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Complex II WB Antibody Cocktail (ab110410) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

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Applications

WB

Reactive species

Mouse, Rat, Cow, Human

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Reactivity data

Application
WB
Reactivity
Reacts
Dilution info
-
Notes

The antibody cocktail (1.5 mg/mL) should be diluted 250x to a final working concentration of 6 µg/mL for Western blotting.

Images

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Complex II WB Antibody Cocktail (ab110410) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

Key facts

Applications

WB

Reactive species

Mouse, Rat, Cow, Human

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

Contains a total of 305 μg of antibodies and is a pre-mixed solution of 3 monoclonal antibodies against Complex II subunits 70 kDa Fp, 30 kDa Ip along with an antibody against Complex V alpha as a control. This cocktail can be used to verify isolated Complex II purity or to conveniently determine the levels of 2 of the 4 Complex II subunits in cell lines relative to the control protein (Complex V alpha).

Cocktail Antibodies:

Mouse C-V-alpha [15H4C4] monoclonal:

Amount: 50 μg

Working concentration: 1 μg/ml

Mouse C-II-30 (FeS) [21A11AE7] monoclonal:

Amount: 250 μg

Working concentration: 5 μg/ml

Mouse C-II-70 (FL) [2E3GC12FB2AE2] monoclonal:

Amount: 5 μg

Working concentration: 0.1 μg/ml

**Related products**Review the , or the full to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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