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AB133411

Low Fluorescence Western Membrane (PVDF)

5

(1 Review)

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(7 Publications)

Low Fluorescence Western Membrane - 0.45 µm (PVDF) (ab133411) are high quality, durable polyvinylidene difluoride (PVDF) pre-cut membranes (10 sheets) and suitable for immunoblotting applications.

- Suitable for western transfer with fluorescent detection
- Detection methos: Fluorescence, total protein stain.
- High signal-to noise rations– with low background levels
- Compatible with gels 7 x 9 cm and smaller

Key facts

Applications

WB

applications

Form

Solid

form

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Pre-cut membranes (10 sheets, 7 x 9 cm), compatible with gels 10 x 10 cm and smaller. Note: PVDF membranes must be activated prior to use. Membrane activation protocol: 1. Pre-wet the membrane in methanol for a few moments until the membrane is completely wet. 2. Transfer the wet membrane from methanol to purified water and incubate on a rocker or shaker for at least 5 minutes. Make sure that the membrane is fully immersed in water and does not float on the water's surface. 3. Transfer the membrane from water to transfer buffer, and incubate for 5 minutes.
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
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Product protocols

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Target data

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 22: PubMed33925309

2021

Simulated Microgravity Inhibits the Proliferation of Chang Liver Cells by Attenuation of the Major Cell Cycle Regulators and Cytoskeletal Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Chi Nguyen Quynh Ho,Minh Thi Tran,Chung Chinh Doan,Son Nghia Hoang,Diem Hong Tran,Long Thanh Le

Journal of cellular and molecular medicine 25:3449-3459 PubMed33660415

2021

Neuroprotective effect of ketamine against TNF-α-induced necroptosis in hippocampal neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Wang,Bin Deng,Panpan Yan,Huanghui Wu,Chunhui Li,Hongrui Zhu,Jiwei Du,Lichao Hou

Bioscience reports 41: PubMed33350435

2020

Optimization of the isolation procedure and culturing conditions for hepatic stellate cells obtained from mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Thanh Minh Dang,Trinh Van Le,Huy Quang Do,Van Thuan Nguyen,Ai Xuan Le Holterman,Loan Tung Thi Dang,Nhan Chinh Lu Phan,Phuc Van Pham,Son Nghia Hoang,Long Thanh Le,Gabriele Grassi,Nhung Hai Truong

Medical science monitor : international medical jo 25:9963-9972 PubMed31927560

2019

Naringin Protects Against Interleukin 1β (IL-1β)-Induced Human Nucleus Pulposus Cells Degeneration via Downregulation Nuclear Factor kappa B (NF-κB) Pathway and p53 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Gao,Feng Chang,Ting Zhang,Xinhu Huang,Chen Yu,Zhaolin Hu,Mingming Ji,Yufen Duan

Cancer chemotherapy and pharmacology 83:1017-1024 PubMed30888463

2019

D,L-Methadone does not improve radio- and chemotherapy in glioblastoma in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Henry Oppermann,Martina Matusova,Annegret Glasow,Johannes Dietterle,Rainer Baran-Schmidt,Karsten Neumann,Jürgen Meixensberger,Frank Gaunitz

Mutation research. Genetic toxicology and environmental mutagenesis 840:11-19 PubMed30857728

2019

Lactobacillus plantarum MON03 counteracts zearalenone génotoxicty in mice: Chromosome aberrations, micronuclei, DNA fragmentation and apoptotique gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Hela Belgacem,Jalila Ben Salah-Abbès,Khawla Ezzdini,Mosaad A Abdel-Wahhab,Abdellah Zinedine,Samir Abbès

Toxicon : official journal of the International Society on Toxinology 146:13-23 PubMed29574215

2018

Lactobacillus plantarum alleviate aflatoxins (B and M) induced disturbances in the intestinal genes expression and DNA fragmentation in mice.

Applications

WB

Species

Unspecified reactive species

Rania Jebali,Jalila Ben Salah-Abbès,Samir Abbès,Aziza M Hassan,Sekena H Abdel-Aziem,Aziza A El-Nekeety,Ridha Oueslati,Mosaad A Abdel-Wahhab
View all publications
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