Rabbit Polyclonal 1433B phospho S184 + S186 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human YWHAB phospho S184 + S186 aa 1-150.
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- Journal of Cancer 11:858-8662020MiR-129-5p sensitization of lung cancer cells to etoposide-induced apoptosis by reducing YWHAB.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChengshan Xu et. al.PubMed 31949489
- Frontiers in medicine 6:2372019Low Expression of 14-3-3beta Is Associated With Adverse Survival of Diffuse Large B-Cell Lymphoma Patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChaoping Li et. al.PubMed 31737636
- Molecular medicine reports 17:2493-25002017Downregulation of 14-3-3β inhibits proliferation and migration in osteosarcoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQuanming Wu,Jianwei Zhu,Fan Liu,Jin Liu,Mingpeng LiPubMed 29207109
- Molecular & cellular proteomics : MCP 11:M111.0095302011Brainstem deficiency of the 14-3-3 regulator of serotonin synthesis: a proteomics analysis in the sudden infant death syndrome.Applications:WBReactive species:HumanKevin G Broadbelt,Keith D Rivera,David S Paterson,Jhodie R Duncan,Felicia L Trachtenberg,Joao A Paulo,Martha D Stapels,Natalia S Borenstein,Richard A Belliveau,Elisabeth A Haas,Christina Stanley,Henry F Krous,Hanno Steen,Hannah C KinneyPubMed 21976671
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human YWHAB phospho S184 + S186 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link P31946
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Expected | Tested | Expected |
| Pig | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/5000 - 1/20000 | Notes - |
Species Human | Dilution info 1/5000 - 1/20000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100 - 1/1000 | Notes - |
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/200 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
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Target data
Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13.
Alternative names
14-3-3 protein beta/alpha, Protein 1054, Protein kinase C inhibitor protein 1, KCIP-1, YWHAB
Recommended products
Rabbit Polyclonal 1433B phospho S184 + S186 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human YWHAB phospho S184 + S186 aa 1-150.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human YWHAB phospho S184 + S186 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link P31946
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
14-3-3 beta also known as YWHAB is a protein that electrically neutralizes and stabilizes partner proteins. It has a mass of about 30 kDa and is part of the highly conserved 14-3-3 protein family. This family is ubiquitous in eukaryotic cells with 14-3-3 beta being expressed in many tissues including the brain and muscles. Its mechanical role involves binding phosphorylated serine and threonine residues which influences the conformation and function of the target protein.
- Biological function summary
14-3-3 beta plays a central role in cellular processes like signal transduction apoptosis and cell cycle control. It often acts in concert with other proteins forming complexes that regulate these vital functions. For instance it binds to Raf-1 kinase affecting cell growth and differentiation. It also interacts with proteins involved in the mitogen-activated protein kinase (MAPK) pathway highlighting its diverse biological significance.
- Pathways
14-3-3 beta is important in both the MAPK and apoptotic pathways. In the MAPK pathway it helps regulate the phosphorylation status of proteins involved in cell proliferation. The protein binds to Raf-1 an important component in MAPK signaling impacting cellular responses to growth signals. In the context of apoptosis 14-3-3 beta partners with BAD protein a pro-apoptotic factor sequestering it away from mitochondria this interaction is critical for cell survival by preventing the apoptotic process.
- Associated diseases and disorders
Disruptions in 14-3-3 beta function associate with neurodegenerative diseases and cancer. For example its interaction with proteins like Tau connected to Alzheimer's disease points to its involvement in neurodegeneration. Aberrations in its regulatory roles can also contribute to uncontrolled cell growth linked to cancer. Furthermore its interaction with Bcl-2 family proteins ties it to variations in apoptotic regulation seen in cancerous cells.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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7 product images
Western blot - Anti-14-3-3 beta antibody (ab97273)
All lanes: Western blot - Anti-14-3-3 beta antibody (ab97273) at 1/10000 dilution
Lane 1: 293T whole cell lysate at 30 µg
Lane 2: A431 whole cell lysate at 30 µg
Lane 3: HeLa whole cell lysate at 30 µg
Lane 4: HepG2 whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 28 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 beta antibody (ab97273)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of SW480 xenograft labelling 14-3-3 beta with ab97273 at a dilution of 1/500.
Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 beta antibody (ab97273)
ab97273, at a 1/200 dilution, staining 14-3-3 beta in paraformaldehyde fixed HeLa by Immunofluorescence analysis.
Right image is merged with DNA probe.
Western blot - Anti-14-3-3 beta antibody (ab97273)
12% SDS PAGE.
All lanes: Western blot - Anti-14-3-3 beta antibody (ab97273) at 1/10000 dilution
All lanes: Mouse brain tissue lysate at 20 µg
Predicted band size: 28 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 beta antibody (ab97273)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cervix labelling 14-3-3 beta with ab97273 at a dilution of 1/500.
Western blot - Anti-14-3-3 beta antibody (ab97273)
12% SDS PAGE
All lanes: Western blot - Anti-14-3-3 beta antibody (ab97273) at 1/10000 dilution
Lane 1: A431 whole cell lysate at 30 µg
Lane 2: H1299 whole cell lysate at 30 µg
Lane 3: HeLa whole cell lysate at 30 µg
Lane 4: HepG2 whole cell lysate at 30 µg
Lane 5: MOLT4 whole cell lysate at 30 µg
Lane 6: Raji whole cell lysate at 30 µg
Predicted band size: 28 kDa
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-14-3-3 beta antibody (ab97273)
14-3-3 beta western blot using anti-14-3-3 beta antibody ab97273. Publication image and figure legend from Xu, C., Du, Z., et al., 2020, J Cancer, PubMed 31949489.
ab97273 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab97273 please see the product overview.
YWHAB is a direct downstream target of miR-129-5p. Note: (A) The potential interaction between miR-129-5p and putative binding sites in the YWHAB 3'-UTR predicted by TargetScan (upper panel). A highly-conserved miR-129-5p targeting sequence was predicted in the 3'-UTR of the YWHAB mRNA (bottom panel). (B) The sequences of the mutated YWHAB 3' UTR, in which several nucleotides within the miR-129-5p binding site were mutated. (C) The luciferase activity in the A549 and H1299 cells. Cells were cotransfected with miRNAs and luciferase report plasmids and the luciferase activities were detected in different groups. Each value is evaluated by the relative luciferase activity of firefly to renilla. (D) Effect of miR-129-5p on YWHAB mRNA level in the A549 and H1299 cells. After the cells were transfected, the YWHAB protein expression was detected by RT-PCR. β-actin was used for the internal control. (E) Effect of miR-129-5p on YWHAB protein level in the A549 and H1299 cells. After the cells were transfected, the YWHAB protein expression was detected by Western blot. GAPDH was used for the internal control. Relative levels of YWHAB were analyzed by quantification of the density of the bands with Image J software (bottom panel).
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