Munc18-1 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Munc18-1.
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Key facts
- Assay type
- ELISA set
- Reactive species
- Human, Mouse, Rat
- Range
- 0.04 - 10 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Participates in the regulation of synaptic vesicle docking and fusion through interaction with GTP-binding proteins (By similarity). Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. Involved in the release of neurotransmitters from neurons through interacting with SNARE complex component STX1A and mediating the assembly of the SNARE complex at synaptic membranes (PubMed:21445306, PubMed:28821673). May play a role in determining the specificity of intracellular fusion reactions (By similarity).
Alternative names
Syntaxin-binding protein 1, Protein unc-18 homolog 1, Protein unc-18 homolog A, Unc18-1, Unc-18A, Stxbp1
What's included?
Recommended products
Munc18-1 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Munc18-1.
Key facts
- Assay type
- ELISA set
- Reactive species
- Human, Mouse, Rat
- Range
- 0.04 - 10 ng/mL
- Assay Platform
- Reagents
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The pair can be used in variety of assays and platforms including but not limited to:
- Sandwich ELISA
- FRET/TR-FRET/HTRF
- Meso Scale Discovery® (MSD®)
- Bead-based assays
- AlphaLISA®/AlphaScreen®
- DELFIA® immunoassays
- Simoa® and Single Molecule Counting (SMC™) immunoassays
- Multiplex
Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:
- Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
- Concentration of ~1 mg/ml as measured by the protein A280 method.
We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.
Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.
Please note:
The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.
The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.
We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.
Antibody properties:
Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg
Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg
Concentration: ~1 mg/ml
Storage buffer: 100% PBS
Form: Liquid
Isotype: IgG
Recombinant monoclonal antibodies offer several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free production
Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.
AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.
Simoa is a registered trademark of Quanterix, Inc.
SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Munc18-1 also known as Syntaxin-binding protein 1 is an important protein involved in the regulation of neurotransmitter release at synapses. It has a molecular mass of approximately 67 kDa. This protein is highly expressed in the brain where it plays a significant role in synaptic vesicle exocytosis. Munc18-1 interacts with components of the SNARE complex facilitating the docking and fusion of synaptic vesicles with the presynaptic membrane.
- Biological function summary
This protein is important for the proper release of neurotransmitters into the synaptic cleft. Munc18-1 functions as a part of the neuronal exocytosis complex where it regulates the interaction with syntaxin-1 a core SNARE protein. This protein-protein interaction stabilizes an intermediate state necessary for vesicle priming and subsequent fusion ensuring efficient synaptic transmission. By controlling vesicle movement Munc18-1 influences communication between neurons.
- Pathways
Munc18-1 integrates into the neurotransmitter release pathway which is central to synaptic function. It closely associates with the SNARE complex involving proteins such as SNAP-25 and synaptobrevin. Another important pathway is the synaptic vesicle cycle where Munc18-1 plays a role in vesicle docking and priming stages. Through these interactions it ensures that synaptic vesicles are ready and able to release their contents upon stimulation.
- Associated diseases and disorders
Alterations in Munc18-1 expression or function connect to neurological conditions like epilepsy and intellectual disability. In epilepsy dysregulation of neurotransmitter release due to modified Munc18-1 function disrupts synaptic communication leading to seizure activity. In the context of these disorders Munc18-1 interacts functionally with proteins such as VAMP2 contributing to the pathological state when disrupted. These interactions highlight the importance of Munc18-1 in maintaining normal neurological function.
Product promise
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1 product image
Sandwich ELISA - Munc18-1 Antibody Pair - BSA and Azide free (ab318214)
Sandwich ELISA of ab318214 with the capture antibody Anti-Munc18-1 antibody [EPR27956-76] - BSA and Azide free (Capture) ab318215 dilution at 2 ug/mL and detector antibody Anti-Munc18-1 antibody [EPR27956-33] - BSA and Azide free (Detector) ab318216 dilution at 0.5 ug/mL.
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