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Rabbit Recombinant Monoclonal TAU antibody. Suitable for WB, IHC-P, IHC-Fr and reacts with Synthetic peptide, Human, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-0N Tau antibody [EPR21726] (AB218199), expandable thumbnail
  • Western blot - Anti-0N Tau antibody [EPR21726] (AB218199), expandable thumbnail
  • Western blot - Anti-0N Tau antibody [EPR21726] (AB218199), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-0N Tau antibody [EPR21726] (AB218199), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-0N Tau antibody [EPR21726] (AB218199), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PIHC-Fr
Human
Tested
Tested
Expected
Rat
Expected
Expected
Tested
Synthetic peptide
Tested
Tested
Tested

Tested
Tested

Species

Synthetic peptide

Dilution info
1/1000
Notes

-

Species

Human

Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Synthetic peptide

Dilution info
1/500
Notes

-

Species

Human

Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Synthetic peptide

Dilution info
1/500
Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Species

Rat

Dilution info
1/500
Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Targets

MAPT

Alternative names

Recommended products

Rabbit Recombinant Monoclonal TAU antibody. Suitable for WB, IHC-P, IHC-Fr and reacts with Synthetic peptide, Human, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR21726

Purification technique

Affinity purification Protein A

Specificity

The specificity of this antibody refers to P10636-2.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The 0N Tau protein also known by its alternate names MAPT (microtubule-associated protein tau) and non-N-terminal tau serves important mechanical functions in the stabilization of microtubules within neurons. It does so by binding to and promoting microtubule assembly and maintenance. The mass of the 0N Tau isoform varies because of alternative splicing but it is a critical component of neurofibrillary structures. This protein expression occurs primarily in neurons but can also be found in glial cells of the central nervous system.

Biological function summary

The stabilization function of the 0N Tau isoform influences the cellular cytoskeleton's structural integrity which is necessary for accurate neuronal transport and function. It participates in the microtubule complex which forms the network necessary for intracellular transport and structural support. Stability and dynamics of this network allow efficient transport of nutrients organelles and signaling molecules within the cells playing an important role in maintaining neuronal health and function.

Pathways

The 0N Tau protein is involved in critical pathways like the MAPK and PI3K-Akt signaling pathways. These pathways influence cell survival proliferation and apoptotic processes. Proteins like glycogen synthase kinase-3 beta (GSK-3β) regulate phosphorylation of tau affecting its binding to microtubules and subsequent cellular dynamics. Additionally hyperphosphorylation of tau by kinases such as CDK5 modifies its function and alters cellular pathways.

Associated diseases and disorders

The role of 0N Tau becomes significant in neurodegenerative conditions like Alzheimer's disease and frontotemporal dementia. These disorders are associated with aberrant phosphorylation and aggregation of tau resulting in pathological tau tangles. The relationship of 0N Tau with amyloid-beta peptides also links it to Alzheimer's disease further highlighting its involvement in neurodegeneration. Proteins like amyloid precursor protein (APP) connect with tau pathology within these diseases contributing to neuronal dysfunction and degeneration.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-0N Tau antibody [EPR21726] (ab218199), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-0N Tau antibody [EPR21726] (ab218199)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling 0N Tau with ab218199 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on human cerebrum (PMID: 17183532) is observed. Counterstained with hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-0N Tau antibody [EPR21726] (ab218199), expandable thumbnail

    Western blot - Anti-0N Tau antibody [EPR21726] (ab218199)

    Negative control: Human stomach PMID:8752131; PMID:11727254.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-0N Tau antibody [EPR21726] (ab218199) at 1/1000 dilution

    Lane 1: Human hippocampus lysate at 20 µg

    Lane 2: Human brain lysate at 20 µg

    Lane 3: Human stomach lysate at 20 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 79 kDa

    Exposure time: 3min

  • Western blot - Anti-0N Tau antibody [EPR21726] (ab218199), expandable thumbnail

    Western blot - Anti-0N Tau antibody [EPR21726] (ab218199)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    This antibody specifically recognizes 0N3R and 0N4R tau recombinant proteins. The lower weak bands maybe degraded tau fragments (PMID:28045602).

    All lanes: Western blot - Anti-0N Tau antibody [EPR21726] (ab218199) at 1/1000 dilution

    Lane 1: His-tagged human 2N3R Tau recombinant protein (aa1-410) 10 ng

    Lane 2: His-tagged human 2N4R Tau recombinant protein (aa1-441) 10ng

    Lane 3: His-tagged human 0N3R Tau recombinant protein (aa1-352) 10ng

    Lane 4: His-tagged human 0N4R Tau recombinant protein (aa1-383) 10ng

    Lane 5: His-tagged human 1N3R Tau recombinant protein (aa1-381) 10ng

    Lane 6: His-tagged human 1N4R Tau recombinant protein (aa1-412) 10ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/1000 dilution

    Predicted band size: 79 kDa

  • Immunohistochemistry (Frozen sections) - Anti-0N Tau antibody [EPR21726] (ab218199), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-0N Tau antibody [EPR21726] (ab218199)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat hippocampus tissue labeling 0N Tau with ab218199 at 1/500 dilution (green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1,000 dilution. Cytoplasmic staining on MF (mossy fibers) of rat hippocampus (PMID:18925637, PMID:24386422) is observed. Counterstained with DAPI (blue).Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1,000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).

  • Immunohistochemistry (Frozen sections) - Anti-0N Tau antibody [EPR21726] (ab218199), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-0N Tau antibody [EPR21726] (ab218199)

    Negative control - Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat stomach tissue labeling 0N Tau with ab218199 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1,000 dilution. No staining on rat stomach (PMID:11727254; PMID: 8752131) is observed. Counterstained with DAPI (blue).Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1,000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-0N Tau antibody [EPR21726] (ab218199), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-0N Tau antibody [EPR21726] (ab218199)

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling 0N Tau with ab218199 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on human breast carcinoma (PMID: 15914550) is observed. Counterstained with hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-0N Tau antibody [EPR21726] (ab218199), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-0N Tau antibody [EPR21726] (ab218199)

    Negative control - Immunohistochemical analysis of paraffin-embedded human liver tissue labeling 0N Tau with ab218199 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). No staining on human liver (PMID: 8752131) is observed. Counterstained with hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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