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Insulin antibody. Suitable for AP, IHC-P, IHC-Fr and reacts with samples. Cited in 4 publications.

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Images

Thin Layer Chromatography - Anti-Insulin Affibody® Molecule (AB31906), expandable thumbnail
  • SDS-PAGE - Anti-Insulin Affibody® Molecule (AB31906), expandable thumbnail

Publications

Key facts

Storage buffer

pH: 7.4
Constituents: 0.079% Ammonium bicarbonate

Form

Liquid

Consider this alternative

Reactivity data

Application

AP

Reactivity

Reacts

Dilution info

-

Notes

-

Application

IHC-P

Reactivity

Reacts

Dilution info

-

Notes

-

Application

IHC-Fr

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Alternative names

Recommended products

Insulin antibody. Suitable for AP, IHC-P, IHC-Fr and reacts with samples. Cited in 4 publications.

Key facts

Form

Liquid

Concentration
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Purification notes

ab31906 is >98% pure, as determined by SDS-PAGE (Coomassie blue staining) and RP-HPLC analysis.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Notes

ab31906 is a recombinant protein produced in E. coli.

What are Affibody Molecules?
Affibody® affinity ligands are small, simple proteins composed of a three-helix bundle based on the scaffold of one of the IgG-binding domains of Protein A. Protein A is a surface protein from the bacterium Staphylococcus aureus. This scaffold has excellent features as an affinity ligand and can be designed to bind with high affinity to any given target protein. The domain consists of 58 amino acids, 13 of which are randomized to generate Affibody® libraries with a large number of ligand variants. Thus, the libraries consist of a multitude of protein ligands with an identical backbone and variable surface- binding properties. The current Affibody® libraries contains billions of variants. In function, Affibody® molecules mimic antibodies, nature's own binders to an infinite number of antigens. Compared to antibodies, the most striking dissimilarity of Affibody® molecules is the small size. Affibody® molecules have a molecular weight of 14 kDa, compared to the molecular weight of antibodies, which is 150 kDa. In spite of its small size, the binding site of Affibody® molecules is similar to that of an antibody. The advantages of Affibody® molecules over antibodies are · their small size · the simple structure of the molecules · its robust physical properties · its ability to fold correctly intracellularly · the fast and cost-efficient production in bacteria · the possibility to produce Affibody® molecules through chemical synthesis · the possibility to couple Affibody® molecules in multimeric constructs.

This Anti-Insulin Affibody® Molecule is modified with a unique C-terminal cysteine for directed single-point chemical modification, facilitating labelling with fluorescent dyes, biotin or coupling to matrices. However, tail-to-tail dimers are spontaneously generated via a disulphide bridge between the C-terminal cysteines. Prior to coupling via the C-terminal the Affibody® Molecule needs to be reduced to expose the reactive cysteine residue. Recommended reducing condition is 20mM DTT at a pH above 7.5 and incubation at room temperature for 2 hours. Remove excess DTT by passage through a desalting column, not by dialysis. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

THIS AFFIBODY® MOLECULE REQUIRES CONJUGATION TO A SUITABLE LABEL BEFORE USE.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Insulin a peptide hormone plays an essential mechanical role in carbohydrate and fat metabolism. Insulin alternatively known as bovine insulin in some research contexts has a molecular mass of around 5800 Da. Beta cells in the pancreas express insulin where it gets secreted directly into the bloodstream. The hormone regulates glucose levels by facilitating the uptake of glucose into cells such as muscle and adipose tissues.

Biological function summary

Insulin influences several key metabolic processes by binding to its receptor a component of the insulin receptor substrate family. This triggers a cascade of events within cells promoting the uptake of glucose and converting it into energy. Insulin operates as a singular entity but plays a critical role in forming a complex with its receptor. This interaction triggers downstream effects that alter the activity of enzymes and transcription factors involved in glucose and lipid metabolism.

Pathways

Insulin is critical for the regulation of the PI3K-AKT signaling pathway and the MAPK pathway. These pathways modulate processes such as cell growth proliferation and survival. Insulin interacts closely with other proteins in these pathways such as the insulin receptor substrate proteins and influences downstream proteins like AKT kinase which has a significant role in mediating the metabolic effects initiated by insulin binding.

Associated diseases and disorders

Insulin is most notably linked to diabetes mellitus characterized by insufficient insulin production or action. This deficiency disrupts normal glucose homeostasis leading to hyperglycemia. Other associated proteins include glucagon which counteracts insulin's role in lowering blood glucose levels and IRS-1 an important element through which insulin signaling is transduced. Researchers utilize mouse insulin ELISA and insulin ELISA kits to better understand these relationships and develop treatments for insulin-related disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Thin Layer Chromatography - Anti-Insulin Affibody® Molecule (ab31906), expandable thumbnail

    Thin Layer Chromatography - Anti-Insulin Affibody® Molecule (ab31906)

    To demonstrate the binding capacity and specificity of the Anti-Insulin Affibody® molecule, 1.5 ml of five times
    diluted human serum spiked with insulin was injected on a column with 0.4 ml SulfoLink® Coupling gel with
    immobilized Anti-Insulin Affibody® molecule.

  • SDS-PAGE - Anti-Insulin Affibody® Molecule (ab31906), expandable thumbnail

    SDS-PAGE - Anti-Insulin Affibody® Molecule (ab31906)

    Eluted
    and flow-through fractions were analyzed by an SDS-PAGE analysis:



    Lane 1: human serum spiked with insulin

    Lane 2: flowthrough fraction

    Lane 3: eluted fraction

    Lane 4: human insulin standard

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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