Apoptosis Western Blot Cocktail (pro/cleaved Caspase-3, cleaved PARP1, Muscle Actin) (ab136812). For detection of the apoptosis biomarkers Caspase 3 and cleaved PARP, along with the loading control muscle actin.
- Cited in over 30 publications
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- Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology 22:2037-20532023The synthesis of novel water-soluble zinc (II) phthalocyanine based photosensitizers and exploring of photodynamic therapy activities on the PC3 cancer cell line.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNagihan Kocaağa,Ayşegül Türkkol,Mehmet Dinçer Bilgin,Ali ErdoğmuşPubMed 37166570
- Experimental and therapeutic medicine 25:1272023MiR‑221 and miR‑222 regulate cell cycle progression and affect chemosensitivity in breast cancer by targeting ANXA3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJu-Yeon Kim,Eun Jung Jung,Jae-Myung Kim,Youngsim Son,Han Shine Lee,Seung-Jin Kwag,Ji-Ho Park,Jin-Kyu Cho,Han-Gil Kim,Taejin Park,Sang-Ho Jeong,Chi-Young Jeong,Young-Tae JuPubMed 36845963
- Oncology reports 49:2023AZD6738 promotes the tumor suppressive effects of trifluridine in colorectal cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShinnosuke Harata,Takuya Suzuki,Hiroki Takahashi,Takahisa Hirokawa,Akira Kato,Kaori Watanabe,Takeshi Yanagita,Hajime Ushigome,Kazuyoshi Shiga,Ryo Ogawa,Akira Mitsui,Masahiro Kimura,Yoichi Matsuo,Shuji TakiguchiPubMed 36734271
- Physiological reports 11:e155492023β3 adrenergic agonism: A novel pathway which improves right ventricular-pulmonary arterial hemodynamics in pulmonary arterial hypertension.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKeyvan Karimi Galougahi,Yunjia Zhang,Vivian Kienzle,Chia-Chi Liu,Lake-Ee Quek,Sanjay Patel,Edmund Lau,Rachael L Cordina,Gemma A Figtree,David S CelermajerPubMed 36597221
- Oncology reports 47:2022ATR inhibitor AZD6738 increases the sensitivity of colorectal cancer cells to 5‑fluorouracil by inhibiting repair of DNA damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTakuya Suzuki,Takahisa Hirokawa,Anri Maeda,Shinnosuke Harata,Kaori Watanabe,Takeshi Yanagita,Hajime Ushigome,Nozomi Nakai,Yuzo Maeda,Kazuyoshi Shiga,Ryo Ogawa,Akira Mitsui,Masahiro Kimura,Yoichi Matsuo,Hiroki Takahashi,Shuji TakiguchiPubMed 35191521
- International journal of oncology 60:2021Synergism of a novel MCL‑1 downregulator, acriflavine, with navitoclax (ABT‑263) in triple‑negative breast cancer, lung adenocarcinoma and glioblastoma multiforme.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnbok Lee,Hyeon-Ok Jin,Md Masudul Haque,Hee Yeon Kim,Hana Jung,Jin Hee Park,Ilwhan Kim,Joo Yeon Song,Hye Kyoung Yoon,Hyoung Kyu Kim,Jin Han,In-Chul Park,Kwang Seok Kim,Sae Gwang ParkPubMed 34913076
- Free radical biology & medicine 179:242-2512021Advanced photodynamic therapy with an engineered M13 phage targeting EGFR: Mitochondrial localization and autophagy induction in ovarian cancer cell lines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBarbara Bortot,Maura Apollonio,Gabriele Baj,Laura Andolfi,Luisa Zupin,Sergio Crovella,Matteo di Giosia,Andrea Cantelli,Roberto Saporetti,Luca Ulfo,Annapaola Petrosino,Giovanni Di Lorenzo,Federico Romano,Giuseppe Ricci,Maurizio Mongiat,Alberto Danielli,Matteo Calvaresi,Stefania BiffiPubMed 34808331
- International journal of molecular sciences 22:2021Mesenchymal Stem Cell-Conditioned Media Regulate Steroidogenesis and Inhibit Androgen Secretion in a PCOS Cell Model via BMP-2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRishi Man Chugh,Hang-Soo Park,Sahar Esfandyari,Amro Elsharoud,Mara Ulin,Ayman Al-HendyPubMed 34502090
- International journal of molecular sciences 22:2021Copper(II) Complexes Containing Natural Flavonoid Pomiferin Show Considerable In Vitro Cytotoxicity and Anti-inflammatory Effects.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJán Vančo,Zdeněk Trávníček,Jan Hošek,Tomáš Malina,Zdeněk DvořákPubMed 34299247
- Current protocols 1:e1802021CAGE-prox: A Unified Approach for Time-Resolved Protein Activation in Living Systems.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Wang,Yuan Liu,Yanjun Liu,Chu Wang,Peng R ChenPubMed 34165886
Key facts
- Applications
- WB
- Target
- CASP3
(See target data below) - Reactive species
- Human
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Reacts | Dilution info - | Notes 1/250 dilution for primary antibodies. 1/100 dilution for secondary antibodies. Suggested dilution buffer: 5% milk/PBS+0.05% Tween 20. |
Target data
Function
Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis (PubMed:18723680, PubMed:20566630, PubMed:23650375, PubMed:35338844, PubMed:35446120, PubMed:7596430). Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins (PubMed:18723680, PubMed:20566630, PubMed:23650375, PubMed:7596430). At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase PARP1 at a '216-Asp-|-Gly-217' bond (PubMed:10497198, PubMed:16374543, PubMed:7596430, PubMed:7774019). Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain (By similarity). Cleaves and activates caspase-6, -7 and -9 (CASP6, CASP7 and CASP9, respectively) (PubMed:7596430). Cleaves and inactivates interleukin-18 (IL18) (PubMed:37993714, PubMed:9334240). Involved in the cleavage of huntingtin (PubMed:8696339). Triggers cell adhesion in sympathetic neurons through RET cleavage (PubMed:21357690). Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress (PubMed:23152800). Acts as an inhibitor of type I interferon production during virus-induced apoptosis by mediating cleavage of antiviral proteins CGAS, IRF3 and MAVS, thereby preventing cytokine overproduction (PubMed:30878284). Also involved in pyroptosis by mediating cleavage and activation of gasdermin-E (GSDME) (PubMed:35338844, PubMed:35446120). Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface (PubMed:23845944, PubMed:33725486). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC (PubMed:36758104, PubMed:36758106).
Additional Targets
PARP1, ACTC1
Alternative names
CPP32, CASP3, Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CPP-32, SCA-1
Recommended products
Apoptosis Western Blot Cocktail (pro/cleaved Caspase-3, cleaved PARP1, Muscle Actin) (ab136812). For detection of the apoptosis biomarkers Caspase 3 and cleaved PARP, along with the loading control muscle actin.
- Cited in over 30 publications
Key facts
- Applications
- WB
- Target
- CASP3
- Reactive species
- Human
Storage
- Shipped at conditions
- Blue Ice
- Appropriate long-term storage conditions
- +4°C
Notes
ab136812 contains a cocktail of primary antibodies for the detection of apoptosis biomarkers caspase 3 and PARP, along with loading control muscle actin (42 kDa). The caspase 3 antibody (rabbit monoclonal) detects both the 32 kDa pro-caspase 3 as well as the p17 subunit of active caspase 3 generated by cleavage of the pro-caspase 3 at Asp175. The PARP antibody (mouse monoclonal) detects only the apoptosis-specific 89 kDa PARP fragment (cleaved-PARP) generated from the full length PARP by active caspases. Since the primary antibodies used are both mouse and rabbit, a secondary antibodies cocktail of GAM-HRP and GAR-HRP is provided.
The Apoptosis western blot cocktail (ab136812) is designed to study the induction of apoptosis in response to various stimuli. The two main components of this cocktail are monoclonal antibodies specific to caspase 3 and PARP. Caspase 3 is one of the executioner caspases activated by proteolytic cleavage during apoptosis. The rabbit caspase 3 antibody of this cocktail detects both the 32 kDa pro-caspase 3 as well as the p17 subunit of the active caspase 3 generated by cleavage of the pro-caspase 3 at Asp175. Thus the induction of apoptosis can be followed by a decrease of the pro-caspase 3 or by an increase of the p17 caspase 3. Monitoring the changes in the pro-caspase 3 is particularly advantageous, since the proportion of caspase activation can be determined from the reduction of the pro-form from analysis of control and stimulated samples. Poly [ADP-ribose] polymerase 1 (PARP) is a DNA repair enzyme that is cleaved during apoptosis by activated caspases. The mouse PARP antibody of this cocktail detects only the apoptosis-specific 89 kDa PARP fragment (cleaved-PARP). This antibody does not react with the full-length PARP. Combined, these two antibodies provide biomarkers of apoptosis. The rabbit muscle actin antibody is provided as a loading control for sample to sample normalization. Since the primary antibodies are both mouse and rabbit, the cocktail of HRP-conjugated goat anti-rabbit and anti-mouse secondary antibodies is provided for convenience. The targets are easily resolved by Western blot given their different molecular weights.
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2 product images
Western blot - Apoptosis Western Blot Cocktail (pro/p17-caspase-3, cleaved PARP1, muscle actin) (ab136812)
Lanes 1, 3, 5, 7: (HeLa Apoptosis Lysate Set: Staurosporine-Treated and Vehicle-Treated Control ab136806) HeLa, vehicle treated
Lanes 2, 4, 6, 8: (HeLa Apoptosis Lysate Set: Staurosporine-Treated and Vehicle-Treated Control ab136806) HeLa, 1 µM staurosporine (Staurosporine, Protein kinase inhibitor ab120056), 4 hours
All lysates at 20 µg per lane.
Primary antibodies
Lanes 1, 2: Cleaved PARP
Lanes 3, 4: Actin
Lanes 5, 6: Caspase 3
Lanes 7, 8: ab136812 250X Primary Antibodies Cocktail, 1/250 dilution
Secondary antibodies
All lanes: ab136812 100X HRP-Conjugated Secondary Antibodies Cocktail, 1/100 dilution.
Western blot - Apoptosis Western Blot Cocktail (pro/p17-caspase-3, cleaved PARP1, muscle actin) (ab136812)
Lane 1: Jurkat cells, untreated
Lane 2: Jurkat cells treated with anti-FAS for 2 hours
Lane 3: Jurkat cells treated with anti-FAS for 4 hours
Lane 4: Jurkat cells treated with anti-FAS for 6 hours
All lysates at 20 μg/lane
Primary antibodies
All lanes: 250X Primary Antibodies Cocktail, 1/250 dilution.
Secondary antibodies
All lanes: 100X HRP-Conjugated Secondary Antibodies Cocktail (ab136812), 1/100 dilution.
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