AB125032

Anti-14-3-3 antibody [EPR6380]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal 1433B phospho S184 + S186 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

14-3-3 protein beta/alpha, Protein 1054, Protein kinase C inhibitor protein 1, KCIP-1, YWHAB

2 Images

Unknown

Western blot - Anti-14-3-3 antibody [EPR6380] (AB125032)

All lanes:

Western blot - Anti-14-3-3 antibody [EPR6380] (ab125032) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

SH-SY5Y cell lysate at 10 µg

Lane 3:

Raji cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti rabbit at 1/2000 dilution

Predicted band size: 28 kDa

Observed band size: 27 kDa

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Supplier Data

Western blot - Anti-14-3-3 antibody [EPR6380] (AB125032)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-14-3-3 antibody [EPR6380] (ab125032) at 1/1000 dilution

Lane 1:

14-3-3 beta (YWHAB) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Lane 2:

14-3-3 gamma (YWHAG) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Lane 3:

14-3-3 epsilon (YWHAE) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Lane 4:

14-3-3 zeta (YWHAZ) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Lane 5:

14-3-3 eta (YWHAH) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Lane 6:

14-3-3 theta (YWHAQ) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Lane 7:

14-3-3 sigma (SFN) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Exposure time: 60s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6380

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 14-3-3 proteins are a family of regulatory molecules with a molecular mass around 28 to 32 kDa. These proteins exist in seven isoforms in humans termed beta gamma epsilon zeta eta theta and sigma. 14-3-3 proteins are ubiquitously expressed in eukaryotic cells including neurons liver cells and immune cells. These proteins participate in modulation of many cellular processes by binding to phosphorylated serine and threonine residues on various target proteins. This binding alters the conformation of target proteins influencing their activity subcellular localization or stability.

Biological function summary

14-3-3 proteins are involved in signal transduction cell cycle control and apoptosis regulation. They participate in protein interactions and form dimers that serve as scaffolds assembling complexes and linking signaling pathways. The proteins associate with kinases such as Raf-1 thereby contributing to signaling pathways that include MAPK/ERK. At the cellular level 14-3-3 proteins govern key mechanisms like the G1/S phase transition and stress response which are critical for maintaining cellular homeostasis.

Pathways

14-3-3 proteins play critical roles in the PI3K/AKT and MAPK/ERK pathways. They interact with several key proteins within these pathways including AKT and Raf-1 mediating cellular responses to growth factors and stress. In the PI3K/AKT pathway 14-3-3 proteins help regulate the progression of the cell cycle and promote cell survival linking extracellular signals to the machinery that governs cell fate. These interactions underline the role of 14-3-3 proteins in integrating and regulating complex cell signaling networks.

14-3-3 proteins are associated with neurodegenerative diseases like Alzheimer's and certain types of cancer. In Alzheimer's disease 14-3-3 proteins interact with tau protein contributing to the pathological aggregation of tau associated with neuronal damage. Similarly in various cancers their interaction with cell cycle regulators like p53 can influence tumor progression by affecting cell division and apoptosis. These connections highlight the involvement of 14-3-3 proteins in disease mechanisms making them potential targets for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13.

See full target information YWHAB phospho S184 + S186

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International immunopharmacology 138:112546 PubMed38917522

2024

MC1R regulates T regulatory cell differentiation through metabolic reprogramming to promote colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shaoliang Zhu,Mengjie Zou,Chunxing Li,Yuntian Tang,Honglin Luo,Xiaofeng Dong

The EMBO journal 42:e113590 PubMed38073509

2023

Extracellular vesicles and co-isolated endogenous retroviruses from murine cancer cells differentially affect dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Federico Cocozza,Lorena Martin-Jaular,Lien Lippens,Aurelie Di Cicco,Yago A Arribas,Nicolas Ansart,Florent Dingli,Michael Richard,Louise Merle,Mabel Jouve San Roman,Patrick Poullet,Damarys Loew,Daniel Lévy,An Hendrix,George Kassiotis,Alain Joliot,Mercedes Tkach,Clotilde Théry

Thoracic cancer 14:1831-1842 PubMed37192740

2023

Circ_0069094 regulates malignant phenotype and paclitaxel resistance in breast cancer cells via targeting the miR-136-5p/YWHAZ axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhihua Kong,Qi Han,Bisheng Zhu,Long Wan,Enrong Feng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-14-3-3 antibody [EPR6380]

Western blot - Anti-14-3-3 antibody [EPR6380] (AB125032)

Western blot - Anti-14-3-3 antibody [EPR6380] (AB125032)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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