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AB184868

PRX Pathway (TRX, TXNRD1, PRX1) Western Blot Cocktail

4

(1 Review)

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(5 Publications)

PRX Pathway (TRX, TXNRD1, PRX1) Western Blot Cocktail (ab184868) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
3 Images
Western blot - PRX Pathway (TRX, TXNRD1, PRX1) Western Blot Cocktail (AB184868)
  • WB

Supplier Data

Western blot - PRX Pathway (TRX, TXNRD1, PRX1) Western Blot Cocktail (AB184868)

Densiometric analysis of a Western blot obtained with the PRX Pathway WB cocktail using various cell lines to determine the relative amounts of thioredoxin reductase 1, peroxiredoxin 1, and thioredoxin.

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Western blot - PRX Pathway (TRX, TXNRD1, PRX1) Western Blot Cocktail (AB184868)
  • WB

Supplier Data

Western blot - PRX Pathway (TRX, TXNRD1, PRX1) Western Blot Cocktail (AB184868)

WB lysate sample was heated at 95°C for 10 minutes before loading. Performed under reducing conditions. All blocking and antibody incubation steps were done in 5% milk in TBST. Developed using the ECL technique.

Exposure time : 1 minute.

Sample : HeLa Cell Lysate – 20 μg/lane

Lane 1 : Anti-Thioredoxin Reductase 1 antibody

Lane 2 : Anti-Peroxiredoxin 1 antibody

Lane 3 : Anti-Thioredoxin antibody

Lane 4 : PRX Pathway WB Cocktail

Secondary : HRP-conjugated Anti-Rabbit IgG

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Western blot - PRX Pathway (TRX, TXNRD1, PRX1) Western Blot Cocktail (AB184868)
  • WB

Supplier Data

Western blot - PRX Pathway (TRX, TXNRD1, PRX1) Western Blot Cocktail (AB184868)

The PRX Pathway was used on various cell types to determine the relative amounts of thioredoxin reductase 1, peroxiredoxin 1, and thioredoxin.

All blocking and antibody incubation steps were done in 5% milk in TBST. Developed using the ECL technique.

Exposure time : 1 minute.

20 μg of each cell lysate was loaded per lane after heating for 10 minutes at 95°C.

Lane 1 : Hela

Lane 2 : HepG2

Lane 3 : HDFn

Lane 4 : Hek293T

Lane 5 : MCF7

Lane 6 : Jurkat

Lane 7 : HL60

Lane 8 : H9C2

Lane 9 : MEF

Secondary : HRP-conjugated Anti-Rabbit IgG

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Key facts

Applications

WB

applications

Reacts with

Mouse, Rat, Human

Form

Liquid

form

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 0.66% Sodium chloride, 0.61% Tris, 0.5% Glycerol (glycerin, glycerine), 0.05% BSA

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Primary Antibodies

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Primary Antibodies

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  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>WB samples should be heated to 95°C for 10 minutes in sample buffer containing DTT before loading. Suggested working concentration is 1X for the antibody cocktail. Suggested incubation buffer is 5% milk in TBS + 0.05%Tween 20. Optimized for cell lysates, may also work on tissue homogenates. Expected and observed MWs: Thioredoxin: 12 kDa, Thioredoxin Reductase 1: 55 kDa, Peroxiredoxin 1: 22 kDa.</p>" } } }
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Product details

The PRX pathway Western blot cocktail is designed to determine the relative abundance of several important proteins involved in the thioredoxin redox pathway.

Thioredoxin (TRX) is a small enzyme (12kDa) that facilitates the reduction of other enzymes via cysteine thiol-disulfide exchange. Thioredoxin is used by cells to reduce ROS amounts and in redox signaling processes. Thioredoxin reductase 1 (TXNRD1) is found in the cytoplasm and possesses glutaredoxin and thioredoxin reductase activity. TXNRD1 utilizes its redox-active disulfide bond to reduce oxidized thioredoxin. Also involved in the thioredoxin redox pathway and additionally shown to be upregulated in a variety of cancers is the relatively abundant peroxiredoxin 1 (PRX1). Peroxiredoxin 1 is an antioxidant enzyme that helps regulate peroxide levels and is involved in redox signaling. Peroxiredoxin 1's activity is regenerated after being reduced by thioredoxin.

These three readouts are easily resolved by Western blot given their different molecular weights. As all primary antibodies in the WB cocktail are rabbit antibodies, an anti-rabbit secondary should be used for detection.

Expected and observed MWs:
Thioredoxin: 12 kDa
Thioredoxin Reductase 1: 55 kDa
Peroxiredoxin 1: 22 kDa

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRX+TXNRD1+PRX1 refers to the thioredoxin (TRX) thioredoxin reductase 1 (TXNRD1) and peroxiredoxin 1 (PRX1) system significant players in the regulation of redox homeostasis. TRX also known as thioredoxin-1 has a molecular weight of approximately 12 kDa and is present in most mammalian tissues. TXNRD1 is part of the selenoprotein family with a mass of around 55 kDa and expresses widely in cells including those in the liver lungs and kidneys. PRX1 is about 22 kDa in weight and is mainly localized in the cytosol. Together this system operates predominantly in the cytoplasm but can also function within the mitochondria.
Biological function summary

The TRX+TXNRD1+PRX1 system serves as a central antioxidant defense and is involved in cellular response to oxidative stress. This trio does not function individually but works as an integrated complex maintaining a balanced redox state in cells. Thioredoxin acts as an electron donor to peroxiredoxin reducing hydrogen peroxide to water a process important to remove harmful reactive oxygen species. Thioredoxin reductase then regenerates the reduced form of thioredoxin sustaining this antioxidant cycle and additionally influencing transcription factors that regulate cell survival and apoptosis.

Pathways

Thioredoxin thioredoxin reductase and peroxiredoxin operate in key pathways like the NADPH-dependent reduction pathway and MAPK signaling pathway. The TRX system utilizes NADPH to reduce thioredoxin integral to protecting against oxidative damage. The pathway relates to other significant proteins like glutathione another cellular antioxidant that collaborates with the TRX system in defending cells from oxidative stress. In addition the MAPK pathway involves thioredoxin participating in signal transduction mechanisms that affect cell proliferation and response to stress.

The TRX+TXNRD1+PRX1 system links closely to cancer and cardiovascular diseases. Its antioxidant properties contribute to the regulation of cell proliferation and prevention of apoptosis processes that when malfunctioning may lead to tumorigenesis and progression of cancer. The system's malfunction can also contribute to oxidative stress promoting atherosclerosis an important factor in cardiovascular diseases. The protein family related to these diseases includes nuclear factor-kappa B (NF-κB) a transcription factor that the TRX system regulates affecting inflammation and cell survival pathways relevant to disease progression.
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Product protocols

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Target data

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Science advances 8:eabl8920 PubMed35108055

2022

Dexamethasone sensitizes to ferroptosis by glucocorticoid receptor-induced dipeptidase-1 expression and glutathione depletion.

Applications

Unspecified application

Species

Unspecified reactive species

Anne von Mässenhausen,Nadia Zamora Gonzalez,Francesca Maremonti,Alexia Belavgeni,Wulf Tonnus,Claudia Meyer,Kristina Beer,Monica T Hannani,Arthur Lau,Mirko Peitzsch,Paul Hoppenz,Sophie Locke,Triantafyllos Chavakis,Rafael Kramann,Daniel A Muruve,Christian Hugo,Stefan R Bornstein,Andreas Linkermann

Antioxidants (Basel, Switzerland) 10: PubMed33673502

2021

Altered Expression of Peroxiredoxins in Mouse Model of Progressive Myoclonus Epilepsy upon LPS-Induced Neuroinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Mojca Trstenjak Prebanda,Petra Matjan-Štefin,Boris Turk,Nataša Kopitar-Jerala

Cells 8: PubMed31766320

2019

Upregulation of Mitochondrial Redox Sensitive Proteins in LPS-Treated Stefin B-Deficient Macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Mojca Trstenjak Prebanda,Janja Završnik,Boris Turk,Nataša Kopitar Jerala

PloS one 12:e0188421 PubMed29161316

2017

Acute exercise alters skeletal muscle mitochondrial respiration and H2O2 emission in response to hyperinsulinemic-euglycemic clamp in middle-aged obese men.

Applications

Unspecified application

Species

Unspecified reactive species

Adam J Trewin,Itamar Levinger,Lewan Parker,Christopher S Shaw,Fabio R Serpiello,Mitchell J Anderson,Glenn K McConell,David L Hare,Nigel K Stepto

American journal of physiology. Heart and circulatory physiology 308:H291-302 PubMed25485897

2014

Restoring redox balance enhances contractility in heart trabeculae from type 2 diabetic rats exposed to high glucose.

Applications

WB

Species

Rat

Niraj M Bhatt,Miguel A Aon,Carlo G Tocchetti,Xiaoxu Shen,Swati Dey,Genaro Ramirez-Correa,Brian O'Rourke,Wei Dong Gao,Sonia Cortassa
View all publications
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