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Mouse Polyclonal 12 Lipoxygenase/ALOX12 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Polyunsaturated fatty acid lipoxygenase ALOX12.

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Images

Western blot - Anti-12 Lipoxygenase/ALOX12 antibody (AB167372), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-12 Lipoxygenase/ALOX12 antibody (AB167372), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Mouse

Storage buffer

pH: 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human Polyunsaturated fatty acid lipoxygenase ALOX12. Database link P18054

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1 µg/mL

Notes

-

Tested
Tested

Species

Human

Dilution info

10 µg/mL

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Catalyzes the regio and stereo-specific incorporation of molecular oxygen into free and esterified polyunsaturated fatty acids generating lipid hydroperoxides that can be further reduced to the corresponding hydroxy species (PubMed:17493578, PubMed:18311922, PubMed:1851637, PubMed:32404334, PubMed:8319693, PubMed:8500694). Mainly converts arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) to the specific bioactive lipid (12S)-hydroperoxyeicosatetraenoate/(12S)-HPETE (PubMed:17493578, PubMed:22984144, PubMed:24282679, PubMed:8319693, PubMed:8500694). Through the production of bioactive lipids like (12S)-HPETE it regulates different biological processes including platelet activation (PubMed:8319693, PubMed:8500694). It can also catalyze the epoxidation of double bonds of polyunsaturated fatty acids such as (14S)-hydroperoxy-docosahexaenoate/(14S)-HPDHA resulting in the formation of (13S,14S)-epoxy-DHA (PubMed:23504711). Furthermore, it may participate in the sequential oxidations of DHA ((4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate) to generate specialized pro-resolving mediators (SPMs) like resolvin D5 ((7S,17S)-diHPDHA) and (7S,14S)-diHPDHA, that actively down-regulate the immune response and have anti-aggregation properties with platelets (PubMed:32404334). An additional function involves a multistep process by which it transforms leukotriene A4/LTA4 into the bioactive lipids lipoxin A4/LXA4 and lipoxin B4/LXB4, both are vasoactive and LXA4 may regulate neutrophil function via occupancy of specific recognition sites (PubMed:8250832). Can also peroxidize linoleate ((9Z,12Z)-octadecadienoate) to (13S)-hydroperoxyoctadecadienoate/ (13S-HPODE) (By similarity). Due to its role in regulating both the expression of the vascular endothelial growth factor (VEGF, an angiogenic factor involved in the survival and metastasis of solid tumors) and the expression of integrin beta-1 (known to affect tumor cell migration and proliferation), it can be regarded as protumorigenic (PubMed:16638750, PubMed:22237009, PubMed:9751607). Important for cell survival, as it may play a role not only in proliferation but also in the prevention of apoptosis in vascular smooth muscle cells (PubMed:23578768).

Alternative names

12LO, LOG12, ALOX12, 12LO, LOG12, Polyunsaturated fatty acid lipoxygenase ALOX12, Arachidonate (12S)-lipoxygenase, Arachidonate (15S)-lipoxygenase, Linoleate (13S)-lipoxygenase, Lipoxin synthase 12-LO, Platelet-type lipoxygenase 12, 12S-LOX, 12S-lipoxygenase

Recommended products

Mouse Polyclonal 12 Lipoxygenase/ALOX12 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Polyunsaturated fatty acid lipoxygenase ALOX12.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Human Polyunsaturated fatty acid lipoxygenase ALOX12. Database link P18054
Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

12-Lipoxygenase (12-LOX) also known as ALOX12 is an enzyme that catalyzes the oxygenation of arachidonic acid to produce 12-hydroxyeicosatetraenoic acid (12-HETE). It has a molecular mass of approximately 75 kDa. This enzyme is widely expressed in various tissues including platelets lungs and pancreatic tissue. By converting polyunsaturated fatty acids such as arachidonic acid into bioactive lipids 12-LOX plays an important role in regulating cellular and physiological functions.

Biological function summary

12-LOX influences many cellular processes such as inflammation and thrombosis regulation. It is part of the lipoxygenase pathway synthesizing lipid signaling molecules that modulate immune responses and cellular growth. The enzyme does not form part of a large protein complex but interacts with lipid membranes where it exerts its catalytic function. Through its activity 12-LOX contributes to the production of eicosanoids which are signaling molecules with various physiological roles.

Pathways

12-LOX is involved in the arachidonic acid metabolism pathway and the eicosanoid signaling pathway. These pathways play roles in inflammation and immune response modulation. It works alongside other related lipoxygenases such as ALOX5 which produce different but related signaling molecules. The interaction between these enzymes helps maintain a balanced production of inflammatory mediators which controls cell proliferation and responses to external stimuli.

Associated diseases and disorders

Alterations in 12-LOX expression or function associate strongly with cardiovascular diseases and various types of cancers. In cardiovascular disease 12-LOX-derived eicosanoids contribute to the development of atherosclerosis by promoting inflammation and endothelial dysfunction. In cancer the dysregulation of 12-LOX can lead to increased tumor growth and metastasis. These conditions often exhibit altered interactions with proteins such as COX-2 another enzyme involved in eicosanoid production highlighting the role of 12-LOX in complex disease mechanisms.

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2 product images

  • Western blot - Anti-12 Lipoxygenase/ALOX12 antibody (ab167372), expandable thumbnail

    Western blot - Anti-12 Lipoxygenase/ALOX12 antibody (ab167372)

    All lanes: Western blot - Anti-12 Lipoxygenase/ALOX12 antibody (ab167372) at 1 µg/mL

    Lane 1: 12 Lipoxygenase/ALOX12 transfected 293T cell lysate at 15 µL

    Lane 2: Non-transfected 293T cell lysate at 15 µL

    Secondary

    All lanes: Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 76 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-12 Lipoxygenase/ALOX12 antibody (ab167372), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-12 Lipoxygenase/ALOX12 antibody (ab167372)

    Immunofluorescence analysis of HeLa cells labeling 12 Lipoxygenase/ALOX12 with ab167372 at 10μg/ml

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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