JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB229278

Salmon Sperm ssDNA

Be the first to review this product! Submit a review

|

(1 Publication)

Salmon Sperm ssDNA (ab229278) is a ready-to-use solution of salmon sperm sheared single-stranded DNA. Suitable to use in the preparation of pre-hybridization and hybridization solutions and as a DNA carrier in yeast transformation protocols and other related methods

- Compatible as block for nonspecific pre-hybridization and hybridization solutions
- DNA sheared to average size of 100 to 200 base pairs

Key facts

Applications

Flow Cyt

applications

Form

Liquid

form

Storage buffer

pH: 7.6 Constituents: 0.16% Tris HCl, 0.03% EDTA

storage-buffer

style
left-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Reagents, Controls & Accessories

AB210904

Blocking Buffer 10X

  • 0
  • 0
Primary Antibodies

AB420

Anti-Digoxigenin antibody [21H8]

  • 0
  • 0
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Flow Cyt": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

This product is a convenient, ready-to-use solution of salmon sperm DNA especially prepared for use in the preparation of pre-hybridization and hybridization solutions and as a DNA carrier in yeast transformation protocols and other related methods. This solution contains sheared single-stranded DNA molecules that can be used to block the non-specific attachment of probe DNA to the surface of a membrane (Southern) or to increase yeast transformation efficiency.

Background: Prepared by a modification of the method of Emanuel and Chaikoff, JBC 203, 164 (1953) from salmon testes DNA by mechanical shearing and heat denaturation to an average size of 100 to 200 base pairs. To reverse any renaturation occurring during storage, this material should be briefly boiled and rapidly chilled prior to use. >75% native nucleic acid.

Purity/Specifity: A260/A280: 1.85 % Hyperchromocity: 30.5.

style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

style
left-column

Target data

style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature methods 17:833-843 PubMed32632238

2020

ZipSeq: barcoding for real-time mapping of single cell transcriptomes.

Applications

Unspecified application

Species

Unspecified reactive species

Kenneth H Hu,John P Eichorst,Chris S McGinnis,David M Patterson,Eric D Chow,Kelly Kersten,Stephen C Jameson,Zev J Gartner,Arjun A Rao,Matthew F Krummel
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com