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Rat TIMP1 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Rat TIMP1.

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Key facts

Assay type

ELISA set

Reactive species

Rat

Range

37.5 - 2400 pg/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Also stimulates steroidogenesis by Leydig and ovarian granuloma cells; procathepsin L is required for maximal activity.

Alternative names

What's included?

10 x 96 Tests
Components
Rat TIMP1 Capture Antibody (unconjugated)
1 x 100 µg
Rat TIMP1 Detector Antibody (unconjugated)
1 x 100 µg

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Rat TIMP1 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Rat TIMP1.

Alternative names

Key facts

Assay type

ELISA set

Reactive species

Rat

Range

37.5 - 2400 pg/mL

Assay Platform

Reagents

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

  • Rat TIMP1 is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify Rat TIMP1 in sandwich ELISAs and many other pair-based applications.
  • The pair can be used in variety of assays and platforms including but not limited to:
  • - Sandwich ELISA
  • - FRET/TR-FRET/HTR
  • - Meso Scale Discovery® (MSD®)
  • - Luminex® and bead-based assays
  • - AlphaLISA®/AlphaScreen®
  • - DELFIA® immunoassays
  • - Simoa® and Single Molecule Counting (SMC™) immunoassays
  • - Multiplex

  • Our antibody pairs are supplied in a a carrier-free format that is conjugation-ready:
  • - Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
  • - Concentration of ~1 mg/ml as measured by the protein A280 method.

  • Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for biotin, HRP, gold, and fluorescent dyes.
  • We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation service

  • Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.

  • Please note:
  • The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.
  • The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.
  • We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.

  • Antibody properties:
  • Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 μg
  • Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 μg
  • Concentration: ~1 mg/ml
  • Storage buffer: 100% PBS
  • Form: Liquid
  • Isotype: IgG

  • Recombinant monoclonal antibodies offer several advantages including:
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free production

  • Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
  • Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.
  • Luminex is a trademark of Luminex Corporation, registered in the US and other countries.
  • AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.
  • Simoa is a registered trademark of Quanterix, Inc.
  • SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

Supplementary info

Biological function summary

TIMP1 plays several essential roles beyond inhibiting metalloproteinases. It supports cell proliferation and influences apoptosis. It is part of a larger TIMP family and does not function as part of a complex per se but interacts closely with matrix metalloproteinases (MMPs). By regulating MMP activity TIMP1 balances processes like tissue growth and inflammatory responses which are important for normal development and repair.

Activity summary

Tissue Inhibitor of Metalloproteinase 1 (TIMP1) is a protein with a molecular mass of approximately 28 kDa. The protein inhibits metalloproteinases by binding to them preventing the breakdown of the extracellular matrix. This action regulates extracellular environment and tissue remodeling. TIMP1 is also known by the name Erythroid-Potentiating Activity (EPA). It is expressed in various tissues including the liver and the brain and can be found in high levels in the blood serum.

Pathways

TIMP1 participates in the regulation of the matrix metalloproteinase pathway impacting tissue homeostasis and repair. It closely associates with proteins such as MMP-9 and MMP-2 within this pathway. TIMP1 also plays a role in cytokine signaling pathways which influence immune responses by interacting with other signaling molecules and receptors that govern these pathways.

Associated diseases and disorders

TIMP1's regulation of MMPs links it to cancer and cardiovascular disease progression. Overexpression of TIMP1 associates with tumor growth and metastasis where it can modulate interactions with other proteins like MMP-9 leading to altered tissue invasion and angiogenesis. Additionally TIMP1 imbalance connects to tissue fibrosis in cardiovascular disorders working in tandem with proteins such as MMP-2 which affects the structural remodeling and function of tissues within the cardiovascular system.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Rat TIMP1 Antibody Pair - BSA and Azide free (ab253779), expandable thumbnail

    Sandwich ELISA - Rat TIMP1 Antibody Pair - BSA and Azide free (ab253779)

    Sandwich ELISA of ab253779 with the capture antibody ab284977 dilution at 5 µg/mL and detector antibody ab285083 dilution at 0.2 µg/mL.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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