AB208199

Anti-1-methylguanosine (m1G) antibody [EPR19833-150]

20ul selling size
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal 1-methylguanosine (m1G) antibody. Suitable for IP, ELISA, FRET, Dot and reacts with Chemical, Synthetic peptide samples.

5 Images

Supplier Data

Dot Blot - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] (AB208199)

Dot blot analysis of 1-methylguanosine (m1G) using ab208199 at 1/500 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20,000 dilution (Left) or Methylene Blue (Right).

Lane 1 : HeLa total RNA

Lane 2 : Yeast tRNA

Exposure time : 8 seconds

ELISA - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] (AB208199)

ELISA

Supplier Data

ELISA - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] (AB208199)

BSA-conjugated m1G (modified) and G (unmodified) nucleosides were coated onto wells of a 96 well plate. ELISA was performed on 1.0 μg/ml of antigen using ab208199 at a concentration range of 0.005-4.000 μg/ml, followed by Goat Anti-Rabbit IgG, (H+L), alkaline phosphatase conjugated secondary antibody at 1/2500 dilution.

Supplier Data

Immunoprecipitation - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] (AB208199)

The IP was performed in a U-bottom non-adsorbing propylene 96-well plate.

ab208199 (0.2 μg) was coated into Dynabeads^® sheep-anti-rabbit IgG (50 μl) for 1h at RT.

Unmodified/modified oligonucleotides (5 μM) were added to samples containing the antibody/bead complexes and incubated with agitation for 1 hour at RT.

After washing, Peroxidase-conjugated Streptavidin was incubated at 1/1000 dilution with agitation for 1 hour at RT.

ECL substrate was then added and the results read in a non-transparent 96-well plate with a digital detector and analyzed using ImageJ.

Lane 1 : Buffer only.

Lane 2 : Modified oligonucleotide (5 μM), 5' Biotin-mN.mN.mN.mN.mN.[m1G]*.mN.mN.mN.mN.mN 3'

Lane 3 : Unmodified oligonucleotide (5 μM), 5' Biotin-mN.mN.mN.mN.mN.[G]*.mN.mN.mN.mN.mN 3'

N - equimolar mixture of (A/U/G/C)
m - 2'O methyl protection
* - phosphorothioate protection

Blocking buffer and concentration : 5% NFDM/TBST

Dilution buffer and concentration : TBST/0.1% Triton X-100/1 mM EDTA

All lanes:

Immunoprecipitation - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] (ab208199)

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FRET - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] (AB208199)

FRET

Supplier Data

FRET - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] (AB208199)

A FRET-based assay was performed using 25 nm of each biotinylated oligo using ab208199 at a concentration range of 0.001-4.000 µg/ml.

Supplier Data

Dot Blot - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] (AB208199)

Lane 1 : Biotin-m1G RNA oligo

Lane 2 : Biotin-G RNA oligo

Lane 3 : Biotin-m2G RNA oligo

Lane 4 : Biotin-m22G RNA oligo

Exposure time 5 seconds

Carrier free

Anti-1-methylguanosine (m1G) antibody [EPR19833-150] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19833-150

Isotype

IgG

Carrier free

Applications

Dot, FRET, ELISA, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "FRET" : {"fullname" : "FRET", "shortname":"FRET"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "0.005-4 µg/mL", "ELISA-species-notes": "", "FRET-species-checked": "testedAndGuaranteed", "FRET-species-dilution-info": "0.001-4 µg/mL", "FRET-species-notes": "HTRF readout", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "FRET-species-checked": "notRecommended", "FRET-species-dilution-info": "", "FRET-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/500", "Dot-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

1-methylguanosine (m1G) is a modified nucleoside with a distinct function in RNA biology. It is a methylated derivative of guanosine. This target plays an important role in RNA modification and stability. m1G results from the methylation of guanosine at the first position and has a molecular mass of about 167.15 g/mol. Expression of m1G occurs in various types of RNA across eukaryotic and prokaryotic organisms showing its widespread significance in biological systems. Although m1G itself is not a protein its presence impacts the mechanical aspects of the RNA molecule where it resides.

Biological function summary

M1G impacts RNA functionality by influencing RNA folding and structural conformation. It forms integral parts of RNA molecules and contributes to their stability and function. m1G does not act in isolation but is part of a broader set of modified nucleosides that collectively enhance RNA function. The modifications involving m1G occur as part of post-transcriptional modification processes. These are often important for ensuring proper decoding and function of RNA during gene expression.

Pathways

Research indicates that the presence of m1G within RNA molecules can influence specific biological pathways related to translation and RNA stability. m1G impacts the efficiency and fidelity of translational machinery playing a part in the translation pathway. Additionally it interacts with various proteins like tRNA methyltransferases which facilitate its methylation in RNA. These enzymes are critical in pathways that ensure the correctness of RNA modifications which ultimately influence protein synthesis.

Researchers associate aberrant levels of m1G with certain conditions such as neurodegenerative diseases and cancer. Disruption in the normal modification process of guanosine to m1G can lead to dysfunctional RNA which relates to disease pathology. Disorders in m1G modification often implicate other proteins involved in RNA modification like small nucleolar RNAs (snoRNAs) which assist in inserting this modification. Understanding m1G's role in disease progression helps develop targeted therapeutic strategies focusing on the modulation of these RNA-modifying enzymes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information 1-methylguanosine (m1G)

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-1-methylguanosine (m1G) antibody [EPR19833-150]

Dot Blot - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] (AB208199)

ELISA - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] (AB208199)

Immunoprecipitation - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] (AB208199)

FRET - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] (AB208199)

Dot Blot - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] (AB208199)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise