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Apoptosis and DNA Damage (H2A.X(S139) + cleaved PARP1 + Anti-GAPDH) Western Blot Cocktail (ab131385) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
Alternative names=Histone H2AX, H2a/x, Histone H2A.X, H2AFX, H2AX, H2AS139p, H2AXS139p, H2A.XS139p
- Applications
WB
- Target
H2AX phospho S139
- Reactive species
Human
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Reacts | Dilution info - | Notes Entire WB procedure to be done in 4% milk/PBS. Note that pH2A.X is ~16 kDa, hence do not run the SDS-PAGE gel too long. Use anti-Mouse-HRP secondary antibody. |
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Apoptosis and DNA Damage (H2A.X(S139) + cleaved PARP1 + Anti-GAPDH) Western Blot Cocktail (ab131385) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
Alternative names=Histone H2AX, H2a/x, Histone H2A.X, H2AFX, H2AX, H2AS139p, H2AXS139p, H2A.XS139p
Key facts
- Applications
WB
- Target
H2AX phospho S139
- Reactive species
Human
- Purity
IgG fraction
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
Notes
The Apoptosis and DNA Damage (H2A.X(S139) + cleaved PARP1 + Anti-GAPDH) Western Blot Cocktail (ab131385) is designed to study the induction of DNA damage and/or apoptosis in response to various stimuli. The two main components of this cocktail are monoclonal antibodies specific to cleaved-PARP1 and H2A.X phospho Ser139. H2A.X is a histone H2A family member that is phosphorylated and recruited to sites of double-strand DNA breaks. Poly [ADP-ribose] polymerase 1 (PARP1) is a DNA repair enzyme that is cleaved by activated caspases. Combined, these antibodies provide biomarkers of dsDNA breaks (H2A.X phospho Ser139) and apoptosis (cleaved-PARP1). An anti-GAPDH antibody is included as a loading control. These three readouts are easily resolved by western blot given their different molecular weights.
Individual antibodies within the ab131385 cocktail:
Mouse phospho-H2A.X (pSer139) [9F3] monoclonal, IgG
Working concentration: 1 μg/ml
Mouse cleaved-PARP1 [4B5BD2] monoclonal, IgG1
Working concentration: 1 μg/ml
Mouse GAPDH [3E8AD9] monoclonal, IgG2b:
Working concentration: 0.1 μg/ml
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