Rabbit Polyclonal 12 Lipoxygenase/ALOX12 antibody. C-terminal. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human ALOX12 aa 600-650 conjugated to Keyhole Limpet Haemocyanin.
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- Digital health 9:205520762312039022023Significance of platelet adhesion-related genes in colon cancer based on non-negative matrix factorization-based clustering algorithm.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao-Jv Chi,Yi-Bei Song,Deng-He Liu,Li-Qiang Wei,Xin An,Zi-Zhen Feng,Xiao-Hua Lan,Dong Lan,Chao HuangPubMed 37766908
- Cancer cell international 22:212022Development and validation of a novel 3-gene prognostic model for pancreatic adenocarcinoma based on ferroptosis-related genes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJihua Yang,XiaoHong Wei,Fang Hu,Wei Dong,Liao SunPubMed 35033072
- International journal of molecular sciences 22:2021MiR-7-5p Is Involved in Ferroptosis Signaling and Radioresistance Thru the Generation of ROS in Radioresistant HeLa and SAS Cell Lines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKazuo Tomita,Taisuke Nagasawa,Yoshikazu Kuwahara,Seiji Torii,Kento Igarashi,Mehryar Habibi Roudkenar,Amaneh Mohammadi Roushandeh,Akihiro Kurimasa,Tomoaki SatoPubMed 34361070
- iScience 24:1028372021Newcastle-disease-virus-induced ferroptosis through nutrient deprivation and ferritinophagy in tumor cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXianjin Kan,Yuncong Yin,Cuiping Song,Lei Tan,Xusheng Qiu,Ying Liao,Weiwei Liu,Songshu Meng,Yingjie Sun,Chan DingPubMed 34368653
- Biochimica et biophysica acta. Molecular and cell 1865:1588042020Pharmacological characterization of the biosynthesis of prostanoids and hydroxyeicosatetraenoic acids in human whole blood and platelets by targeted chiral lipidomics analysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStefania Tacconelli et. al.PubMed 32853794
- The journal of sexual medicine 17:1434-14472020Baicalein Alleviates Erectile Dysfunction Associated With Streptozotocin-Induced Type I Diabetes by Ameliorating Endothelial Nitric Oxide Synthase Dysfunction, Inhibiting Oxidative Stress and Fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYinwei Chen et. al.PubMed 32586748
- The American journal of pathology 189:2258-22682019Proresolving Mediators LXB4 and RvE1 Regulate Inflammation in Stromal Cells from Patients with Shoulder Tendon Tears.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStephanie G Dakin et. al.PubMed 31437425
- Cancer science 110:2856-28662019Lipid peroxidation increases hydrogen peroxide permeability leading to cell death in cancer cell lines that lack mtDNA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKazuo Tomita et. al.PubMed 31314163
- FASEB journal : official publication of the Federa 33:8043-8054201915-Epi-LXA and MaR1 counter inflammation in stromal cells from patients with Achilles tendinopathy and rupture.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStephanie G Dakin et. al.PubMed 30916999
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ALOX12 aa 600-650 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P18054
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Reactivity data
Select an application| IHC-P | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/50 | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
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Target data
Function
Catalyzes the regio and stereo-specific incorporation of molecular oxygen into free and esterified polyunsaturated fatty acids generating lipid hydroperoxides that can be further reduced to the corresponding hydroxy species (PubMed:17493578, PubMed:18311922, PubMed:1851637, PubMed:32404334, PubMed:8319693, PubMed:8500694). Mainly converts arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) to the specific bioactive lipid (12S)-hydroperoxyeicosatetraenoate/(12S)-HPETE (PubMed:17493578, PubMed:22984144, PubMed:24282679, PubMed:8319693, PubMed:8500694). Through the production of bioactive lipids like (12S)-HPETE it regulates different biological processes including platelet activation (PubMed:8319693, PubMed:8500694). It can also catalyze the epoxidation of double bonds of polyunsaturated fatty acids such as (14S)-hydroperoxy-docosahexaenoate/(14S)-HPDHA resulting in the formation of (13S,14S)-epoxy-DHA (PubMed:23504711). Furthermore, it may participate in the sequential oxidations of DHA ((4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate) to generate specialized pro-resolving mediators (SPMs) like resolvin D5 ((7S,17S)-diHPDHA) and (7S,14S)-diHPDHA, that actively down-regulate the immune response and have anti-aggregation properties with platelets (PubMed:32404334). An additional function involves a multistep process by which it transforms leukotriene A4/LTA4 into the bioactive lipids lipoxin A4/LXA4 and lipoxin B4/LXB4, both are vasoactive and LXA4 may regulate neutrophil function via occupancy of specific recognition sites (PubMed:8250832). Can also peroxidize linoleate ((9Z,12Z)-octadecadienoate) to (13S)-hydroperoxyoctadecadienoate/ (13S-HPODE) (By similarity). Due to its role in regulating both the expression of the vascular endothelial growth factor (VEGF, an angiogenic factor involved in the survival and metastasis of solid tumors) and the expression of integrin beta-1 (known to affect tumor cell migration and proliferation), it can be regarded as protumorigenic (PubMed:16638750, PubMed:22237009, PubMed:9751607). Important for cell survival, as it may play a role not only in proliferation but also in the prevention of apoptosis in vascular smooth muscle cells (PubMed:23578768).
Alternative names
12LO, LOG12, ALOX12, Polyunsaturated fatty acid lipoxygenase ALOX12, Arachidonate (12S)-lipoxygenase, Arachidonate (15S)-lipoxygenase, Linoleate (13S)-lipoxygenase, Lipoxin synthase 12-LO, Platelet-type lipoxygenase 12, 12S-LOX, 12S-lipoxygenase
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Rabbit Polyclonal 12 Lipoxygenase/ALOX12 antibody. C-terminal. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human ALOX12 aa 600-650 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ALOX12 aa 600-650 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P18054
- Purification technique
- Precipitation Ammonium Sulphate
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
12-Lipoxygenase (12-LOX) also known as ALOX12 is an enzyme that catalyzes the oxygenation of arachidonic acid to produce 12-hydroxyeicosatetraenoic acid (12-HETE). It has a molecular mass of approximately 75 kDa. This enzyme is widely expressed in various tissues including platelets lungs and pancreatic tissue. By converting polyunsaturated fatty acids such as arachidonic acid into bioactive lipids 12-LOX plays an important role in regulating cellular and physiological functions.
- Biological function summary
12-LOX influences many cellular processes such as inflammation and thrombosis regulation. It is part of the lipoxygenase pathway synthesizing lipid signaling molecules that modulate immune responses and cellular growth. The enzyme does not form part of a large protein complex but interacts with lipid membranes where it exerts its catalytic function. Through its activity 12-LOX contributes to the production of eicosanoids which are signaling molecules with various physiological roles.
- Pathways
12-LOX is involved in the arachidonic acid metabolism pathway and the eicosanoid signaling pathway. These pathways play roles in inflammation and immune response modulation. It works alongside other related lipoxygenases such as ALOX5 which produce different but related signaling molecules. The interaction between these enzymes helps maintain a balanced production of inflammatory mediators which controls cell proliferation and responses to external stimuli.
- Associated diseases and disorders
Alterations in 12-LOX expression or function associate strongly with cardiovascular diseases and various types of cancers. In cardiovascular disease 12-LOX-derived eicosanoids contribute to the development of atherosclerosis by promoting inflammation and endothelial dysfunction. In cancer the dysregulation of 12-LOX can lead to increased tumor growth and metastasis. These conditions often exhibit altered interactions with proteins such as COX-2 another enzyme involved in eicosanoid production highlighting the role of 12-LOX in complex disease mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-12 Lipoxygenase/ALOX12 antibody - C-terminal (ab211506)
All lanes: Western blot - Anti-12 Lipoxygenase/ALOX12 antibody - C-terminal (ab211506) at 1/1000 dilution
All lanes: K562 cell lysate at 35 µg
Predicted band size: 76 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-12 Lipoxygenase/ALOX12 antibody - C-terminal (ab211506)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human heart tissue, labeling 12 Lipoxygenase/ALOX12 using ab211506 at a 1/50 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-12 Lipoxygenase/ALOX12 antibody - C-terminal (ab211506)
Immunofluorescence analysis of A549 cells, labeling 12 Lipoxygenase/ALOX12 using ab211506 at a 1/100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1/400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).
Flow Cytometry (Intracellular) - Anti-12 Lipoxygenase/ALOX12 antibody - C-terminal (ab211506)
Intracellular Flow Cytometry analysis of K562 cells labeling 12 Lipoxygenase/ALOX12 (bottom histogram), using ab211506 at a 1/10 dilution, and negative control cells (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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