anti-0N Tau antibody [EPR21726] ab242344 is a carrier free rabbit monoclonal antibody that is used in 0N Tau western blotting and IHC. Suitable for human and rat samples.
- PBS only, conjugation-ready, removing anything extra from your antibodies, greater flexibility in assay design
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-0N Tau antibody [EPR21726] - BSA and Azide free (AB242344), expandable thumbnail](https://content.abcam.com/products/images/0n-tau-antibody-epr21726-bsa-and-azide-free-ab242344--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129192.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-0N Tau antibody [EPR21726] - BSA and Azide free (AB242344)")
![Immunohistochemistry (Frozen sections) - Anti-0N Tau antibody [EPR21726] - BSA and Azide free (AB242344), expandable thumbnail](https://content.abcam.com/products/images/0n-tau-antibody-epr21726-bsa-and-azide-free-ab242344--immunohistochemistry-frozen-sections-img121950.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-0N Tau antibody [EPR21726] - BSA and Azide free (AB242344)")
![Immunohistochemistry (Frozen sections) - Anti-0N Tau antibody [EPR21726] - BSA and Azide free (AB242344), expandable thumbnail](https://content.abcam.com/products/images/0n-tau-antibody-epr21726-bsa-and-azide-free-ab242344--immunohistochemistry-frozen-sections-img134474.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-0N Tau antibody [EPR21726] - BSA and Azide free (AB242344)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-0N Tau antibody [EPR21726] - BSA and Azide free (AB242344), expandable thumbnail](https://content.abcam.com/products/images/0n-tau-antibody-epr21726-bsa-and-azide-free-ab242344--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135966.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-0N Tau antibody [EPR21726] - BSA and Azide free (AB242344)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-0N Tau antibody [EPR21726] - BSA and Azide free (AB242344), expandable thumbnail](https://content.abcam.com/products/images/0n-tau-antibody-epr21726-bsa-and-azide-free-ab242344--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107468.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-0N Tau antibody [EPR21726] - BSA and Azide free (AB242344)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-Fr | IHC-P | WB | |
|---|---|---|---|
| Human | Expected | Tested | Tested |
| Rat | Tested | Expected | Expected |
| Recombinant full length protein - Human | Not recommended | Not recommended | Tested |
| Synthetic peptide | Tested | Tested | Not recommended |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Synthetic peptide | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human, Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Target data
Function
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Additional Targets
MAPT
Alternative names
MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
Recommended products
anti-0N Tau antibody [EPR21726] ab242344 is a carrier free rabbit monoclonal antibody that is used in 0N Tau western blotting and IHC. Suitable for human and rat samples.
- PBS only, conjugation-ready, removing anything extra from your antibodies, greater flexibility in assay design
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR21726
- Purification technique
- Affinity purification Protein A
- Specificity
The specificity of this antibody refers to P10636-2.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab242344 is the carrier-free version of Anti-0N Tau antibody [EPR21726] ab218199.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The 0N Tau protein also known by its alternate names MAPT (microtubule-associated protein tau) and non-N-terminal tau serves important mechanical functions in the stabilization of microtubules within neurons. It does so by binding to and promoting microtubule assembly and maintenance. The mass of the 0N Tau isoform varies because of alternative splicing but it is a critical component of neurofibrillary structures. This protein expression occurs primarily in neurons but can also be found in glial cells of the central nervous system.
- Biological function summary
The stabilization function of the 0N Tau isoform influences the cellular cytoskeleton's structural integrity which is necessary for accurate neuronal transport and function. It participates in the microtubule complex which forms the network necessary for intracellular transport and structural support. Stability and dynamics of this network allow efficient transport of nutrients organelles and signaling molecules within the cells playing an important role in maintaining neuronal health and function.
- Pathways
The 0N Tau protein is involved in critical pathways like the MAPK and PI3K-Akt signaling pathways. These pathways influence cell survival proliferation and apoptotic processes. Proteins like glycogen synthase kinase-3 beta (GSK-3β) regulate phosphorylation of tau affecting its binding to microtubules and subsequent cellular dynamics. Additionally hyperphosphorylation of tau by kinases such as CDK5 modifies its function and alters cellular pathways.
- Associated diseases and disorders
The role of 0N Tau becomes significant in neurodegenerative conditions like Alzheimer's disease and frontotemporal dementia. These disorders are associated with aberrant phosphorylation and aggregation of tau resulting in pathological tau tangles. The relationship of 0N Tau with amyloid-beta peptides also links it to Alzheimer's disease further highlighting its involvement in neurodegeneration. Proteins like amyloid precursor protein (APP) connect with tau pathology within these diseases contributing to neuronal dysfunction and degeneration.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-0N Tau antibody [EPR21726] - BSA and Azide free (ab242344), expandable thumbnail](https://content.abcam.com/products/images/0n-tau-antibody-epr21726-bsa-and-azide-free-ab242344--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129192.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-0N Tau antibody [EPR21726] - BSA and Azide free (ab242344)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-0N Tau antibody [EPR21726] - BSA and Azide free (ab242344)
Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Tau with Anti-0N Tau antibody [EPR21726] ab218199 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on human cerebrum (PMID: 17183532) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.
![Immunohistochemistry (Frozen sections) - Anti-0N Tau antibody [EPR21726] - BSA and Azide free (ab242344), expandable thumbnail](https://content.abcam.com/products/images/0n-tau-antibody-epr21726-bsa-and-azide-free-ab242344--immunohistochemistry-frozen-sections-img121950.jpg "Immunohistochemistry (Frozen sections) - Anti-0N Tau antibody [EPR21726] - BSA and Azide free (ab242344)")
Immunohistochemistry (Frozen sections) - Anti-0N Tau antibody [EPR21726] - BSA and Azide free (ab242344)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat hippocampus tissue labeling Tau with Anti-0N Tau antibody [EPR21726] ab218199 at 1/500 dilution (green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1,000 dilution. Cytoplasmic staining on MF (mossy fibers) of rat hippocampus (PMID:18925637, PMID:24386422) is observed. Counterstained with DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1,000 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.
![Immunohistochemistry (Frozen sections) - Anti-0N Tau antibody [EPR21726] - BSA and Azide free (ab242344), expandable thumbnail](https://content.abcam.com/products/images/0n-tau-antibody-epr21726-bsa-and-azide-free-ab242344--immunohistochemistry-frozen-sections-img134474.jpg "Immunohistochemistry (Frozen sections) - Anti-0N Tau antibody [EPR21726] - BSA and Azide free (ab242344)")
Immunohistochemistry (Frozen sections) - Anti-0N Tau antibody [EPR21726] - BSA and Azide free (ab242344)
Negative control - Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat stomach tissue labeling Tau with Anti-0N Tau antibody [EPR21726] ab218199 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1,000 dilution. No staining on rat stomach (PMID:11727254; PMID: 8752131) is observed. Counterstained with DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1,000 dilution.Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-0N Tau antibody [EPR21726] - BSA and Azide free (ab242344), expandable thumbnail](https://content.abcam.com/products/images/0n-tau-antibody-epr21726-bsa-and-azide-free-ab242344--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135966.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-0N Tau antibody [EPR21726] - BSA and Azide free (ab242344)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-0N Tau antibody [EPR21726] - BSA and Azide free (ab242344)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling Tau with Anti-0N Tau antibody [EPR21726] ab218199 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on human breast carcinoma (PMID: 15914550) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-0N Tau antibody [EPR21726] - BSA and Azide free (ab242344), expandable thumbnail](https://content.abcam.com/products/images/0n-tau-antibody-epr21726-bsa-and-azide-free-ab242344--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107468.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-0N Tau antibody [EPR21726] - BSA and Azide free (ab242344)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-0N Tau antibody [EPR21726] - BSA and Azide free (ab242344)
Negative control - Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Tau with Anti-0N Tau antibody [EPR21726] ab218199 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). No staining on human liver (PMID: 8752131) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.
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