Plasma Membrane Fraction Western Blot Cocktail
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(2 Publications)
- WB
Supplier Data
Western blot - Plasma Membrane Fraction Western Blot Cocktail (AB139413)
Developed using the ECL technique; Performed under reducing conditions; Exposure time : 5 mins; All blocking and antibody incubation steps were done in 5% milk, 20 mM Tris-HCl, 0.1% TWEEN-20
Lane 1 : Marker
Lanes 2-5 : HeLa Whole Cell Lysate – 20 µg
Primary antibodies :
Lane 1 : none
Lane 2 : Anti- Sodium ATPase antibody – Plasma Membrane Marker
Lane 3 : Anti- GAPDH antibody – Cytosolic Membrane Marker
Lane 4 : Anti-Histone 3 antibody – Nuclear Membrane Marker
Lane 5 : Assembled Plasma Membrane Antibody Cocktail
Secondary : ab131368 at 1/1000 dilution
Predicted Sodium Potassium ATPase band size : 112 kDa
Observed Sodium Potassium ATPase band size : 100 kDa
Predicted GAPDH band size : 37 kDa
Observed GAPDH band size : 37 kDa
Predicted Histone 3 band size : 17 kDa
Observed Histone 3 band size : 17 kD
false
- WB
Supplier Data
Western blot - Plasma Membrane Fraction Western Blot Cocktail (AB139413)
Developed using ECL technique under reducing conditions; exposure time 3 mins; blocking and antibody incubation steps done in 5% milk/TBST
Lanes :
1 : Marker
2 : Human heart homogenate Tissue Lysate – 20 µg
3 : HeLa Cell Lysate – 20 µg
4 : Mouse heart homogenate Tissue Lysate - 20 µg
5 : NIH3T3 Cell Lysate – 20 µg
6 : Rat heart homogenate Tissue Lysate – 20 µg
7 : H9C2 cell lysate – 20 µg
All Lanes :
Anti-Sodium ATPase antibody – Plasma Membrane Marker
Anti-GAPDH antibody – Cytosolic Marker
Anti-Histone H3 (di methyl k9) antibody – Nuclear Marker
Secondary : ab131368 at 1/1000 dilution
Predicted Sodium Potassium ATPase band size : 112 kDa
Observed Sodium Potassium ATPase band size : 100 kDa
Predicted GAPDH band size : 37 kDa
Observed GAPDH band size : 37 kDa
Predicted Histone H3 (di methyl k9) band size : 17 kDa
Observed Histone H3 (di methyl k9 band size : 17 kDa
false
- WB
Supplier Data
Western blot - Plasma Membrane Fraction Western Blot Cocktail (AB139413)
Developed using ECL technique, reducing conditions, exposured 5 mins
Blocking and antibody incubations done in 5% milk, 20 mM Tris-HCl, 0.1% TWEEN-20
Lanes :
1 : Marker
2 : HeLa Whole Cell Lysate - 20 µl
3 : HeLa Cytosolic Fraction Lysate - 20 µl
4 : HeLa Membrane Fraction Lysate - 20 µl
5 : HeLa Nuclear Fraction Lysate - 20 µl
All Lanes :
Anti-Sodium ATPase antibody – Plasma Membrane Marker
Anti-GAPDH antibody – Cytosolic Membrane Marker
Anti-Histone 3 antibody – Nuclear Membrane Marker
Secondary :
Goat polyclonal to Mouse IgG (ab6789) – H&L (HRP)
Predicted Sodium Potassium ATPase band size : 112 kDa
Observed Sodium Potassium ATPase band size : 100 kDa
Predicted GAPDH band size : 37 kDa
Observed GAPDH band size : 37 kDa
Predicted Histone 3 band size : 17 kDa
Observed Histone 3 band size : 17 kDa
false
Reactivity data
Product details
ab139413 contains 3 mouse monoclonal antibodies each targeting a specific organelle marker. The presence of plasma membrane is determined by Anti-Sodium Potassium ATPase antibody; cytosol by Anti-GAPDH; and nucleus by Anti-Histone H3 (di methyl K9). This cocktail is suitable for determining the purity of organelle isolates prior to further characterization.
This product is particularly valuable to researchers working in organelle proteomics. Mass spectrometry is frequently used in this field to determine the protein content of targeted organelle isolates. These isolates are obtained using differential centrifugation, density gradient fractionation, biochemical enrichment, or affinity purification. Unfortunately, the various methods of purification available for organelle isolation are imperfect and leave behind contaminants from undesired regions of the cell. These contaminants are inevitable, but being aware of which contaminants are present is crucial for analysis of mass spectrometry results. The high sensitivity and species cross reactivity of the antibodies in this cocktail will quickly and easily reveal impurities caused by imperfect sample preparation.
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This ATPase plays a central role in cellular osmotic balance and volume regulation. It operates as a part of a larger enzyme complex that interacts with other proteins to ensure proper cell function. Besides its key involvement in ion transport this enzyme influences cellular processes like signal transduction and nutrient uptake by modulating membrane potential and cell responses.
Pathways
Sodium Potassium ATPase is integrated into vital signaling pathways that include the MAPK/ERK and PKA pathways. Through these pathways it interacts with proteins such as growth factors and neurotransmitter receptors impacting cell proliferation and communication. This integration highlights its importance in maintaining cellular homeostasis and facilitating intercellular communication.
Target data
Publications (2)
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Journal of experimental & clinical cancer research 40:20 PubMed33413577
2021
Applications
Unspecified application
Species
Unspecified reactive species
Journal of proteomics 187:126-143 PubMed30012418
2018
Applications
Unspecified application
Species
Unspecified reactive species
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