AB15260

Anti-14-3-3 beta antibody

Name: Anti-14-3-3 beta antibody (ab15260) | Abcam
Brand: Abcam Limited
SKU: AB15260
Price: 565 USD
Availability: InStock
Rating: 5 (3 reviews)

(3 Reviews)

(7 Publications)

Rabbit Polyclonal 1433B antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 7 publications.

View Alternative Names

14-3-3 protein beta/alpha, Protein 1054, Protein kinase C inhibitor protein 1, KCIP-1, YWHAB

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 beta antibody (AB15260)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 beta antibody (AB15260)

ab15260 staining 14-3-3 beta in human brain by Immunohistochemistry (FFPE-sections).

Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 beta antibody (AB15260)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 beta antibody (AB15260)

ICC/IF image of ab15260 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15260, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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Western blot - Anti-14-3-3 beta antibody (AB15260)

All lanes:

Western blot - Anti-14-3-3 beta antibody (ab15260) at 1/25 dilution

All lanes:

HeLa cell lysate

Predicted band size: 28 kDa

Observed band size: 28 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

14-3-3 beta also known as YWHAB is a protein that electrically neutralizes and stabilizes partner proteins. It has a mass of about 30 kDa and is part of the highly conserved 14-3-3 protein family. This family is ubiquitous in eukaryotic cells with 14-3-3 beta being expressed in many tissues including the brain and muscles. Its mechanical role involves binding phosphorylated serine and threonine residues which influences the conformation and function of the target protein.

Biological function summary

14-3-3 beta plays a central role in cellular processes like signal transduction apoptosis and cell cycle control. It often acts in concert with other proteins forming complexes that regulate these vital functions. For instance it binds to Raf-1 kinase affecting cell growth and differentiation. It also interacts with proteins involved in the mitogen-activated protein kinase (MAPK) pathway highlighting its diverse biological significance.

Pathways

14-3-3 beta is important in both the MAPK and apoptotic pathways. In the MAPK pathway it helps regulate the phosphorylation status of proteins involved in cell proliferation. The protein binds to Raf-1 an important component in MAPK signaling impacting cellular responses to growth signals. In the context of apoptosis 14-3-3 beta partners with BAD protein a pro-apoptotic factor sequestering it away from mitochondria this interaction is critical for cell survival by preventing the apoptotic process.

Disruptions in 14-3-3 beta function associate with neurodegenerative diseases and cancer. For example its interaction with proteins like Tau connected to Alzheimer's disease points to its involvement in neurodegeneration. Aberrations in its regulatory roles can also contribute to uncontrolled cell growth linked to cancer. Furthermore its interaction with Bcl-2 family proteins ties it to variations in apoptotic regulation seen in cancerous cells.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13.

See full target information YWHAB

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

PNAS nexus 2:pgad420 PubMed38130664

2023

Regulation of lipolysis by 14-3-3 proteins on human adipocyte lipid droplets.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Yang,Zinger Yang Loureiro,Anand Desai,Tiffany DeSouza,Kaida Li,Hui Wang,Sarah M Nicoloro,Javier Solivan-Rivera,Silvia Corvera

Nature communications 14:7933 PubMed38040731

2023

A simple method for developing lysine targeted covalent protein reagents.

Applications

Unspecified application

Species

Unspecified reactive species

Ronen Gabizon,Barr Tivon,Rambabu N Reddi,Maxime C M van den Oetelaar,Hadar Amartely,Peter J Cossar,Christian Ottmann,Nir London

eNeuro 10: PubMed36898832

2023

Characterization of the Tau Interactome in Human Brain Reveals Isoform-Dependent Interaction with 14-3-3 Family Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Ryan K Betters,Emma Luhmann,Amy C Gottschalk,Zhen Xu,Mallory R Shin,Christopher P Ptak,Kimberly L Fiock,Lilliana C Radoshevich,Marco M Hefti

The Journal of biological chemistry 298:102513 PubMed36150501

2022

Serine 13 of the human cytomegalovirus viral cyclin-dependent kinase UL97 is required for regulatory protein 14-3-3 binding and UL97 stability.

Applications

Unspecified application

Species

Unspecified reactive species

Satoko Iwahori,Angie C Umaña,Robert F Kalejta,Takayuki Murata

Acta neuropathologica 143:453-469 PubMed35141810

2022

Brain region-specific susceptibility of Lewy body pathology in synucleinopathies is governed by α-synuclein conformations.

Applications

Unspecified application

Species

Unspecified reactive species

Laura de Boni,Aurelia Hays Watson,Ludovica Zaccagnini,Amber Wallis,Kristina Zhelcheska,Nora Kim,John Sanderson,Haiyang Jiang,Elodie Martin,Adam Cantlon,Matteo Rovere,Lei Liu,Marc Sylvester,Tammaryn Lashley,Ulf Dettmer,Zane Jaunmuktane,Tim Bartels

Experimental and therapeutic medicine 15:2381-2387 PubMed29467845

2018

14-3-3β exerts glioma-promoting effects and is associated with malignant progression and poor prognosis in patients with glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Liu,Zhixiong Liu,Hao Wang,Long Chen,Fuqiang Ruan,Jihui Zhang,Yi Hu,Hengshan Luo,Shuai Wen

Neuro-degenerative diseases 9:139-44 PubMed22213780

2012

Desmoplakin as a potential candidate for cerebrospinal fluid marker to rule out 14-3-3 false positive rates in sporadic Creutzfeldt-Jakob disease differential diagnosis.

Applications

Species

Human

Joanna Gawinecka,Barbara Ciesielczyk,Pascual Sanchez-Juan,Matthias Schmitz,Uta Heinemann,Inga Zerr

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-14-3-3 beta antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 beta antibody (AB15260)

Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 beta antibody (AB15260)

Western blot - Anti-14-3-3 beta antibody (AB15260)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise