JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB314175

Anti-14-3-3 beta antibody [6F6]

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal 1433B antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human YWHAB.

View Alternative Names

14-3-3 protein beta/alpha, Protein 1054, Protein kinase C inhibitor protein 1, KCIP-1, YWHAB, 14-3-3 protein beta/alpha, Prepronerve growth factor RNH-1, Protein kinase C inhibitor protein 1, KCIP-1, Ywhab, 14-3-3 protein beta/alpha, Protein kinase C inhibitor protein 1, KCIP-1, Ywhab

2 Images
Flow Cytometry - Anti-14-3-3 beta antibody [6F6] (AB314175)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-14-3-3 beta antibody [6F6] (AB314175)

Overlay Peak curve showing HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained with ab314175 (pink line) at 1 : 100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1x10E6cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1 : 200 dilution for 35min at 4°C. Control antibody (green line) was rabbit IgG (1ug/1x10E6cells) used under the same conditions. Acquisition of >10,000 events was performed.

Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 beta antibody [6F6] (AB314175)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 beta antibody [6F6] (AB314175)

Immunofluorescence staining of PC-3 (Human prostate adenocarcinoma cell line) cells with ab314175 at 1 : 50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor® 494-conjugated Goat Anti-Rabbit IgG(H+L).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

6F6

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human YWHAB.

P31946

style
left-column

Complementary Products

Primary Antibodies

AB259976

Anti-Synaptopodin antibody [EPR23922-79]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Multiplex immunohistochemistry - Anti-Synaptopodin antibody [EPR23922-79] (AB259976)

  • 0
  • 0
Primary Antibodies

AB187027

Anti-RhoA antibody [EPR18134]

20ul selling size|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-RhoA antibody [EPR18134] (AB187027)

  • 4
  • 1
Primary Antibodies

AB108198

Anti-alpha Actinin 4 antibody [EPR2533(2)] - Loading Control

20ul selling size|RabMAb|Recombinant|KO Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-alpha Actinin 4 antibody [EPR2533(2)] - Loading Control (AB108198)

  • 5
  • 9
Primary Antibodies

AB248786

Anti-14-3-3 gamma/YWHAG antibody [EPR9654(B)] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Western blot - Anti-14-3-3 gamma/YWHAG antibody [EPR9654(B)] - BSA and Azide free (AB248786)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50 - 1/200", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
style
left-column

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

14-3-3 beta also known as YWHAB is a protein that electrically neutralizes and stabilizes partner proteins. It has a mass of about 30 kDa and is part of the highly conserved 14-3-3 protein family. This family is ubiquitous in eukaryotic cells with 14-3-3 beta being expressed in many tissues including the brain and muscles. Its mechanical role involves binding phosphorylated serine and threonine residues which influences the conformation and function of the target protein.
Biological function summary

14-3-3 beta plays a central role in cellular processes like signal transduction apoptosis and cell cycle control. It often acts in concert with other proteins forming complexes that regulate these vital functions. For instance it binds to Raf-1 kinase affecting cell growth and differentiation. It also interacts with proteins involved in the mitogen-activated protein kinase (MAPK) pathway highlighting its diverse biological significance.

Pathways

14-3-3 beta is important in both the MAPK and apoptotic pathways. In the MAPK pathway it helps regulate the phosphorylation status of proteins involved in cell proliferation. The protein binds to Raf-1 an important component in MAPK signaling impacting cellular responses to growth signals. In the context of apoptosis 14-3-3 beta partners with BAD protein a pro-apoptotic factor sequestering it away from mitochondria this interaction is critical for cell survival by preventing the apoptotic process.

Disruptions in 14-3-3 beta function associate with neurodegenerative diseases and cancer. For example its interaction with proteins like Tau connected to Alzheimer's disease points to its involvement in neurodegeneration. Aberrations in its regulatory roles can also contribute to uncontrolled cell growth linked to cancer. Furthermore its interaction with Bcl-2 family proteins ties it to variations in apoptotic regulation seen in cancerous cells.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13.
See full target information YWHAB

Additional targets

Ywhab,Ywhab
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 12:217-221 PubMed27347128

2016

Downregulation of SENP1 inhibits cell proliferation, migration and promotes apoptosis in human glioma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qiu-Sheng Zhang,Meng Zhang,Xian-Jian Huang,Xiao-Jia Liu,Wei-Ping Li
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com