AB237807

Anti-14-3-3 alpha + beta antibody

Name: Anti-14-3-3 alpha + beta antibody (ab237807) | Abcam
Brand: Abcam Limited
SKU: AB237807
Price: 510 USD
Availability: InStock

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Polyclonal 1433B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat, Zebrafish samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human YWHAB.

View Alternative Names

14-3-3 protein beta/alpha, Protein 1054, Protein kinase C inhibitor protein 1, KCIP-1, YWHAB

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 alpha + beta antibody (AB237807)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 alpha + beta antibody (AB237807)

Paraffin-embedded human small intestine tissue stained for 14-3-3 alpha + beta using ab237807 at 1/400 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 alpha + beta antibody (AB237807)

Paraffin-embedded human kidney tissue stained for 14-3-3 alpha + beta using ab237807 at 1/600 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 alpha + beta antibody (AB237807)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 alpha + beta antibody (AB237807)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for 14-3-3 alpha + beta (green) using ab237807 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488^® conjugated Goat Anti-Rabbit IgG (H+L).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 alpha + beta antibody (AB237807)

Paraffin-embedded human testis tissue stained for 14-3-3 alpha + beta using ab237807 at 1/600 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-14-3-3 alpha + beta antibody (AB237807)

All lanes:

Western blot - Anti-14-3-3 alpha + beta antibody (ab237807) at 1/500 dilution

Lane 1:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Lane 2:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 28 kDa

false

Supplier Data

Western blot - Anti-14-3-3 alpha + beta antibody (AB237807)

All lanes:

Western blot - Anti-14-3-3 alpha + beta antibody (ab237807) at 1/500 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 2:

Raji (human Burkitt's lymphoma cell line) whole cell lysate

Lane 3:

Mouse liver lysate

Lane 4:

Mouse brain lysate

Lane 5:

Rat liver lysate

Lane 6:

Rat lung lysate

Lane 7:

Mouse lung lysate

Lane 8:

Zebrafish lysate

Secondary

All lanes:

Goat polyclonal to Rabbit IgG at 1/10000 dilution

Predicted band size: 28 kDa

false

Supplier Data

Western blot - Anti-14-3-3 alpha + beta antibody (AB237807)

All lanes:

Western blot - Anti-14-3-3 alpha + beta antibody (ab237807) at 1/500 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 2:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Lane 3:

Raji (human Burkitt's lymphoma cell line) whole cell lysate

Lane 4:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 5:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Lane 6:

HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate

Lane 7:

Rat brain lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 28 kDa

false

Supplier Data

Western blot - Anti-14-3-3 alpha + beta antibody (AB237807)

All lanes:

Western blot - Anti-14-3-3 alpha + beta antibody (ab237807) at 1/500 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 2:

EC109 (Human esophageal squamous cell carcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to Rabbit IgG at 1/15000 dilution

Predicted band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human, Zebrafish

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human YWHAB.

P31946

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/500", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity greater than 95%.

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

14-3-3 alpha + beta proteins also known as YWHAZ (14-3-3 zeta) and YWHAB (14-3-3 beta) are part of a family of conserved proteins weighing approximately 28-33 kDa each. These proteins are found in many tissues and engage in binding phosphorylated serine/threonine residues on their target molecules. This binding influences the activity localization and stability of these target proteins. Expression of 14-3-3 alpha + beta proteins is prominent in the brain where they participate in key signaling pathways.

Biological function summary

14-3-3 proteins play central roles in regulating diverse cellular processes. These proteins frequently serve as scaffolding molecules stabilizing complexes for signal transduction pathways. By interacting with numerous partner proteins 14-3-3 complexes modulate cell cycle control apoptosis and DNA damage response. They help maintain cellular homeostasis by binding and regulating enzymes receptors and transcription factors.

Pathways

14-3-3 proteins are integral to many signaling cascades. The MAPK/ERK pathway involves these proteins with 14-3-3 binding to Raf kinases influencing signal transduction. They also participate in the PI3K/Akt pathway where 14-3-3 interaction with Akt affects cell survival and growth. 14-3-3 proteins regulate various cellular functions by interacting with related kinases and phosphatases in these pathways.

Alterations of 14-3-3 protein function have significant implications. Neurodegenerative disorders such as Alzheimer’s disease show changes in 14-3-3 protein binding patterns affecting neuronal stability. Aberrant 14-3-3 interactions also connect to cancerous pathways particularly in breast cancer where regulation of p53 and other tumor suppressor proteins becomes disrupted. 14-3-3 proteins influence disease progression through their extensive network of protein interactions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13.

See full target information YWHAB

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell & bioscience 13:45 PubMed36879298

2023

PABPN1 regulates mRNA alternative polyadenylation to inhibit bladder cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Chen,Wei Dong,Menghao Zhou,Chenlu Yang,Ming Xiong,Gallina Kazobinka,Zhaohui Chen,Yifei Xing,Teng Hou

Cell transplantation 30:963689720983786 PubMed33588586

2021

Circ SMARCA5 Inhibited Tumor Metastasis by Interacting with SND1 and Downregulating the YWHAB Gene in Cervical Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xia Zhang,Qing Zhang,Ke Zhang,Fang Wang,Xiaogai Qiao,Jinquan Cui

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-14-3-3 alpha + beta antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 alpha + beta antibody (AB237807)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 alpha + beta antibody (AB237807)

Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 alpha + beta antibody (AB237807)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 alpha + beta antibody (AB237807)

Western blot - Anti-14-3-3 alpha + beta antibody (AB237807)

Western blot - Anti-14-3-3 alpha + beta antibody (AB237807)

Western blot - Anti-14-3-3 alpha + beta antibody (AB237807)

Western blot - Anti-14-3-3 alpha + beta antibody (AB237807)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise