MitoBiogenesis™ Western Blot Cocktail (ab123545) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
Images
Publications
Filter by
- Frontiers in immunology 14:11482162023Metabolic reprogramming through mitochondrial biogenesis drives adenosine anti-inflammatory effects: new mechanism controlling gingival fibroblast hyper-inflammatory state.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNathalie Paladines,Shantiece Dawson,Weston Ryan,Rogelio Serrano-Lopez,Regina Messer,Yuqing Huo,Christopher W Cutler,Erivan S Ramos-Junior,Ana Carolina MorandiniPubMed 37350964
- Clinical cancer research : an official journal of the American Association for Cancer Research 28:1881-18952022Induction of Synthetic Lethality by Activation of Mitochondrial ClpP and Inhibition of HDAC1/2 in Glioblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTrang T T Nguyen,Enyuan Shang,Salveena Schiffgens,Consuelo Torrini,Chang Shu,Hasan Orhan Akman,Varun V Prabhu,Joshua E Allen,Mike-Andrew Westhoff,Georg Karpel-Massler,Markus D SiegelinPubMed 35417530
- ASN neuro 13:175909142110128882021Neuronal Pentraxin 1 Promotes Hypoxic-Ischemic Neuronal Injury by Impairing Mitochondrial Biogenesis via Interactions With Active Bax[6A7] and Mitochondrial Hexokinase II.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMd Al Rahim,Shabarish Thatipamula,Giulio M Pasinetti,Mir Ahamed HossainPubMed 34098747
- Cell chemical biology 28:1407-1419.e62021CYP27A1-dependent anti-melanoma activity of limonoid natural products targets mitochondrial metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyelim Cho,Qiong Shen,Lydia H Zhang,Mikiko Okumura,Akinori Kawakami,Jessi Ambrose,Frederic Sigoillot,Howard R Miller,Scott Gleim,Amanda Cobos-Correa,Ying Wang,Philippe Piechon,Guglielmo Roma,Fabian Eggimann,Charles Moore,Peter Aspesi,Felipa A Mapa,Heather Burks,Nathan T Ross,Philipp Krastel,Marc Hild,Thomas J Maimone,David E Fisher,Daniel K Nomura,John A Tallarico,Stephen M Canham,Jeremy L Jenkins,William C ForresterPubMed 33794192
- Cells 9:2020Inhibition of HDAC1/2 Along with TRAP1 Causes Synthetic Lethality in Glioblastoma Model Systems.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTrang T T Nguyen et. al.PubMed 32664214
- The Journal of clinical investigation 130:3699-37162020HDAC inhibitors elicit metabolic reprogramming by targeting super-enhancers in glioblastoma models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTrang Thi Thu Nguyen,Yiru Zhang,Enyuan Shang,Chang Shu,Consuelo Torrini,Junfei Zhao,Elena Bianchetti,Angeliki Mela,Nelson Humala,Aayushi Mahajan,Arif O Harmanci,Zhengdeng Lei,Mark Maienschein-Cline,Catarina M Quinzii,Mike-Andrew Westhoff,Georg Karpel-Massler,Jeffrey N Bruce,Peter Canoll,Markus D SiegelinPubMed 32315286
- European journal of human genetics : EJHG 27:1369-13782019A novel de novo MTOR gain-of-function variant in a patient with Smith-Kingsmore syndrome and Antiphospholipid syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaría Elena Rodríguez-García et. al.PubMed 31053780
- The Journal of biological chemistry 292:18556-185642017Glucose availability controls adipogenesis in mouse 3T3-L1 adipocytes via up-regulation of nicotinamide metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRobert M Jackson et. al.PubMed 28916720
- Diabetes 65:3585-35972016Enhanced GLUT4-Dependent Glucose Transport Relieves Nutrient Stress in Obese Mice Through Changes in Lipid and Amino Acid Metabolism.
- Cell stem cell 14:781-952014Pathways disrupted in human ALS motor neurons identified through genetic correction of mutant SOD1.
Key facts
- Applications
- WB
- Reactive species
- Mouse, Rat, Cow, Human
- Form
- Liquid
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Reacts | Dilution info - | Notes The antibody cocktail should be diluted 250 X to a final working concentration of 2µg/mL for western blotting. COXI is a highly hydrophobic protein and appears as a broad band at ~35 kDa (not at its true molecular weight at 57 kDa). It is very sensitive to heating. Therefore, the samples, including the positive control, should not be heated over 50°C before loaded on the gel. |
Recommended products
MitoBiogenesis™ Western Blot Cocktail (ab123545) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
Key facts
- Applications
- WB
- Reactive species
- Mouse, Rat, Cow, Human
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
The MitoBiogenesis™ western blotting cocktail (ab123545) is designed to study the regulation of mitochondrial biogenesis and cellular stress in response to environmental stimuli. It also can be used to monitor drug-induced effects on mitochondrial biogenesis. The two main components of this cocktail target two proteins, which are each subunits of a different oxidative phosphorylation enzyme complex, one subunit I of Complex IV (COX-I), which is mtDNA-encoded, and the 70kDa subunit of Complex II (SDH-A), which is nDNA-encoded. Complex IV includes several proteins which are encoded in the mitochondrion, while the proteins of Complex II are entirely encoded in the nucleus. An anti-Beta actin antibody is used as loading control.
Cocktail Antibodies:
Mouse CII-70 (SDHA) [2E3GC12FB2AE2] monoclonal:
Amount: 25 μg
Working concentration: 0.5 μg/ml
Mouse CIV-1 (MT-CO1) [1D6E1A8] monoclonal:
Amount: 50 μg
Working concentration: 1 μg/ml
Mouse Beta actin [mAbcam 8224] monoclonal:
Amount: 25 μg
Working concentration: 0.5 μg/ml
**Antibody Purity:** Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.**Related products**Review the , or the full to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The combined target of SDHA COX1 and Beta-Actin involves three proteins with distinct mechanical roles in the cell. SDHA also known as succinate dehydrogenase subunit A exhibits a mass of approximately 70 kDa and functions as part of the mitochondrial complex II. COX1 or cytochrome c oxidase subunit 1 weighs around 57 kDa and is integral to the electron transport chain. Beta-Actin a cytoskeletal protein with a mass of about 42 kDa maintains cellular structure. SDHA and COX1 are expressed in mitochondria whereas Beta-Actin is found in the cytoplasm of most eukaryotic cells.
- Biological function summary
SDHA helps convert succinate to fumarate in the Krebs cycle also known as the citric acid cycle as part of mitochondrial complex II. COX1 facilitates the transfer of electrons from cytochrome c to oxygen in mitochondrial complex IV. Beta-Actin plays roles in maintaining cell shape enabling cell motility and facilitating intracellular transport. Together SDHA and COX1 are involved in mitochondrial activities essential for energy production while Beta-Actin supports structural integrity.
- Pathways
SDHA and COX1 significantly contribute to oxidative phosphorylation and the Krebs cycle important for energy metabolism. SDHA interacts with fumarate hydratase and succinate-coenzyme Q reductase in energy production pathways whereas COX1 works synergistically with other cytochrome c oxidase subunits. Beta-Actin is less involved in metabolic pathways and more associated with pathways governing cell movement and division interacting with proteins like myosin and tropomyosin.
- Associated diseases and disorders
SDHA and COX1 are implicated in mitochondrial dysfunctions such as Leigh syndrome and mitochondrial complex II deficiency. These disorders often lead to compromised energy metabolism and neurological symptoms. Beta-Actin abnormalities might relate to cancer metastasis due to its role in cell movement and structure. Disrupted interactions between these proteins and their pathways can result in altered cellular energy production and structural organization exacerbating disease conditions.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - MitoBiogenesis™ Western Blot Cocktail (ab123545)
Western blot - MitoBiogenesis™ Western Blot Cocktail (ab123545)
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com