg", "name":"Mouse Progranulin Detector Antibody", "number":"AB219524-CMP02", "productcode":"" }, { "size":"1 x 50 µg", "name":"Mouse Progranulin Capture Antibody", "number":"AB219524-CMP01", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Granulin also known as progranulin or GRN is a protein with a molecular mass of approximately 68 kDa. This protein is encoded by the GRN gene and is involved in various cellular processes. Granulin is expressed widely within the body particularly in epithelial cells immune cells and neurons. Researchers have developed methods like GRN ELISA and progranulin ELISA to quantitatively measure the levels of granulin in biological samples aiding in further understanding of its role in health and disease.
Biological function summary

Granulin functions as a growth factor involved in tissue repair cell proliferation and inflammation. It does not function as part of a larger protein complex but acts individually in exerting its effects on cells. Granulin influences various aspects of cellular architecture and signaling pathways making it important for maintaining cellular homeostasis. Additionally the granulin protein participates in regulating networks that involve cytokines and growth factors impacting both the innate and adaptive immune responses.

Pathways

Granulin plays significant roles in the MAPK and NF-kB signaling pathways. These pathways critically manage cellular reactions to stress inflammation and other external stimuli. Granulin modulates these processes often interacting with proteins like TNF receptors and interleukins to affect the downstream signaling events. Interactions between granulin and other signaling molecules allow it to integrate into broader networks that oversee various biological responses.

Significant links exist between granulin mutations and frontotemporal dementia (FTD) and Alzheimer's disease. Mutations in the GRN gene often lead to reduced granulin levels contributing to neurodegenerative conditions. These mutations affect the protein's normal function promoting pathological mechanisms associated with neuronal deterioration. Granulin interacts with proteins such as TDP-43 in neurodegenerative diseases where alterations might result in protein aggregation and neurotoxicity. Understanding these connections can lead to potential therapeutic interventions targeting granulin-related pathways.
style
left-column

Product protocols

style
left-column

Target data

Secreted protein that acts as a key regulator of lysosomal function and as a growth factor involved in inflammation, wound healing and cell proliferation (PubMed : 12524533, PubMed : 20026663, PubMed : 23041626, PubMed : 27789271, PubMed : 28073925, PubMed : 28453791, PubMed : 28541286, PubMed : 8496151). Regulates protein trafficking to lysosomes and, also the activity of lysosomal enzymes (PubMed : 27789271, PubMed : 28453791, PubMed : 28541286). Facilitates also the acidification of lysosomes, causing degradation of mature CTSD by CTSB (PubMed : 28073925). In addition, functions as a wound-related growth factor that acts directly on dermal fibroblasts and endothelial cells to promote division, migration and the formation of capillary-like tubule structures (PubMed : 12524533). Also promotes epithelial cell proliferation by blocking TNF-mediated neutrophil activation preventing release of oxidants and proteases (PubMed : 8496151). Moreover, modulates inflammation in neurons by preserving neurons survival, axonal outgrowth and neuronal integrity (PubMed : 20026663, PubMed : 23041626).. Granulin-3. Inhibits epithelial cell proliferation and induces epithelial cells to secrete IL-8.. Granulin-7. Stabilizes CTSD through interaction with CTSD leading to maintain its aspartic-type peptidase activity.
See full target information Grn
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com