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This product is a convenient, ready-to-use solution of salmon sperm DNA especially prepared for use in the preparation of pre-hybridization and hybridization solutions and as a DNA carrier in yeast transformation protocols and other related methods.

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Publications

Key facts

Applications
Flow Cyt
Form
Liquid

Reactivity data

Application
Flow Cyt
Reactivity
Expected
Dilution info
-
Notes

-

Recommended products

This product is a convenient, ready-to-use solution of salmon sperm DNA especially prepared for use in the preparation of pre-hybridization and hybridization solutions and as a DNA carrier in yeast transformation protocols and other related methods.

Key facts

Applications
Flow Cyt
Form
Liquid
Storage buffer

pH: 7.6
Constituents: 0.16% Tris HCl, 0.03% EDTA

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a convenient, ready-to-use solution of salmon sperm DNA especially prepared for use in the preparation of pre-hybridization and hybridization solutions and as a DNA carrier in yeast transformation protocols and other related methods. This solution contains sheared single-stranded DNA molecules that can be used to block the non-specific attachment of probe DNA to the surface of a membrane (Southern) or to increase yeast transformation efficiency.

Background: Prepared by a modification of the method of Emanuel and Chaikoff, JBC 203, 164 (1953) from salmon testes DNA by mechanical shearing and heat denaturation to an average size of 100 to 200 base pairs. To reverse any renaturation occurring during storage, this material should be briefly boiled and rapidly chilled prior to use. >75% native nucleic acid.

Purity/Specifity: A260/A280: 1.85 % Hyperchromocity: 30.5.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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