Pyruvate dehydrogenase (PDH) WB Antibody Cocktail (ab110416) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
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- Biomedicines 10:2022Identification of Novel Mitochondrial Pyruvate Carrier Inhibitors by Homology Modeling and Pharmacophore-Based Virtual Screening.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLamees Hegazy,Lauren E Gill,Kelly D Pyles,Christopher Kaiho,Sophia Kchouk,Brian N Finck,Kyle S McCommis,Bahaa ElgendyPubMed 35203575
- Cancers 13:2021Non-Canonical Role of PDK1 as a Negative Regulator of Apoptosis through Macromolecular Complexes Assembly at the ER-Mitochondria Interface in Oncogene-Driven NSCLC.Applications:Unspecified applicationReactive species:Unspecified reactive speciesViviana De Rosa,Francesca Iommelli,Cristina Terlizzi,Eleonora Leggiero,Rosa Camerlingo,Giovanna G Altobelli,Rosa Fonti,Lucio Pastore,Silvana Del VecchioPubMed 34439291
- Journal of orthopaedic research : official publication of the Orthopaedic Research Society 40:1026-10382021MuRF1 deficiency prevents age-related fat weight gain, possibly through accumulation of PDK4 in skeletal muscle mitochondria in older mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKosuke Sugiura,Katsuya Hirasaka,Tasuku Maeda,Takayuki Uchida,Koji Kishimoto,Motoko Oarada,Siegfried Labeit,Anayt Ulla,Iori Sakakibara,Reiko Nakao,Koichi Sairyo,Takeshi NikawaPubMed 34185335
- JCI insight 6:2021Myocardial Lipin 1 knockout in mice approximates cardiac effects of human LPIN1 mutations.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKari T Chambers,Michael A Cooper,Alison R Swearingen,Rita T Brookheart,George G Schweitzer,Carla J Weinheimer,Attila Kovacs,Timothy R Koves,Deborah M Muoio,Kyle S McCommis,Brian N FinckPubMed 33986192
- Redox biology 36:1016602020Pleiotropic effects of mdivi-1 in altering mitochondrial dynamics, respiration, and autophagy in cardiomyocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRicha Aishwarya et. al.PubMed 32750667
- Journal of clinical medicine 9:2020Beneficial Effects of Acetyl-DL-Leucine (ADLL) in a Mouse Model of Sandhoff Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEcem Kaya et. al.PubMed 32276303
- Disease models & mechanisms 12:2019Deterioration of mitochondrial bioenergetics and ultrastructure impairment in skeletal muscle of a transgenic minipig model in the early stages of Huntington's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarie Rodinova et. al.PubMed 31278192
- Scientific reports 9:20022019Doxorubicin-induced cardiomyopathy associated with inhibition of autophagic degradation process and defects in mitochondrial respiration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChowdhury S Abdullah et. al.PubMed 30765730
- Cancer & metabolism 7:12019The novel function of tumor protein D54 in regulating pyruvate dehydrogenase and metformin cytotoxicity in breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYongxian Zhuang,Reynold C Ly,Carleigh V Frazier,Jia Yu,Sisi Qin,Xiao-Yang Fan,Matthew P Goetz,Judy C Boughey,Richard Weinshilboum,Liewei WangPubMed 30697423
- Journal of the American Heart Association 7:e0097752018Cardiac Dysfunction in the Sigma 1 Receptor Knockout Mouse Associated With Impaired Mitochondrial Dynamics and Bioenergetics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChowdhury S Abdullah,Shafiul Alam,Richa Aishwarya,Sumitra Miriyala,Manikandan Panchatcharam,Mohammad Alfrad Nobel Bhuiyan,Jonette M Peretik,A Wayne Orr,Jeanne James,Hanna Osinska,Jeffrey Robbins,John N Lorenz,Md Shenuarin BhuiyanPubMed 30371279
Key facts
- Applications
- WB
- Reactive species
- Cow, Human
- Form
- Liquid
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Reacts | Dilution info - | Notes Suggested concentration. |
Recommended products
Pyruvate dehydrogenase (PDH) WB Antibody Cocktail (ab110416) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
Key facts
- Applications
- WB
- Reactive species
- Cow, Human
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 0.15% HEPES
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab110416 (MSP02) contains a total of 150 µg of monoclonal antibodies reactive against PDH subunits. Isolated mitochondria are available from several species for use as control samples.
The PDH Western blotting kit contains four different mAbs reacting specifically with E1 alpha, E1 beta, E2 and E2/E3bp. A mAb against the OSCP subunit of ATP synthase is included as a control. This mAb mixture can be used to probe the isolated pyruvate dehydrogenase for integrity and purity.
Cocktail Antibodies:
Mouse anti C-V-OSCP [4C11C10D12] monoclonal:
Amount: 12.5 µg
Working concentration: 0.5 µg/ml
Mouse anti PDH-E1-alpha [9H9AF5] monoclonal:
Amount: 25 µg
Working concentration: 1 µg/ml
Mouse anti PDH-E1-beta [17A5E2H8] monoclonal:
Amount: 62.5 µg
Working concentration: 2.5 µg/ml
Mouse anti PDH-E2 [15D3G9C11] monoclonal:
Amount: 25 µg
Working concentration: 1 µg/ml
Mouse anti PDH-E2/E3bp [13G2AE2BH5] monoclonal:
Amount: 25 µg
Working concentration: 1 µg/ml
Product promise
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Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Western blot - Pyruvate dehydrogenase (PDH) WB Antibody Cocktail (ab110416)
Blocked for overnight with 5% Milk/PBS at 4°C (or 3 hrs at room temperature).
Primary antibody incubatedat 6 μg/mL in 1% milk/PBS for 2hrs at room temperature.
Secondary: HRP-conjugated anti-mouse used according to manufacturers instructions.
Detection method: ECL+
All wash steps carried out in PBS + 0.05% Tween-20.
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