SMARCA2 / BRM Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of SMARCA2 / BRM.
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Key facts
- Assay type
- ELISA set
- Reactive species
- Human, Mouse, Rat
- Range
- 1.56 - 50 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Binds DNA non-specifically (PubMed:22952240, PubMed:26601204). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity).
Alternative names
BAF190B, BRM, SNF2A, SNF2L2, SMARCA2, Probable global transcription activator SNF2L2, ATP-dependent helicase SMARCA2, BRG1-associated factor 190B, Protein brahma homolog, SNF2-alpha, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2, hBRM
What's included?
Recommended products
SMARCA2 / BRM Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of SMARCA2 / BRM.
Key facts
- Assay type
- ELISA set
- Reactive species
- Human, Mouse, Rat
- Range
- 1.56 - 50 ng/mL
- Assay Platform
- Reagents
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The pair can be used in variety of assays and platforms including but not limited to:
- Sandwich ELISA
- FRET/TR-FRET/HTRF
- Meso Scale Discovery® (MSD®)
- Bead-based assays
- AlphaLISA®/AlphaScreen®
- DELFIA® immunoassays
- Simoa® and Single Molecule Counting (SMC™) immunoassays
- Multiplex
Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:
- Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
- Concentration of ~1 mg/ml as measured by the protein A280 method.
We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.
Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.
Please note:
The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.
The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.
We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.
Antibody properties:
Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg
Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg
Concentration: ~1 mg/ml
Storage buffer: 100% PBS
Form: Liquid
Isotype: IgG
Recombinant monoclonal antibodies offer several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free production
Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.
AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.
Simoa is a registered trademark of Quanterix, Inc.
SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The SMARCA2 protein also known as BRM (Brahma-related gene 1) is a component of the SWI/SNF chromatin remodeling complex. This protein has a molecular mass of approximately 185 kDa. Scientists observe that SMARCA2/BRM is expressed in various tissues including the brain and liver. It acts as an ATPase which moves histone octamers in chromatin and facilitates access to DNA. SMARCA2/BRM plays an important role in transcriptional activation by modifying chromatin structure.
- Biological function summary
SMARCA2/BRM interacts with other subunits to form the SWI/SNF complex important for regulating gene expression. This complex helps control the transcription of specific genes by repositioning nucleosomes. SMARCA2/BRM modulates cell cycle progression and differentiation as it influences transcription factor access to DNA. Its activity is essential for normal cellular responses maintaining cell homeostasis and development.
- Pathways
SMARCA2/BRM participates significantly in the cell cycle and Wnt signaling pathways. In the cell cycle it cooperates with proteins like p53 to regulate genes involved in cell growth and apoptosis. In the Wnt pathway SMARCA2/BRM interacts with beta-catenin to influence cellular responses to Wnt signals. These pathways are critical for cell proliferation and maintaining cellular integrity and SMARCA2/BRM serves as a pivotal modulator within these systems.
- Associated diseases and disorders
SMARCA2/BRM has a connection to cancer and Coffin-Siris syndrome. Altered expression of SMARCA2/BRM has been observed in several cancers where it frequently interacts with other oncogenic proteins disrupting normal regulatory functions and promoting tumorigenesis. Meanwhile mutations in SMARCA2 can lead to Coffin-Siris syndrome a genetic disorder affecting development and it is connected with other proteins like SOX2 which are also involved in developmental pathways. Understanding SMARCA2's role may uncover insights into therapeutic targets for these conditions.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Sandwich ELISA - SMARCA2 / BRM Antibody Pair - BSA and Azide free (ab315986)
Sandwich ELISA of ab315986 with the capture antibody Anti-SMARCA2 / BRM antibody [EPR26214-254] - BSA and Azide free (Capture) ab315987 dilution at 2 µg/mL and detector antibody Anti-SMARCA2 / BRM antibody [EPR26214-212] - BSA and Azide free (Detector) ab315988 dilution at 0.5 µg/mL.
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