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AB111856

Anti-14-3-3 beta + zeta (phospho S184 + S186) antibody

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(1 Publication)

Rabbit Polyclonal 1433B phospho S184 antibody. Suitable for WB and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human YWHAB phospho S186.

View Alternative Names

14-3-3 protein beta/alpha, Protein 1054, Protein kinase C inhibitor protein 1, KCIP-1, YWHAB, 14-3-3 protein zeta/delta, Protein kinase C inhibitor protein 1, KCIP-1, YWHAZ

1 Images
Western blot - Anti-14-3-3 beta + zeta (phospho S184 + S186) antibody (AB111856)
  • WB

Unknown

Western blot - Anti-14-3-3 beta + zeta (phospho S184 + S186) antibody (AB111856)

All lanes:

Western blot - Anti-14-3-3 beta + zeta (phospho S184 + S186) antibody (ab111856) at 1/500 dilution

Lane 1:

RAW264.7 cell extracts, treated with UV (15mins) at 30 µg

Lane 2:

RAW264.7 cell extracts, treated with UV (15mins) at 30 µg with immunizing peptide

Predicted band size: 28 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

Synthetic Peptide within Human YWHAB phospho S186. The exact immunogen used to generate this antibody is proprietary information.

P31946

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Complementary Products

Primary Antibodies

AB206292

Anti-14-3-3 eta/YWHAH antibody [EPR16750]

RabMAb|Recombinant

Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (AB206292)

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Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Primary Antibodies

AB317439

Anti-14-3-3 zeta antibody [EPR27044-19] - BSA and Azide free

Recombinant|RabMAb

Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 zeta antibody [EPR27044-19] - BSA and Azide free (AB317439)

  • 0
  • 0
Primary Antibodies

AB182858

Anti-Bcl-2 antibody [EPR17509]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [EPR17509] (AB182858)

  • 5
  • 13
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab111856 was affinity-purified from Rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 14-3-3 beta and zeta proteins also known as YWHAB and YWHAZ respectively are part of the 14-3-3 protein family which typically includes proteins that are approximately 30 kDa in size. These proteins are highly expressed in the brain tissue and are found in other tissues too. The 14-3-3 proteins are known for their role in dimerization making them important in binding and stabilizing various phosphorylated client proteins. This dimerization allows them to act as scaffolding proteins facilitating the interaction and regulation of various signaling proteins.
Biological function summary

These proteins participate in a wide range of cellular processes mainly through their involvement in signal transduction pathways. They are an integral part of complex interactions within the cell often forming dimers to interact with phosphoserine-containing proteins. The 14-3-3 beta and zeta proteins play key roles in processes like apoptosis cell cycle control and cellular stress responses. Their ability to influence the activity of transcription factors and enzymes makes them important in maintaining cellular homeostasis.

Pathways

The 14-3-3 beta and zeta proteins are significant players in the MAPK and PI3K/AKT signaling pathways. These pathways are critical for regulating cell growth survival and apoptosis. They interact with proteins such as Raf-1 and Bad impacting kinase activities and apoptosis regulation. The role of 14-3-3 proteins as modulators and coordinators of these signaling cascades highlights their importance in cellular processes.

These proteins have associations with neurological and cancer-related diseases. In neurological contexts dysregulation of 14-3-3 proteins links to Alzheimer’s disease where aberrant interactions may contribute to tau phosphorylation anomalies. In cancer their interaction with key regulatory proteins like p53 impacts cellular proliferation and tumor suppression. These connections highlight the potential for 14-3-3 beta and zeta as therapeutic targets in various pathological states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13.
See full target information YWHAB phospho S184

Additional targets

14-3-3 protein zeta/delta phospho S186
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Acta pharmacologica Sinica 42:560-572 PubMed32694758

2020

Reduced intracellular chloride concentration impairs angiogenesis by inhibiting oxidative stress-mediated VEGFR2 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Li,Ying-Ying Liu,Xiao-Fei Lv,Zhuo-Miao Lin,Ting-Ting Zhang,Fei-Ran Zhang,Jia-Wei Guo,Yu Hong,Xiu Liu,Xiao-Chun Lin,Jia-Guo Zhou,Qian-Qian Wu,Si-Jia Liang,Jin-Yan Shang
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Product promise

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