AB110411

Total OXPHOS Human WB Antibody Cocktail

Name: Total OXPHOS Human Western Blot Antibody Cocktail (ab110411) | Abcam
Brand: Abcam Limited
SKU: AB110411
Rating: 5 (4 reviews)

(4 Reviews)

(585 Publications)

Total OXPHOS Human Western Blot Antibody Cocktail ab110411 is an optimized cocktail of antibodies for analyzing relative OXPHOS complex levels in Human mitochondria by Western Blot.

- Can detect multiple complexes at once, including complexes I, II, III and IV
- Cited in over 460 publications

View Alternative Names

Complex I-ASHI, NADH-ubiquinone oxidoreductase ASHI subunit, CI-ASHI, NDUFB8

2 Images

Supplier Data

Western blot - Total OXPHOS Human WB Antibody Cocktail (AB110411)

Each lane contains 10 µg of mitochondria isolated from MRC5 fibroblasts.
ab110411(MS601) was used to label lane 6, and the individual antibodies
comprising the cocktail were used for lanes 1-5.

false

AbReview

Western blot - Total OXPHOS Human WB Antibody Cocktail (AB110411)

Gel : Non-reduced Denaturing (4-12%)

Lanes 1-3 : contain 3 individual Human B cell lymphoma samples 50 μg loaded per lane.

Blocked for 1 hour with 5% Milk at room temperature.

ab110411 used as per instructions.

Secondary : HRP-conjugated anti-mouse IgG uesd at 1/300

Detection method : ECL+ 2 minutes exposure.

false

This image is courtesy of an anonymous Abreview

Key facts

Applications

applications

Target

NDUFB8

target

Reacts with

Human

Form

Liquid

form

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 0.15% HEPES

storage-buffer

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Recommended starting dilutions is 1:200 - 1:1000. However, optimal diltuion should be established by the end-user.We recommend using a high pH CAPS / PVDF transfer protocol when using this antibody for Western blot. Please contact us for more details.We do not recommend to boiling samples since it will cause signal reduction of several bands. We recommend warming to 37°C for 30 minutes prior to loading." } } }

style

left-column

Product details

ab110411 contains 5 mAbs, one each against Complex I subunit NDUFB8 (ab110242), Complex II subunit 30kDa (ab14714), Complex III subunit Core 2 (ab14745), Complex IV subunit II (ab110258), and ATP synthase subunit alpha (ab14748) as an optimized premixed cocktail. The cocktail is suitable for Western Blotting analysis of the relative levels of the 5 OXPHOS complexes in mitochondrial preparations from human sources.

Altered levels of assembly can arise from mutations in individual subunits, mutations in assembly factors for the complex(es), mtDNA depletion or as a result of physiological and or pathological changes e.g. hormone treatment, exercise, diet or oxidative stress.

The mAbs in the cocktail were chosen because they are against a subunit that is labile when its complex is not assembled. Moreover, the combination is readily resolved in SDS-PAGE when the appropriate gel conditions are used (Please refer to application notes).

The Western blot cocktail is supplied at a concentration of 1.5 mg/mL.

The five antibodies provided in the cocktail are all mouse monoclonal antibodies.

We recommend using a high pH CAPS buffer / PVDF transfer protocol when using this antibody for Western blot. Please contact us for more details.

Due to a policy change, the previously provided control for this kit has been removed.

We do not recommend to boiling samples since it will cause signal reduction of several bands. We recommend warming to 37°C for 30 minutes prior to loading.

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

style

left-column

style

left-column

style

left-column

Product protocols

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

See full target information NDUFB8

Additional targets

SDHB,UQCRC2,MT-CO1,ATP5F1A

style

left-column

Publications (585)

Recent publications for all applications. Explore the full list and refine your search

Oxidative medicine and cellular longevity 2025:5589475 PubMed41031329

2025

Caffeine, MitoQ, and GABA Prophylaxis of Mitochondrial Dysfunction Induced in Human Pulmonary Cells by Normobaric-Hyperoxia and Hyperbaric-Hyperoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Tanvir Hossain,Jackson T Secor,David M Eckmann

The Journal of physiology 603:5529-5545 PubMed40946331

2025

Clenbuterol induces lean mass and muscle protein accretion, but attenuates cardiorespiratory fitness and desensitizes muscle β-adrenergic signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Morten Hostrup,Lukas Moesgaard,Mads Fischer,Kate Aiko Wickham,Mads Pleshardt,Andreas Breenfeldt Andersen,Jacob Bejder,Martin Thomassen,Jens J Nielsen,Yvette Dehnes,Jens Bangsbo,Nikolai B Nordsborg,Søren Jessen

Molecular metabolism 100:102237 PubMed40848971

2025

ACAA1 knockout increases the survival rate of KPC mice by activating autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Ho Lee,Mingyu Kang,Sung Hoon Sim,Joon Hee Kang,Wonyoung Choi,Jung Won Chun,Woosol Hong,Chaeyoung Kim,Woojin Ham,Jeong Hwan Park,Eun-Byeol Koh,Yoon Jeon,Sang Myung Woo,Soo-Youl Kim

Molecular psychiatry 30:5833-5848 PubMed40847005

2025

Differential effects of lithium on metabolic dysfunctions in astrocytes derived from bipolar disorder patients.

Applications

Unspecified application

Species

Unspecified reactive species

Gyu Hyeon Baek,Dayeon Kim,Geurim Son,Hyunsu Do,Gyu-Bum Yeon,Mahn Jae Lee,Moongi Ji,Ji-Hoon Son,Mingyu Ju,Insook Ahn,Chanhee S Kang,Haeun Lee,Sungwoo Choi,Jae Myoung Suh,Jinsoo Seo,Fred H Gage,Man-Jeong Paik,YongKeun Park,Dae-Sung Kim,Jinju Han

International journal of molecular sciences 26: PubMed40806782

2025

The Loss of Complex I in Renal Oncocytoma Is Associated with Defective Mitophagy Due to Lysosomal Dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Lin,Neal Patel,Lucia Fernandez-Del-Rio,Cristiane Benica,Blake Wilde,Eirini Christodoulou,Shinji Ohtake,Anhyo Jeong,Aboubacar Kaba,Nedas Matulionis,Randy Caliliw,Xiaowu Gai,Heather Christofk,David Shackelford,Brian Shuch

Scientific reports 15:29167 PubMed40783579

2025

Therapeutic potential of human mesenchymal stromal cell-derived mitochondria in a rat model of surgical digestive fistula.

Applications

Unspecified application

Species

Unspecified reactive species

Antoine Mariani,Augustin Guichard,Anna C Sebbagh,André Cronemberger Andrade,Zahra Al Amir Dache,Christopher Ribes,Dmitry Ayollo,Mehdi Karoui,Gregory Lavieu,Florence Gazeau,Amanda K A Silva,Gabriel Rahmi,Sabah Mozafari

Nature metabolism 7:1570-1592 PubMed40691304

2025

LONP1 regulation of mitochondrial protein folding provides insight into beta cell failure in type 2 diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Li,Yamei Deng,Marie Gasser,Jie Zhu,Emily M Walker,Vaibhav Sidarala,Emma C Reck,Dre L Hubers,Mabelle B Pasmooij,Chun-Shik Shin,Khushdeep Bandesh,Eftyhmios Motakis,Siddhi Nargund,Romy Kursawe,Venkatesha Basrur,Alexey I Nesvizhskii,Michael L Stitzel,David C Chan,Guy A Rutter,Scott A Soleimanpour

iScience 28:112819 PubMed40662198

2025

B cell-derived nociceptin/orphanin FQ contributes to impaired glucose tolerance and insulin resistance in obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Stephanie C Puente-Ruiz,Leona Ide,Julia Schuller,Adel Ben-Kraiem,Anne Hoffmann,Adhideb Ghosh,Falko Noé,Christian Wolfrum,Kerstin Krause,Martin Gericke,Nora Klöting,Jens C Brüning,F Thomas Wunderlich,Matthias Blüher,Alexander Jais

NPJ systems biology and applications 11:67 PubMed40593928

2025

Temporal analysis of doxorubicin-induced cardiac toxicity and hypertrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Te Lin,Yi-Ju Lee,Wen-Wei Tseng,Zih-Hua Chen,Huai-Ching Hsieh,Ko-Hong Lin,Jin-Yu Su,An-Chi Wei

Frontiers in cell and developmental biology 13:1572571 PubMed40530336

2025

Establishment of human periodontal ligament cell lines with mutations to mimic dental aspects of hypophosphatasia.

Applications

Unspecified application

Species

Unspecified reactive species

Jana Schiffmaier,Sofia Rehling,Katharina Marnet,Angela Borst,Drenka Trivanović,Denitsa Docheva,Franz Jakob,Stephanie Graser,Marietta Herrmann,Daniel Liedtke

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Total OXPHOS Human WB Antibody Cocktail

Western blot - Total OXPHOS Human WB Antibody Cocktail (AB110411)

Western blot - Total OXPHOS Human WB Antibody Cocktail (AB110411)

Key facts

Applications

Target

Reacts with

Form

Storage buffer

Reactivity data

Product details

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Additional targets

Publications (585)

Complementary Products

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise