Anti-14-3-3 beta antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 beta antibody (AB97273)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of SW480 xenograft labelling 14-3-3 beta with ab97273 at a dilution of 1/500.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 beta antibody (AB97273)

ab97273, at a 1/200 dilution, staining 14-3-3 beta in paraformaldehyde fixed HeLa by Immunofluorescence analysis.
Right image is merged with DNA probe.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 beta antibody (AB97273)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cervix labelling 14-3-3 beta with ab97273 at a dilution of 1/500.

• WB

Unknown

Western blot - Anti-14-3-3 beta antibody (AB97273)

12% SDS PAGE

All lanes:

Western blot - Anti-14-3-3 beta antibody (ab97273) at 1/10000 dilution

Lane 1:

A431 whole cell lysate at 30 µg

Lane 2:

H1299 whole cell lysate at 30 µg

Lane 3:

HeLa whole cell lysate at 30 µg

Lane 4:

HepG2 whole cell lysate at 30 µg

Lane 5:

MOLT4 whole cell lysate at 30 µg

Lane 6:

Raji whole cell lysate at 30 µg

Predicted band size: 28 kDa

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• WB

Supplier Data

Western blot - Anti-14-3-3 beta antibody (AB97273)

All lanes:

Western blot - Anti-14-3-3 beta antibody (ab97273) at 1/10000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Lane 3:

HeLa whole cell lysate at 30 µg

Lane 4:

HepG2 whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 28 kDa

false

• WB

Supplier Data

Western blot - Anti-14-3-3 beta antibody (AB97273)

12% SDS PAGE.

All lanes:

Western blot - Anti-14-3-3 beta antibody (ab97273) at 1/10000 dilution

All lanes:

Mouse brain tissue lysate at 20 µg

Predicted band size: 28 kDa

false

• WB

CiteAb

Western blot - Anti-14-3-3 beta antibody (AB97273)

14-3-3 beta western blot using anti-14-3-3 beta antibody ab97273. Publication image and figure legend from Xu, C., Du, Z., et al., 2020, J Cancer, PubMed 31949489.

ab97273 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab97273 please see the product overview.

YWHAB is a direct downstream target of miR-129-5p. Note : (A) The potential interaction between miR-129-5p and putative binding sites in the YWHAB 3'-UTR predicted by TargetScan (upper panel). A highly-conserved miR-129-5p targeting sequence was predicted in the 3'-UTR of the YWHAB mRNA (bottom panel). (B) The sequences of the mutated YWHAB 3' UTR, in which several nucleotides within the miR-129-5p binding site were mutated. (C) The luciferase activity in the A549 and H1299 cells. Cells were cotransfected with miRNAs and luciferase report plasmids and the luciferase activities were detected in different groups. Each value is evaluated by the relative luciferase activity of firefly to renilla. (D) Effect of miR-129-5p on YWHAB mRNA level in the A549 and H1299 cells. After the cells were transfected, the YWHAB protein expression was detected by RT-PCR. β-actin was used for the internal control. (E) Effect of miR-129-5p on YWHAB protein level in the A549 and H1299 cells. After the cells were transfected, the YWHAB protein expression was detected by Western blot. GAPDH was used for the internal control. Relative levels of YWHAB were analyzed by quantification of the density of the bands with Image J software (bottom panel).

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