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AB140365

Membrane Fraction WB Cocktail

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(7 Publications)

Membrane Fraction WB Cocktail (ab140365) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
4 Images
Western blot - Membrane Fraction WB Cocktail (AB140365)
  • WB

Supplier Data

Western blot - Membrane Fraction WB Cocktail (AB140365)

Developed using ECL technique under reducing conditions. HeLa lysate prepared using the Membrane Fractionation Kit (ab139409). Exposure time : 5 mins. on a 4-20% gradient acrylamide gel, blocking and antibody incubation steps done in 5% milk, 20mM Tris-HCl, 0.1% TWEEN-20
Lane 1 : Marker
Lane 2 : HeLa Whole Cell Lysate
Lane 3 : HeLa Cytosolic Fraction Lysate
Lane 4 : HeLa Membrane Fraction Lysate
Lane 5 : HeLa Nuclear Fraction Lysate
Primary antibody : Membrane Fraction Western Blot cocktail
Secondary : HRP conjugated secondary antibody cocktail
Predicted/Observed band sizes :
Sodium Potassium ATPase : 112 kDa/100 kDa
GRP78 : 78 kDa/75 kDa
ATP5A : 60 kDa/60 kDa
GAPDH : 37 kDa/37kDa
Histone H3 (di methyl K9) : 17 kDa/17kDa
Notes : Percentage of antibody signal represents signal present in individual fractions as a proportion of the sum of all three fractions (cytoplasmic, membrane and nuclear)

false

Western blot - Membrane Fraction WB Cocktail (AB140365)
  • WB

Supplier Data

Western blot - Membrane Fraction WB Cocktail (AB140365)

Performed under reducing conditions. All blocking and antibody incubation steps were done in 5% milk in TBST. Developed using the ECL technique. Exposure time : 5 mins.

Sample 1 : Marker
Samples 2-5 : Hela Cell Lysate – 20 µg

Primary :
Lane 1 : none
Lane 2 : Anti- Sodium Potassium ATPase antibody – Plasma Membrane Marker
Lane 3 : Anti-GRP78 antibody – Endoplasmic Reticulum Marker
Lane 4 : Anti- ATP5a antibody – Mitochondrial Membrane Marker
Lane 5 : Anti- GAPDH antibody – Cytosolic Marker
Lane 6 : Anti- Histone H3 (di methyl K9) antibody – Nuclear Marker
Lane 7 : Assembled Membrane Antibody Cocktail

Secondary : HRP conjugated secondary antibody cocktail

Predicted/observed band sizes :
Sodium Potassium ATPase = 112 kDa/100 kDa
GRP78 band size : 78 kDa / 75 kDa
ATP5A band size : 60 kDa / 60 kDa
GAPDH band size : 37 kDa / 38 kDa
Histone H3 (di methyl K9) band size : 17 kDa / 17 kDa

false

Western blot - Membrane Fraction WB Cocktail (AB140365)
  • WB

Supplier Data

Western blot - Membrane Fraction WB Cocktail (AB140365)

Developed by IR scanning. HeLa cell lysate prepared using Kit ab139409. Exposure time : 5 mins . Samples run on a 4-20% gradient acrylamide gel. All blocking and antibody incubation steps were done in 5% milk, 20mM Tris-HCl, 0.1% TWEEN-20
Lane 1 : HeLa Whole Cell Lysate
Lane 2 : HeLa Cytosolic Fraction Lysate
Lane 3 : HeLa Membrane Fraction Lysate
Lane 4 : HeLa Nuclear Fraction Lysate
Primary antibody : Membrane Fraction Western Blot cocktail
Secondary : IR800 GAM (1/10000) and IR690 GAR (1/10000)
Predicted/Observed band sizes :
Sodium Potassium ATPase : 112 kDa/100 kDa
GRP78 : 78 kDa/75 kDa
ATP5A : 60 kDa/60 kDa
GAPDH : 37 kDa/37kDa
Histone H3 (di methyl K9) : 17 kDa/17kDa
Notes : Percentage of antibody signal (Y axis) represents the signal present in individual fractions as a proportion of the sum of all three fractions (cytoplasmic, membrane and nuclear)

false

Western blot - Membrane Fraction WB Cocktail (AB140365)
  • WB

Supplier Data

Western blot - Membrane Fraction WB Cocktail (AB140365)

Samples run on a 4-20% gradient acrylamide gel. Performed under reducing conditions. All blocking and antibody incubation steps were done in 5% milk, 20mM Tris-HCl, 0.1% TWEEN-20. Developed using the ECL technique. Exposure time : 5 mins.

Lane 1 : Marker
Lane 2 : Human heart homogenate Lysate – 20 µg
Lane 3 : Hela Cell Lysate – 20 µg
Lane 4 : Mouse heart homogenate Lysate - 20 µg
Lane 5 : NIH3T3 Cell Lysate – 20 µg
Lane 6 : Rat heart homogenate Lysate – 20 µg
Lane 7 : H9C2 Cell Lysate – 20 µg

Primary antibody : Membrane Fraction Western blot cocktail at 1/250 dilution

Secondary antibody : HRP conjugated secondary antibody cocktail at 1/2500 dilution

Predicted/observed band sizes :
Sodium Potassium ATPase = 112 kDa/100 kDa
GRP78 band size : 78 kDa / 75 kDa
ATP5A band size : 60 kDa / 60 kDa
GAPDH band size : 37 kDa / 38 kDa
Histone H3 (di methyl K9) band size : 17 kDa / 17 kDa

false

Key facts

Applications

WB

applications

Reacts with

Mouse, Rat, Human

Form

Liquid

form

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 0.9% Sodium chloride, 0.6% Tris-HCl buffer, 0.05% BSA

storage-buffer

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  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Primary antibody cocktail = 1/250 dilution. Secondary antibody cocktail = 1/2500 dilution</p>" } } }
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Product details

ab140365 contains 5 mAbs each targeting proteins located in different compartments of the cell. The presence of plasma membrane is shown by Anti-Sodium Potassium ATPase; endoplasmic reticulum is detected by GRP78; mitochondrial by ATP5A; cytosol by Anti-GAPDH; and nucleus by Anti-Histone H3 (di methyl K9).
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Product protocols

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Target data

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Nanomaterials (Basel, Switzerland) 14: PubMed38727378

2024

Towards a More Efficient Breast Cancer Therapy Using Active Human Cell Membrane-Coated Metal-Organic Frameworks.

Applications

Unspecified application

Species

Unspecified reactive species

Pablo Graván,Sara Rojas,Darina Francesca Picchi,Francisco Galisteo-González,Patricia Horcajada,Juan Antonio Marchal

ACS applied materials & interfaces 16:2058-2074 PubMed38159050

2024

Exploring the Impact of Nanoparticle Stealth Coatings in Cancer Models: From PEGylation to Cell Membrane-Coating Nanotechnology.

Applications

Unspecified application

Species

Unspecified reactive species

Pablo Graván,Jesús Peña-Martín,Julia López de Andrés,María Pedrosa,Martín Villegas-Montoya,Francisco Galisteo-González,Juan A Marchal,Paola Sánchez-Moreno

Cardiovascular diabetology 22:88 PubMed37072781

2023

Dysregulation of hypoxia-inducible factor 1α in the sympathetic nervous system accelerates diabetic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Petra Hrabalova,Romana Bohuslavova,Katerina Matejkova,Frantisek Papousek,David Sedmera,Pavel Abaffy,Frantisek Kolar,Gabriela Pavlinkova

Journal of neuroinflammation 18:63 PubMed33648543

2021

The psychoactive drug of abuse mephedrone differentially disrupts blood-brain barrier properties.

Applications

Unspecified application

Species

Unspecified reactive species

Tetyana P Buzhdygan,Cassidy R Rodrigues,Hannah M McGary,Jana A Khan,Allison M Andrews,Scott M Rawls,Servio H Ramirez

The Journal of clinical investigation 130:4999-5010 PubMed32584793

2020

12(S)-HETE mediates diabetes-induced endothelial dysfunction by activating intracellular endothelial cell TRPV1.

Applications

Unspecified application

Species

Unspecified reactive species

Mandy Otto,Clarissa Bucher,Wantao Liu,Melanie Müller,Tobias Schmidt,Marina Kardell,Marvin Noel Driessen,Jan Rossaint,Eric R Gross,Nana-Maria Wagner

The Journal of biological chemistry 290:23603-15 PubMed26260790

2015

Adipose Tissue Lipolysis Promotes Exercise-induced Cardiac Hypertrophy Involving the Lipokine C16:1n7-Palmitoleate.

Applications

WB

Species

Mouse

Anna Foryst-Ludwig,Michael C Kreissl,Verena Benz,Sarah Brix,Elia Smeir,Zsofia Ban,Elżbieta Januszewicz,Janek Salatzki,Jana Grune,Anne-Kathrin Schwanstecher,Annelie Blumrich,Andreas Schirbel,Robert Klopfleisch,Michael Rothe,Katharina Blume,Martin Halle,Bernd Wolfarth,Erin E Kershaw,Ulrich Kintscher

BMC endocrine disorders 14:11 PubMed24502509

2014

Partial deficiency of HIF-1α stimulates pathological cardiac changes in streptozotocin-induced diabetic mice.

Applications

WB

Species

Mouse

Romana Bohuslavova,Frantisek Kolar,David Sedmera,Lada Skvorova,Frantisek Papousek,Jan Neckar,Gabriela Pavlinkova
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Product promise

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