AB110410

Complex II WB Antibody Cocktail

Name: Complex II WB Antibody Cocktail (ab110410) | Abcam
Brand: Abcam Limited
SKU: AB110410
Price: 630 USD
Availability: InStock

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(39 Publications)

Complex II WB Antibody Cocktail (ab110410) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

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Supplier Data

Western blot - Complex II WB Antibody Cocktail (AB110410)

The Western blot cocktail ab110410 contains two Complex II subunits SDHA (70 kDa) and SDHB (30 kDa) and a general mitochondrial marker ATP synthase alpha subunit (F1α, 55 kDa). All three are present in the mitochondrial sample (1). The Complex II sample purified by Complex II immunoprecipitation beads (ab109799) shows only Complex II subunits (2).

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Key facts

Applications

applications

Reacts with

Mouse, Rat, Cow, Human

Form

Liquid

form

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: HEPES

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>The antibody cocktail (1.5 mg/mL) should be diluted 250x to a final working concentration of 6 µg/mL for Western blotting.</p>" } } }

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Product details

Contains a total of 305 μg of antibodies and is a pre-mixed solution of 3 monoclonal antibodies against Complex II subunits 70 kDa Fp, 30 kDa Ip along with an antibody against Complex V alpha as a control. This cocktail can be used to verify isolated Complex II purity or to conveniently determine the levels of 2 of the 4 Complex II subunits in cell lines relative to the control protein (Complex V alpha).

Cocktail Antibodies:

Mouse C-V-alpha [15H4C4] monoclonal:

Amount: 50 μg

Working concentration: 1 μg/ml

Mouse C-II-30 (FeS) [21A11AE7] monoclonal:

Amount: 250 μg

Working concentration: 5 μg/ml

Mouse C-II-70 (FL) [2E3GC12FB2AE2] monoclonal:

Amount: 5 μg

Working concentration: 0.1 μg/ml

**Related products**Review the , or the full to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Complex II also known as succinate dehydrogenase (SDH) or succinate-ubiquinone oxidoreductase plays an important mechanical role in the mitochondrial electron transport chain. It catalyzes the oxidation of succinate to fumarate while reducing ubiquinone to ubiquinol. The enzyme complex is approximately 140 kDa in mass and resides in the inner mitochondrial membrane. Complex II is expressed in most tissues particularly in high-energy demand tissues such as the heart and skeletal muscles.

Biological function summary

Complex II functions as part of the larger electron transport chain complex and plays a role in the Krebs cycle. It links two critical metabolic pathways converting succinate to fumarate while transferring electrons to the electron transport chain. This makes it integral for proper cellular respiration and energy production. The complex consists of multiple subunits and utilizes co-factors like FAD and iron-sulfur clusters for enzymatic activity. It is also a part of the supercomplexes that optimize the efficiency of oxidative phosphorylation.

Pathways

Complex II plays a significant role in both the citric acid cycle and oxidative phosphorylation. It acts as a connecting bridge between these two pathways facilitating the flow of electrons. Complex II works alongside other proteins such as complex I and complex III to maintain the electron transport chain's function and energy production. Succinate dehydrogenase transfers electrons within the chain directly affecting the generation of ATP by complex V (ATP synthase).

Dysfunction of complex II is associated with mitochondrial diseases and cancers. Mutations or deficiencies in its subunits can lead to conditions like Leigh syndrome and hereditary paraganglioma. These conditions frequently involve other mitochondrial proteins and complexes such as complex I which can exacerbate the electron transport chain dysfunction. In cancers alterations in succinate dehydrogenase activity can result in oncogenic metabolisms by falsely stabilizing hypoxia-inducible factors linking it further with the genetic and metabolic regulation.

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Product protocols

Visit the General protocols
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Target data

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Publications (39)

Recent publications for all applications. Explore the full list and refine your search

Antioxidants (Basel, Switzerland) 14: PubMed40298692

2025

Dysfunctional Mitochondria Characterize Amyotrophic Lateral Sclerosis Patients' Cells Carrying the p.G376D Pathogenetic Substitution.

Applications

Unspecified application

Species

Unspecified reactive species

Giuseppe Petito,Victoria Stefania Del Fiore,Arianna Cuomo,Federica Cioffi,Gilda Cobellis,Antonia Lanni,Flora Guerra,Cecilia Bucci,Rosalba Senese,Roberta Romano

Cell biology and toxicology 41:7 PubMed39707117

2024

METTL3, m6A modification, and EGR1: interplay affecting myocardial I/R injury outcomes.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Huang,Xun Zhang,Shi-Xiong Wu,Qing Chang,Zhi-Kun Zheng,Jing Xu

Brain sciences 12: PubMed35884650

2022

Energy Metabolic Disorder of Astrocytes May Be an Inducer of Migraine Attack.

Applications

Unspecified application

Species

Unspecified reactive species

Junhua Li,Xiaotong Ye,Yang Zhou,Shiqiao Peng,Peibing Zheng,Xiaoxiao Zhang,Jiajun Yang,Yanhong Xu

Current research in pharmacology and drug discovery 3:100087 PubMed35146419

2022

GSK2606414 attenuates PERK/p-eIF2α/ATF4/CHOP axis and augments mitochondrial function to mitigate high glucose induced neurotoxicity in N2A cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chayanika Gundu,Vijay Kumar Arruri,Bhoomika Sherkhane,Dharmendra Kumar Khatri,Shashi Bala Singh

Journal of inflammation (London, England) 18:31 PubMed34930287

2021

Ginsenoside Rg3 alleviates septic liver injury by regulating the lncRNA TUG1/miR-200c-3p/SIRT1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Pan Wu,Xiao Yu,Yue Peng,Qian-Lu Wang,Long-Tian Deng,Wei Xing

Frontiers in immunology 12:700431 PubMed34858390

2021

The Circadian Clock Protein BMAL1 Acts as a Metabolic Sensor In Macrophages to Control the Production of Pro IL-1β.

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Unspecified application

Species

Unspecified reactive species

George A Timmons,Richard G Carroll,James R O'Siorain,Mariana P Cervantes-Silva,Lauren E Fagan,Shannon L Cox,Eva Palsson-McDermott,David K Finlay,Emma E Vincent,Nicholas Jones,Annie M Curtis

Neural regeneration research 16:1813-1820 PubMed33510088

2021

Mitochonic acid 5 regulates mitofusin 2 to protect microglia.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Tan,Shuang-Xi Chen,Qing-Yun Lei,Shan-Qing Yi,Na Wu,Yi-Lin Wang,Zi-Jian Xiao,Heng Wu

Biochemical and biophysical research communication 525:733-739 PubMed32143825

2020

Cigarette smoke extract increases mitochondrial membrane permeability through activation of adenine nucleotide translocator (ANT) in lung epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kaiyue Wu,Guangxin Luan,Yanhong Xu,Shuang Shen,Shengnan Qian,Zhen Zhu,Xiaoying Zhang,Shaojun Yin,Jianping Ye

International journal of molecular medicine 45:151-161 PubMed31746366

2019

NR4A1 promotes TNF‑α‑induced chondrocyte death and migration injury via activating the AMPK/Drp1/mitochondrial fission pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhibo Zheng,Shuai Xiang,Yingjie Wang,Yulei Dong,Zeng Li,Yongbo Xiang,Yanyan Bian,Bin Feng,Bo Yang,Xisheng Weng

Cell stress & chaperones 24:991-1003 PubMed31388827

2019

Tanshinone IIA attenuates cardiac microvascular ischemia-reperfusion injury via regulating the SIRT1-PGC1α-mitochondrial apoptosis pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jiankai Zhong,Haichun Ouyang,Mingming Sun,Jianhua Lu,Yuanlin Zhong,Ying Tan,Yunzhao Hu

View all publications

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Product promise

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Complex II WB Antibody Cocktail

Western blot - Complex II WB Antibody Cocktail (AB110410)

Key facts

Applications

Reacts with

Form

Storage buffer

Reactivity data

Product details

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (39)

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