Anti-Fade Fluorescence Mounting Medium - Aqueous, Fluoroshield
5
(1 Review)
|
(31 Publications)
- Proven performance: cited in over 30 publications
- Compatible with our range of fluorescent labeled secondary antibodies
- Available in different sizes to fit your experimental needs
- IHC-FoFr
PubMed
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Fade Fluorescence Mounting Medium - Aqueous, Fluoroshield (AB104135)
Hurley SM, Lutay N, Holmqvist B, Shannon O (2016) The Dynamics of Platelet Activation during the Progression of Streptococcal Sepsis. PLoS ONE 11(9) : e0163531. https : //doi.org/10.1371/journal.pone.0163531
- IHC-FoFr
PubMed
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Fade Fluorescence Mounting Medium - Aqueous, Fluoroshield (AB104135)
Guan-Yu Xiao ,I-Hsuan Liu ,Chun-Chun Cheng,Chia-Chun Chang,Yen-Hua Lee,Winston Teng-Kuei Cheng,Shinn-Chih Wu
Published : September 8, 2014https : //doi.org/10.1371/journal.pone.0106538
Image from Guan-Yu et al., PLoS One, 9(9):e106538; doi: 10.1371/journal.pone.0106538. Reproduced under the Creative Commons license https://creativecommons.org/licenses
- IHC-FoFr
PubMed
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Fade Fluorescence Mounting Medium - Aqueous, Fluoroshield (AB104135)
Dioli C, Patrício P, Sousa N, et al. Chronic stress triggers divergent dendritic alterations in immature neurons of the adult hippocampus, depending on their ultimate terminal fields. Transl Psychiatry. 2019;9(1) : 143. Published 2019 Apr 26. doi : 10.1038/s41398-019-0477-7
Image from Hurley et al., PLoS One, 11(9):e0163531; doi: 10.1371/journal.pone.0163531. Reproduced under the Creative Commons license https://creativecommons.org/licenses
Product details
Fluoroshield is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This unique formula prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), tetramethyly rhodamine, Redox, phycoerythrin (R-PE), phyocyanin (PC), and allophycocyanin (APC). The fluorescence is retained during prolonged storage at 4°C in the dark. This medium does not contain phenylenediamine, which destroys immunofluorescence of Cy dyes, R-PE, PC and APC.
Mounting media products
For fluorescent cell and tissue staining, Abcam recommends this product (Fluorescence Mounting Medium ab104135), Mounting Medium with DAPI ab104139, and Mounting Medium with PI ab104129. For thick sections or tissues containing lots of fat, we recommend Glycerol Mounting Medium with DAPI ab188804.
For chromogenic immunohistochemistry, such as with DAB, AEC, or Fast Red, we recommend aqueous Mounting Medium ab64230, or organic Limonene Mounting Medium ab104141.
Procedure
- Bring the vial to room temperature.
- Rinse slide to be mounted with distilled or deionized, touch the edges of slide on a paper towel to remove excess water. Place slides on a flat surface.
- Turn the vial upside down and open the dropper to remove any air bubbles.
- Apply 3-4 drops of mounting medium directly on top of the specimen and spread out evenly by tilting slide back and forth or spread evenly with a 0.2 ml plastic pipette tip making sure the tissue is not touched. Excess medium can be removed by touching the edges of slide against paper towel.
- Let stand at room temperature for about 5 minutes.
- Apply cover slip carefully avoiding air bubbles.
- The specimen is ready for visualization under a microscope.
- One can seal the edges of cover slip with nail polish, any organic medium or our Organo mounting medium (ab104141). If a coverslip is not sealed air bubbles will appear in few days.
- For storage it is recommended that the slide be stored in the dark at 2-8°C.
Removal of Coverslip
Coverslip can be removed before sealing the edges. Soak slide in warm (37°C) distilled or deiononized water for several minutes. Carefully and slowly move the coverslip. Soak in water for an additional few minutes to remove coverslip. Rinse slide several times with warm water to remove all mounting medium. The slide can be remounted again.
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Target data
Publications (31)
Recent publications for all applications. Explore the full list and refine your search
Brain sciences 11: PubMed33806692
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Frontiers in molecular neuroscience 13:574041 PubMed33551740
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Frontiers in bioengineering and biotechnology 8:614357 PubMed33520965
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Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi 56:58-68 PubMed33132281
2020
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Allergy, asthma & immunology research 12:980-993 PubMed32935490
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PloS one 15:e0238208 PubMed32881928
2020
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Cell reports 32:107973 PubMed32726636
2020
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Nature communications 11:3154 PubMed32572025
2020
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Bioengineering (Basel, Switzerland) 7: PubMed32545910
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Neurobiology of aging 92:98-113 PubMed32417750
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