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Membrane Integrity WB Antibody Cocktail (ab110414) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.


Images

Western blot - Membrane Integrity WB Antibody Cocktail (AB110414), expandable thumbnail
  • Western blot - Membrane Integrity WB Antibody Cocktail (AB110414), expandable thumbnail

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Key facts

Applications
WB
Target
VDAC1
(See target data below)
Reactive species
Mouse, Rat, Cow, Human

Reactivity data

Application
WB
Reactivity
Reacts
Dilution info
-
Notes

The antibody cocktail (2.37 mg/mL) should be diluted to a final working concentration of 10.2 µg/mL for Western blotting.

Target data

Function

Non-selective voltage-gated ion channel that mediates the transport of anions and cations through the mitochondrion outer membrane and plasma membrane (PubMed:10661876, PubMed:11845315, PubMed:18755977, PubMed:30061676, PubMed:8420959). The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis (PubMed:10661876, PubMed:11845315, PubMed:18755977, PubMed:8420959). It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV (PubMed:10661876, PubMed:18755977, PubMed:8420959). The open state has a weak anion selectivity whereas the closed state is cation-selective (PubMed:18755977, PubMed:8420959). Binds various signaling molecules, including the sphingolipid ceramide, the phospholipid phosphatidylcholine, and the sterols cholesterol and oxysterol (PubMed:18755977, PubMed:31015432). In depolarized mitochondria, acts downstream of PRKN and PINK1 to promote mitophagy or prevent apoptosis; polyubiquitination by PRKN promotes mitophagy, while monoubiquitination by PRKN decreases mitochondrial calcium influx which ultimately inhibits apoptosis (PubMed:32047033). May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis (PubMed:15033708, PubMed:25296756). May mediate ATP export from cells (PubMed:30061676). Part of a complex composed of HSPA9, ITPR1 and VDAC1 that regulates mitochondrial calcium-dependent apoptosis by facilitating calcium transport from the ER lumen to the mitochondria intermembrane space thus providing calcium for the downstream calcium channel MCU that directly releases it into mitochondria matrix (By similarity). Mediates cytochrome c efflux (PubMed:20230784). Catalyzes the scrambling of phospholipids across the outer mitochondrial membrane; the mechanism is unrelated to channel activity and is capable of translocating both anionic and zwitterionic phospholipids.

Additional Targets

VDAC3, PPIF, UQCRC1, CYCS, ATP5F1A

Alternative names

VDAC, VDAC1, Non-selective voltage-gated ion channel VDAC1, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL, Porin 31HM, Voltage-dependent anion-selective channel protein 1, VDAC-1, hVDAC1

Recommended products

Membrane Integrity WB Antibody Cocktail (ab110414) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

Key facts

Applications
WB
Target
VDAC1
Reactive species
Mouse, Rat, Cow, Human

Storage

Shipped at conditions
Dry Ice

Notes

ab110414 (MS620) contains 5 mAbs against representative proteins for each of the primary structures of the mitochondrion:

Outer Membrane - Porin Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control ab14734 (MSA03)
Intermembrane Space - Cytochrome c Anti-Cytochrome C antibody [37BA11] ab110325 (MSA06)
Inner Membrane - Complex Va Anti-ATP5A antibody [7H10BD4F9] - Mitochondrial Marker ab110273 (MS502) and Complex III Core 1 Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] ab110252 (MS303)
Matrix Space - Cyclophilin D Anti-Cyclophilin F antibody [E11AE12BD4] ab110324 (MSA04)

This kit is useful in Western blotting to evaluate the structural integrity of isolated mitochondria. This kit is reactive against 39kDa human porin, 12kDa cytochrome c, 55kDa Complex Va, 49kDa Complex III Core 1, and 21kDa Cyclophilin D.

Cocktail Antibodies:

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2 product images

  • Western blot - Membrane Integrity WB Antibody Cocktail (ab110414), expandable thumbnail

    Western blot - Membrane Integrity WB Antibody Cocktail (ab110414)

    All lanes: Membrane Integrity WB Antibody Cocktail (ab110414)

    Lane 1: Rat liver mitos prep #1

    Lane 2: Rat liver mitos prep #2

    Lysates/proteins at 10 µg per lane

  • Western blot - Membrane Integrity WB Antibody Cocktail (ab110414), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Western blot - Membrane Integrity WB Antibody Cocktail (ab110414)

    Sample: Mouse whole cell lysate (3T3 SA cell line)
    Loading amount: 20μg
    Gel Running conditions: reduced denaturating (4-12% gradient gel)
    Blocking step: 30 min in 5% milk at room temperature
    Dilution: 1/1000
    Incubation time: Overnight at 4°C or 1 hour at room temperature
    Secondary antibody: Goat anti-mouse horseradish peroxidase linked, 1/5000 dilution
    Detection: ECL

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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