AB123545

MitoBiogenesis™ Western Blot Cocktail

Name: MitoBiogenesis™ Western Blot Cocktail (ab123545) | Abcam
Brand: Abcam Limited
SKU: AB123545
Rating: 1 (1 reviews)

(1 Review)

(11 Publications)

MitoBiogenesis™ Western Blot Cocktail (ab123545) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

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Supplier Data

Western blot - MitoBiogenesis™ Western Blot Cocktail (AB123545)

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Supplier Data

Western blot - MitoBiogenesis™ Western Blot Cocktail (AB123545)

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Key facts

Applications

applications

Reacts with

Mouse, Rat, Cow, Human

Form

Liquid

form

Storage buffer

Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>The antibody cocktail should be diluted 250 X to a final working concentration of 2µg/mL for western blotting. COXI is a highly hydrophobic protein and appears as a broad band at ~35 kDa (not at its true molecular weight at 57 kDa). It is very sensitive to heating. Therefore, the samples, including the positive control, should not be heated over 50°C before loaded on the gel.</p>" } } }

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Product details

The MitoBiogenesis™ western blotting cocktail (ab123545) is designed to study the regulation of mitochondrial biogenesis and cellular stress in response to environmental stimuli. It also can be used to monitor drug-induced effects on mitochondrial biogenesis. The two main components of this cocktail target two proteins, which are each subunits of a different oxidative phosphorylation enzyme complex, one subunit I of Complex IV (COX-I), which is mtDNA-encoded, and the 70kDa subunit of Complex II (SDH-A), which is nDNA-encoded. Complex IV includes several proteins which are encoded in the mitochondrion, while the proteins of Complex II are entirely encoded in the nucleus. An anti-Beta actin antibody is used as loading control.

Cocktail Antibodies:

Mouse CII-70 (SDHA) [2E3GC12FB2AE2] monoclonal:

Amount: 25 μg

Working concentration: 0.5 μg/ml

Mouse CIV-1 (MT-CO1) [1D6E1A8] monoclonal:

Amount: 50 μg

Working concentration: 1 μg/ml

Mouse Beta actin [mAbcam 8224] monoclonal:

Amount: 25 μg

Working concentration: 0.5 μg/ml

**Antibody Purity:** Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.**Related products**Review the , or the full to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The combined target of SDHA COX1 and Beta-Actin involves three proteins with distinct mechanical roles in the cell. SDHA also known as succinate dehydrogenase subunit A exhibits a mass of approximately 70 kDa and functions as part of the mitochondrial complex II. COX1 or cytochrome c oxidase subunit 1 weighs around 57 kDa and is integral to the electron transport chain. Beta-Actin a cytoskeletal protein with a mass of about 42 kDa maintains cellular structure. SDHA and COX1 are expressed in mitochondria whereas Beta-Actin is found in the cytoplasm of most eukaryotic cells.

Biological function summary

SDHA helps convert succinate to fumarate in the Krebs cycle also known as the citric acid cycle as part of mitochondrial complex II. COX1 facilitates the transfer of electrons from cytochrome c to oxygen in mitochondrial complex IV. Beta-Actin plays roles in maintaining cell shape enabling cell motility and facilitating intracellular transport. Together SDHA and COX1 are involved in mitochondrial activities essential for energy production while Beta-Actin supports structural integrity.

Pathways

SDHA and COX1 significantly contribute to oxidative phosphorylation and the Krebs cycle important for energy metabolism. SDHA interacts with fumarate hydratase and succinate-coenzyme Q reductase in energy production pathways whereas COX1 works synergistically with other cytochrome c oxidase subunits. Beta-Actin is less involved in metabolic pathways and more associated with pathways governing cell movement and division interacting with proteins like myosin and tropomyosin.

SDHA and COX1 are implicated in mitochondrial dysfunctions such as Leigh syndrome and mitochondrial complex II deficiency. These disorders often lead to compromised energy metabolism and neurological symptoms. Beta-Actin abnormalities might relate to cancer metastasis due to its role in cell movement and structure. Disrupted interactions between these proteins and their pathways can result in altered cellular energy production and structural organization exacerbating disease conditions.

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1148216 PubMed37350964

2023

Metabolic reprogramming through mitochondrial biogenesis drives adenosine anti-inflammatory effects: new mechanism controlling gingival fibroblast hyper-inflammatory state.

Applications

Unspecified application

Species

Unspecified reactive species

Nathalie Paladines,Shantiece Dawson,Weston Ryan,Rogelio Serrano-Lopez,Regina Messer,Yuqing Huo,Christopher W Cutler,Erivan S Ramos-Junior,Ana Carolina Morandini

Clinical cancer research : an official journal of the American Association for Cancer Research 28:1881-1895 PubMed35417530

2022

Induction of Synthetic Lethality by Activation of Mitochondrial ClpP and Inhibition of HDAC1/2 in Glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Trang T T Nguyen,Enyuan Shang,Salveena Schiffgens,Consuelo Torrini,Chang Shu,Hasan Orhan Akman,Varun V Prabhu,Joshua E Allen,Mike-Andrew Westhoff,Georg Karpel-Massler,Markus D Siegelin

ASN neuro 13:17590914211012888 PubMed34098747

2021

Neuronal Pentraxin 1 Promotes Hypoxic-Ischemic Neuronal Injury by Impairing Mitochondrial Biogenesis via Interactions With Active Bax[6A7] and Mitochondrial Hexokinase II.

Applications

Unspecified application

Species

Unspecified reactive species

Md Al Rahim,Shabarish Thatipamula,Giulio M Pasinetti,Mir Ahamed Hossain

Cell chemical biology 28:1407-1419.e6 PubMed33794192

2021

CYP27A1-dependent anti-melanoma activity of limonoid natural products targets mitochondrial metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Hyelim Cho,Qiong Shen,Lydia H Zhang,Mikiko Okumura,Akinori Kawakami,Jessi Ambrose,Frederic Sigoillot,Howard R Miller,Scott Gleim,Amanda Cobos-Correa,Ying Wang,Philippe Piechon,Guglielmo Roma,Fabian Eggimann,Charles Moore,Peter Aspesi,Felipa A Mapa,Heather Burks,Nathan T Ross,Philipp Krastel,Marc Hild,Thomas J Maimone,David E Fisher,Daniel K Nomura,John A Tallarico,Stephen M Canham,Jeremy L Jenkins,William C Forrester

Cells 9: PubMed32664214

2020

Inhibition of HDAC1/2 Along with TRAP1 Causes Synthetic Lethality in Glioblastoma Model Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Trang T T Nguyen,Yiru Zhang,Enyuan Shang,Chang Shu,Catarina M Quinzii,Mike-Andrew Westhoff,Georg Karpel-Massler,Markus D Siegelin

The Journal of clinical investigation 130:3699-3716 PubMed32315286

2020

HDAC inhibitors elicit metabolic reprogramming by targeting super-enhancers in glioblastoma models.

Applications

Unspecified application

Species

Unspecified reactive species

Trang Thi Thu Nguyen,Yiru Zhang,Enyuan Shang,Chang Shu,Consuelo Torrini,Junfei Zhao,Elena Bianchetti,Angeliki Mela,Nelson Humala,Aayushi Mahajan,Arif O Harmanci,Zhengdeng Lei,Mark Maienschein-Cline,Catarina M Quinzii,Mike-Andrew Westhoff,Georg Karpel-Massler,Jeffrey N Bruce,Peter Canoll,Markus D Siegelin

European journal of human genetics : EJHG 27:1369-1378 PubMed31053780

2019

A novel de novo MTOR gain-of-function variant in a patient with Smith-Kingsmore syndrome and Antiphospholipid syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

María Elena Rodríguez-García,Francisco Javier Cotrina-Vinagre,Marcello Bellusci,Ana Martínez de Aragón,Laura Hernández-Sánchez,Patricia Carnicero-Rodríguez,Elena Martín-Hernández,Francisco Martínez-Azorín

The Journal of biological chemistry 292:18556-18564 PubMed28916720

2017

Glucose availability controls adipogenesis in mouse 3T3-L1 adipocytes via up-regulation of nicotinamide metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Robert M Jackson,Beth A Griesel,Jami M Gurley,Luke I Szweda,Ann Louise Olson

Diabetes 65:3585-3597 PubMed27679559

2016

Enhanced GLUT4-Dependent Glucose Transport Relieves Nutrient Stress in Obese Mice Through Changes in Lipid and Amino Acid Metabolism.

Applications

Species

Mouse

Jami M Gurley,Olga Ilkayeva,Robert M Jackson,Beth A Griesel,Phillip White,Satochi Matsuzaki,Rizwan Qaisar,Holly Van Remmen,Kenneth M Humphries,Christopher B Newgard,Ann Louise Olson

Cell stem cell 14:781-95 PubMed24704492

2014

Pathways disrupted in human ALS motor neurons identified through genetic correction of mutant SOD1.

Applications

Species

Human

Evangelos Kiskinis,Jackson Sandoe,Luis A Williams,Gabriella L Boulting,Rob Moccia,Brian J Wainger,Steve Han,Theodore Peng,Sebastian Thams,Shravani Mikkilineni,Cassidy Mellin,Florian T Merkle,Brandi N Davis-Dusenbery,Michael Ziller,Derek Oakley,Justin Ichida,Stefania Di Costanzo,Nick Atwater,Morgan L Maeder,Mathew J Goodwin,James Nemesh,Robert E Handsaker,Daniel Paull,Scott Noggle,Steven A McCarroll,J Keith Joung,Clifford J Woolf,Robert H Brown,Kevin Eggan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

MitoBiogenesis™ Western Blot Cocktail

Western blot - MitoBiogenesis™ Western Blot Cocktail (AB123545)

Western blot - MitoBiogenesis™ Western Blot Cocktail (AB123545)

Key facts

Applications

Reacts with

Form

Storage buffer

Reactivity data

Product details

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

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