AB110416

Pyruvate dehydrogenase (PDH) WB Antibody Cocktail

Name: Pyruvate dehydrogenase (PDH) WB Antibody Cocktail (ab110416) | Abcam
Brand: Abcam Limited
SKU: AB110416
Price: 500 USD
Availability: InStock

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(35 Publications)

Pyruvate dehydrogenase (PDH) WB Antibody Cocktail (ab110416) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

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Supplier Data

Western blot - Pyruvate dehydrogenase (PDH) WB Antibody Cocktail (AB110416)

Blocked for overnight with 5% Milk/PBS at 4°C (or 3 hrs at room temperature).

Primary antibody incubatedat 6 μg/mL in 1% milk/PBS for 2hrs at room temperature.

Secondary : HRP-conjugated anti-mouse used according to manufacturers instructions.

Detection method : ECL+

All wash steps carried out in PBS + 0.05% Tween-20.

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Key facts

Applications

applications

Reacts with

Cow, Human

Form

Liquid

form

Storage buffer

Preservative: 0.02% Sodium azide Constituents: 0.15% HEPES

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Suggested concentration.</p>" } } }

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Product details

ab110416 (MSP02) contains a total of 150 µg of monoclonal antibodies reactive against PDH subunits. Isolated mitochondria are available from several species for use as control samples.

The PDH Western blotting kit contains four different mAbs reacting specifically with E1 alpha, E1 beta, E2 and E2/E3bp. A mAb against the OSCP subunit of ATP synthase is included as a control. This mAb mixture can be used to probe the isolated pyruvate dehydrogenase for integrity and purity.

Cocktail Antibodies:

Mouse anti C-V-OSCP [4C11C10D12] monoclonal:
Amount: 12.5 µg
Working concentration: 0.5 µg/ml

Mouse anti PDH-E1-alpha [9H9AF5] monoclonal:
Amount: 25 µg
Working concentration: 1 µg/ml

Mouse anti PDH-E1-beta [17A5E2H8] monoclonal:
Amount: 62.5 µg
Working concentration: 2.5 µg/ml

Mouse anti PDH-E2 [15D3G9C11] monoclonal:
Amount: 25 µg
Working concentration: 1 µg/ml

Mouse anti PDH-E2/E3bp [13G2AE2BH5] monoclonal:
Amount: 25 µg
Working concentration: 1 µg/ml

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

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Publications (35)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 82:103606 PubMed40158257

2025

Oncogene-induced senescence mitochondrial metabolism and bioenergetics drive the secretory phenotype: further characterization and comparison with other senescence-inducing stimuli.

Applications

Unspecified application

Species

Unspecified reactive species

Inés Marmisolle,Eliana Chacón,Santiago Mansilla,Santiago Ruiz,Mariana Bresque,Jennyfer Martínez,Ricardo Iván Martínez-Zamudio,Utz Herbig,Jie Liu,Toren Finkel,Carlos Escande,Laura Castro,Celia Quijano

Frontiers in cardiovascular medicine 11:1349417 PubMed38525191

2024

Reduced mitochondrial pyruvate carrier expression in hearts with heart failure and reduced ejection fraction patients: ischemic vs. non-ischemic origin.

Applications

Unspecified application

Species

Unspecified reactive species

Paula Lopez-Vazquez,Mariana Fernandez-Caggiano,Eduardo Barge-Caballero,Gonzalo Barge-Caballero,David Couto-Mallon,Zulaika Grille-Cancela,Paula Blanco-Canosa,Maria J Paniagua-Martin,Daniel Enriquez-Vazquez,Jose M Vazquez-Rodriguez,Nieves Domenech,Maria G Crespo-Leiro

Journal of physiology and pharmacology : an official journal of the Polish Physiological Society 73: PubMed37087564

2023

Assessment of mitochondrial respiratory capacity in relation to cardiac contractile performance in patients with normal to mildly decreased cardiac systolic function.

Applications

Unspecified application

Species

Unspecified reactive species

M Ljubkovic,F Runjic,C Bulat,M Cavar,L Frankovic,Z Marovic,N Bilopavlovic,J Marinovic

Biomedicines 10: PubMed35203575

2022

Identification of Novel Mitochondrial Pyruvate Carrier Inhibitors by Homology Modeling and Pharmacophore-Based Virtual Screening.

Applications

Unspecified application

Species

Unspecified reactive species

Lamees Hegazy,Lauren E Gill,Kelly D Pyles,Christopher Kaiho,Sophia Kchouk,Brian N Finck,Kyle S McCommis,Bahaa Elgendy

Cancers 13: PubMed34439291

2021

Non-Canonical Role of PDK1 as a Negative Regulator of Apoptosis through Macromolecular Complexes Assembly at the ER-Mitochondria Interface in Oncogene-Driven NSCLC.

Applications

Unspecified application

Species

Unspecified reactive species

Viviana De Rosa,Francesca Iommelli,Cristina Terlizzi,Eleonora Leggiero,Rosa Camerlingo,Giovanna G Altobelli,Rosa Fonti,Lucio Pastore,Silvana Del Vecchio

Journal of orthopaedic research : official publication of the Orthopaedic Research Society 40:1026-1038 PubMed34185335

2021

MuRF1 deficiency prevents age-related fat weight gain, possibly through accumulation of PDK4 in skeletal muscle mitochondria in older mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kosuke Sugiura,Katsuya Hirasaka,Tasuku Maeda,Takayuki Uchida,Koji Kishimoto,Motoko Oarada,Siegfried Labeit,Anayt Ulla,Iori Sakakibara,Reiko Nakao,Koichi Sairyo,Takeshi Nikawa

JCI insight 6: PubMed33986192

2021

Myocardial Lipin 1 knockout in mice approximates cardiac effects of human LPIN1 mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Kari T Chambers,Michael A Cooper,Alison R Swearingen,Rita T Brookheart,George G Schweitzer,Carla J Weinheimer,Attila Kovacs,Timothy R Koves,Deborah M Muoio,Kyle S McCommis,Brian N Finck

Redox biology 36:101660 PubMed32750667

2020

Pleiotropic effects of mdivi-1 in altering mitochondrial dynamics, respiration, and autophagy in cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Richa Aishwarya,Shafiul Alam,Chowdhury S Abdullah,Mahboob Morshed,Sadia S Nitu,Manikandan Panchatcharam,Sumitra Miriyala,Christopher G Kevil,Md Shenuarin Bhuiyan

Journal of clinical medicine 9: PubMed32276303

2020

Beneficial Effects of Acetyl-DL-Leucine (ADLL) in a Mouse Model of Sandhoff Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Ecem Kaya,David A Smith,Claire Smith,Barry Boland,Michael Strupp,Frances M Platt

Diabetes 68:1924-1933 PubMed31391173

2019

Disturbed Fatty Acid Oxidation, Endoplasmic Reticulum Stress, and Apoptosis in Left Ventricle of Patients With Type 2 Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Marko Ljubkovic,Melanie Gressette,Cristijan Bulat,Marija Cavar,Darija Bakovic,Damir Fabijanic,Ivica Grkovic,Christophe Lemaire,Jasna Marinovic

View all publications

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Product promise

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Pyruvate dehydrogenase (PDH) WB Antibody Cocktail

Western blot - Pyruvate dehydrogenase (PDH) WB Antibody Cocktail (AB110416)

Key facts

Applications

Reacts with

Form

Storage buffer

Reactivity data

Product details

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (35)

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