Total OXPHOS Rodent WB Antibody Cocktail

• WB

Supplier Data

Western blot - Total OXPHOS Rodent WB Antibody Cocktail (AB110413)

Rat liver mitochondria labeled with ab110413 (MS604). The sample in lane 1 was kept at room temperature, whereas the remaining three samples were heated to 37°C, 50°C, and 100°C, respectively. This blot shows that boiling of samples leads to a decrease in signal due to aggregation of proteins therefore heating samples at or close to boiling is not recommended.

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• WB

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Western blot - Total OXPHOS Rodent WB Antibody Cocktail (AB110413)

Isolated mitochondria from heart of human (5 μg - Lane 1), cow (1 ug - Lane 2), rat (10 μg - Lane 3), mouse (10 μg - Lane 4), HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate (20 μg -Lane 5) labeling CII-30kDa with ab14714 at 5 μg/mL. Secondary antibody is a goat anti-mouse antibody.

Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.

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• WB

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Western blot - Total OXPHOS Rodent WB Antibody Cocktail (AB110413)

Isolated mitochondria from heart of human (5 μg - Lane 1), cow (1 ug - Lane 2), rat (10 μg - Lane 3), mouse (10 μg - Lane 4) labeling CI subunit NDUFB8 with ab110242 at 0.5 ug/ml.

Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.

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• WB

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Western blot - Total OXPHOS Rodent WB Antibody Cocktail (AB110413)

Isolated mitochondria from heart of human (5 μg - Lane 1), cow (1 ug - Lane 2), rat (10 μg - Lane 3), mouse (5 μg - Lane 4) labeling CIV subunit I with ab14705 at 0.5 μg/mL. Secondary antibody is a goat anti-mouse antibody.

Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.

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• WB

Unknown

Western blot - Total OXPHOS Rodent WB Antibody Cocktail (AB110413)

Isolated mitochondria from heart of human (10 μg - Lane 1), cow (4 μg - Lane 2), rat (10 μg - Lane 3), mouse (10 μg - Lane 4), HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate (20 μg -Lane 5) labeling CV alpha subunit with ab14748 at 1 μg/mL

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• WB

Unknown

Western blot - Total OXPHOS Rodent WB Antibody Cocktail (AB110413)

Human liver tissue lysate, (10 μg - Lane 1) and HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate (10 μg - Lane 2) labeling CV alpha subunit with ab14748 at 1 μg/mL. Secondary antibody is a goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP), 1/3000 dilution.

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• WB

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Western blot - Total OXPHOS Rodent WB Antibody Cocktail (AB110413)

Human skeletal muscle tissue lysate, (10 μg - Lane 1) and Ramos (human Burkitt's lymphoma cell line) whole cell lysate (10 μg - Lane 2) labeling CIII-Core protein 2 with ab14745 at 5 μg/mL. Secondary antibody is a goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP), 1/3000 dilution.

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• WB

AbReview

Western blot - Total OXPHOS Rodent WB Antibody Cocktail (AB110413)

Isolated mitochondria from mice brain (control and Alzheimer's disease (AD)) labeled with ab110413 at 1/1000 dilution in 5% BSA.

This image shows NADH dehydrogenase beta subcomplex subunit 8 of Complex I (NDUFB8), succinate dehydrogenase subunit B of Complex II (SDHB), cytochrome c oxidase subunit 1 of Complex IV (MTCO1), cytochrome b-c1 complex subunit 2 of Complex III (UQCRC2) and ATP synthase subunit alpha of Complex V (ATP5A).

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This image is courtesy of Aida Adlimoghaddam

• WB

CiteAb

Western blot - Total OXPHOS Rodent WB Antibody Cocktail (AB110413)

Western Blotting using Total OXPHOS Rodent WB Antibody Cocktail, ab110413. Publication image from Karch, J. et al., 2017, Elife, 29148970. Legend direct from paper.

Bax and Bak localize to lysosomes and targeting Bax to lysosomes restores autophagic cell death in DKO MEFs.(A) Western blot analysis of the indicated protein fractions from starved and control WT MEFs for Bax, Bak, Lamp1 and the oxidative phosphorylation mitochondrial proteins UQCRC2 (upper band) and MTCO1 (lower band). (B) Transmission EM image of DKO MEFs treated with an adenovirus for mini SOG-Bax. The yellow arrows show electron dense mini-SOG-Bax within the mitochondrial membranes while the pink arrow heads show electron dense regions within the autolysosomes and lysosomes. Scale bar = 1 μm. (C) DKO MEFs infected with a Dox-inducible adenovirus for Bax containing the lysosomal targeting sequence of Lamp1 on its C-terminus. The infected cells were then treated with or without Dox to induce expression and then subjected to serum starvation to induce autophagic cell death, or treated with 200 nM staurosporine to induce apoptotic cell death. Cell death was determined by plasma membrane rupture. All assays are an average or are representative of three independent experiments. The error bars represent the standard error of the mean. *p<0.05 vs no Dox with students t-test.10.7554/eLife.30543.010Figure 4—source data 1.Raw western gel images for Figure 4A.Raw western gel images for Figure 4A.

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• WB

CiteAb

Western blot - Total OXPHOS Rodent WB Antibody Cocktail (AB110413)

Western Blotting using Total OXPHOS Rodent WB Antibody Cocktail, ab110413. Publication image from Phielix, E. et al., 2017, Nutr Metab (Lond), 28616059. Legend direct from paper.

Effect of postnatal Concept diet on relative protein expression of 5 oxidative phosphorylation complex (OXPHOS) subunits (a – e) in the retroperitoneal (RP) white adipose tissue (n = 8 for CTRL, n = 7 for Concept and n = 10 for REF group) and (f) total protein and western blot bands of one sample per group, samples came from one blot, but not from adjacent lanes, a picture of the whole blot is added as Additional file 1. Early diet and WSD challenge effects were analyzed separately. Difference between Concept and REF group not tested, as groups were fed different postnatal and adult diets. * : p < 0.05, Concept different from CTRL group; † : p 0.05–0.1 REF different from CTRL group.

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