Rabbit Recombinant Monoclonal TAU antibody. Suitable for WB, IHC-P, IHC-Fr and reacts with Synthetic peptide, Human, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
WB | IHC-P | IHC-Fr | |
---|---|---|---|
Human | Tested | Tested | Expected |
Rat | Expected | Expected | Tested |
Synthetic peptide | Tested | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info 1/500 | Notes - |
Species Human | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info 1/500 | Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
Species Rat | Dilution info 1/500 | Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
MAPT
MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
Rabbit Recombinant Monoclonal TAU antibody. Suitable for WB, IHC-P, IHC-Fr and reacts with Synthetic peptide, Human, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR21726
Affinity purification Protein A
The specificity of this antibody refers to P10636-2.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
The 0N Tau protein also known by its alternate names MAPT (microtubule-associated protein tau) and non-N-terminal tau serves important mechanical functions in the stabilization of microtubules within neurons. It does so by binding to and promoting microtubule assembly and maintenance. The mass of the 0N Tau isoform varies because of alternative splicing but it is a critical component of neurofibrillary structures. This protein expression occurs primarily in neurons but can also be found in glial cells of the central nervous system.
The stabilization function of the 0N Tau isoform influences the cellular cytoskeleton's structural integrity which is necessary for accurate neuronal transport and function. It participates in the microtubule complex which forms the network necessary for intracellular transport and structural support. Stability and dynamics of this network allow efficient transport of nutrients organelles and signaling molecules within the cells playing an important role in maintaining neuronal health and function.
The 0N Tau protein is involved in critical pathways like the MAPK and PI3K-Akt signaling pathways. These pathways influence cell survival proliferation and apoptotic processes. Proteins like glycogen synthase kinase-3 beta (GSK-3β) regulate phosphorylation of tau affecting its binding to microtubules and subsequent cellular dynamics. Additionally hyperphosphorylation of tau by kinases such as CDK5 modifies its function and alters cellular pathways.
The role of 0N Tau becomes significant in neurodegenerative conditions like Alzheimer's disease and frontotemporal dementia. These disorders are associated with aberrant phosphorylation and aggregation of tau resulting in pathological tau tangles. The relationship of 0N Tau with amyloid-beta peptides also links it to Alzheimer's disease further highlighting its involvement in neurodegeneration. Proteins like amyloid precursor protein (APP) connect with tau pathology within these diseases contributing to neuronal dysfunction and degeneration.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling 0N Tau with ab218199 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on human cerebrum (PMID: 17183532) is observed. Counterstained with hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-0N Tau antibody [EPR21726] (ab218199) at 1/1000 dilution
Lane 1: Human hippocampus lysate at 20 µg
Lane 2: Human brain lysate at 20 µg
Lane 3: Human stomach lysate at 20 µg
All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Predicted band size: 79 kDa
Exposure time: 3min
This antibody specifically recognizes 0N3R and 0N4R tau recombinant proteins. The lower weak bands maybe degraded tau fragments (PMID:28045602).
All lanes: Western blot - Anti-0N Tau antibody [EPR21726] (ab218199) at 1/1000 dilution
Lane 1: His-tagged human 2N3R Tau recombinant protein (aa1-410) 10 ng
Lane 2: His-tagged human 2N4R Tau recombinant protein (aa1-441) 10ng
Lane 3: His-tagged human 0N3R Tau recombinant protein (aa1-352) 10ng
Lane 4: His-tagged human 0N4R Tau recombinant protein (aa1-383) 10ng
Lane 5: His-tagged human 1N3R Tau recombinant protein (aa1-381) 10ng
Lane 6: His-tagged human 1N4R Tau recombinant protein (aa1-412) 10ng
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/1000 dilution
Predicted band size: 79 kDa
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat hippocampus tissue labeling 0N Tau with ab218199 at 1/500 dilution (green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1,000 dilution. Cytoplasmic staining on MF (mossy fibers) of rat hippocampus (PMID:18925637, PMID:24386422) is observed. Counterstained with DAPI (blue).Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1,000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).
Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling 0N Tau with ab218199 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on human breast carcinoma (PMID: 15914550) is observed. Counterstained with hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
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