Total OXPHOS Human Western Blot Antibody Cocktail ab110411 is an optimized cocktail of antibodies for analyzing relative OXPHOS complex levels in Human mitochondria by Western Blot.
- Can detect multiple complexes at once, including complexes I, II, III and IV
- Cited in over 460 publications
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- Life (Basel, Switzerland) 15:2025Active Substances from the Micro-Immunotherapy Medicine 2LMIREG Display Antioxidative Properties In Vitro in Two Colorectal Cancer Cell Lines.Applications:WBReactive species:HumanLaura Garcia-Sureda,Camille Jacques,Daniel G Pons,Jorge Sastre-Serra,Jordi Oliver,Ilaria FlorisPubMed 40430171
- Pathology, research and practice 264:1557132024Polyribonucleotide phosphorylase is overexpressed in hepatocellular cancer, promoting epithelial phenotype maintenance and tumor progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrancisco Revert-Ros,Ignacio Ventura,Jesús A Prieto-Ruiz,Eduardo Giner-Moreno,Ángela Pérez-Cervera,Judith Pérez-Rojas,Fernando Revert,José Miguel Hernández-AndreuPubMed 39522317
- Biochemical pharmacology 220:1160102023Divergent effects of the antiretroviral drugs, dolutegravir, tenofovir alafenamide, and tenofovir disoproxil fumarate, on human adipocyte function.Applications:WBReactive species:HumanT Quesada-López,R Cereijo,A Blasco-Roset,A Mestres-Arenas,P Prieto,J C Domingo,F Villarroya,P Domingo,M GiraltPubMed 38154544
- Journal of translational medicine 21:8762023Tigecycline causes loss of cell viability mediated by mitochondrial OXPHOS and RAC1 in hepatocellular carcinoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDominik T Koch,Haochen Yu,Iris Beirith,Malte Schirren,Moritz Drefs,Yunfei Liu,Mathilda Knoblauch,Dionysios Koliogiannis,Weiwei Sheng,Enrico N De Toni,Alexandr V Bazhin,Bernhard W Renz,Markus O Guba,Jens Werner,Matthias IlmerPubMed 38041179
- Frontiers in genetics 14:12785722023Prominent muscle involvement in a familial form of mitochondrial disease due to a variant.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMartina Rimoldi,Francesca Magri,Sara Antognozzi,Michela Ripolone,Sabrina Salani,Daniela Piga,Letizia Bertolasi,Simona Zanotti,Patrizia Ciscato,Francesco Fortunato,Maurizio Moggio,Stefania Corti,Giacomo Pietro Comi,Dario RonchiPubMed 38098475
- Nutrients 15:2023Lactate Conversion by Lactate Dehydrogenase B Is Involved in Beige Adipocyte Differentiation and Thermogenesis in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBin Guo,Hui Shu,Ling Luo,Xiangpeng Liu,Yue Ma,Jie Zhang,Zhiwei Liu,Yong Zhang,Lei Fu,Tongxing Song,Yixue Qiao,Chi ZhangPubMed 38004240
- Cancer & metabolism 11:202023ATM inhibition blocks glucose metabolism and amplifies the sensitivity of resistant lung cancer cell lines to oncogene driver inhibitors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCristina Terlizzi,Viviana De Rosa,Francesca Iommelli,Antonio Pezone,Giovanna G Altobelli,Maurizio Maddalena,Jelena Dimitrov,Caterina De Rosa,Carminia Maria Della Corte,Vittorio Enrico Avvedimento,Silvana Del VecchioPubMed 37932830
- Current research in neurobiology 5:1001122023The SARS-CoV-2 spike glycoprotein interacts with MAO-B and impairs mitochondrial energetics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChantal A Pileggi,Gaganvir Parmar,Hussein Elkhatib,Corina M Stewart,Irina Alecu,Marceline Côté,Steffany A L Bennett,Jagdeep K Sandhu,Miroslava Cuperlovic-Culf,Mary-Ellen HarperPubMed 38020812
- eLife 12:2023ONC201/TIC10 enhances durability of mTOR inhibitor everolimus in metastatic ER+ breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElena Farmaki,Aritro Nath,Rena Emond,Kimya L Karimi,Vince K Grolmusz,Patrick A Cosgrove,Andrea H BildPubMed 37772709
- iScience 26:1075372023Ets1 facilitates EMT/invasion through Drp1-mediated mitochondrial fragmentation in ovarian cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDeepshikha Ghosh,Suman Pakhira,Damayanti Das Ghosh,Susanta Roychoudhury,Sib Sankar RoyPubMed 37664613
Key facts
- Applications
- WB
- Target
- NDUFB8
(See target data below) - Reactive species
- Human
- Form
- Liquid
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Reacts | Dilution info - | Notes Recommended starting dilutions is 1:200 - 1:1000. However, optimal diltuion should be established by the end-user. We recommend using a high pH CAPS / PVDF transfer protocol when using this antibody for Western blot. Please contact us for more details. We do not recommend to boiling samples since it will cause signal reduction of several bands. We recommend warming to 37°C for 30 minutes prior to loading. |
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Target data
Function
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Additional Targets
SDHB, UQCRC2, MT-CO1, ATP5F1A
Alternative names
Complex I-ASHI, NADH-ubiquinone oxidoreductase ASHI subunit, CI-ASHI, NDUFB8
Recommended products
Total OXPHOS Human Western Blot Antibody Cocktail ab110411 is an optimized cocktail of antibodies for analyzing relative OXPHOS complex levels in Human mitochondria by Western Blot.
- Can detect multiple complexes at once, including complexes I, II, III and IV
- Cited in over 460 publications
Key facts
- Applications
- WB
- Target
- NDUFB8
- Reactive species
- Human
- Form
- Liquid
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 0.15% HEPES
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab110411 contains 5 mAbs, one each against Complex I subunit NDUFB8 (Anti-NDUFB8 antibody [20E9DH10C12] ab110242), Complex II subunit 30kDa (Anti-SDHB antibody [21A11AE7] ab14714), Complex III subunit Core 2 (Anti-UQCRC2 antibody [13G12AF12BB11] ab14745), Complex IV subunit II (Anti-MTCO2 antibody [12C4F12] ab110258), and ATP synthase subunit alpha (Anti-ATP5A antibody [15H4C4] - Mitochondrial Marker ab14748) as an optimized premixed cocktail. The cocktail is suitable for Western Blotting analysis of the relative levels of the 5 OXPHOS complexes in mitochondrial preparations from human sources.
Altered levels of assembly can arise from mutations in individual subunits, mutations in assembly factors for the complex(es), mtDNA depletion or as a result of physiological and or pathological changes e.g. hormone treatment, exercise, diet or oxidative stress.
The mAbs in the cocktail were chosen because they are against a subunit that is labile when its complex is not assembled. Moreover, the combination is readily resolved in SDS-PAGE when the appropriate gel conditions are used (Please refer to application notes).
The Western blot cocktail is supplied at a concentration of 1.5 mg/mL.
The five antibodies provided in the cocktail are all mouse monoclonal antibodies.
We recommend using a high pH CAPS buffer / PVDF transfer protocol when using this antibody for Western blot. Please contact us for more details.
Due to a policy change, the previously provided control for this kit has been removed.
We do not recommend to boiling samples since it will cause signal reduction of several bands. We recommend warming to 37°C for 30 minutes prior to loading.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Western blot - Total OXPHOS Human WB Antibody Cocktail (ab110411)
Each lane contains 10 µg of mitochondria isolated from MRC5 fibroblasts.
ab110411(MS601) was used to label lane 6, and the individual antibodies
comprising the cocktail were used for lanes 1-5.
This image is courtesy of an anonymous customer reviewWestern blot - Total OXPHOS Human WB Antibody Cocktail (ab110411)
Gel: Non-reduced Denaturing (4-12%)
Lanes 1-3: contain 3 individual Human B cell lymphoma samples 50 μg loaded per lane.
Blocked for 1 hour with 5% Milk at room temperature.
ab110411 used as per instructions.
Secondary: HRP-conjugated anti-mouse IgG uesd at 1/300
Detection method: ECL+ 2 minutes exposure.
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