AB315193

Anti-14-3-3 eta/YWHAH antibody [3G12]

Name: Anti-14-3-3 eta/YWHAH antibody [3G12] (ab315193) | Abcam
Brand: Abcam Limited
SKU: AB315193
Price: 580 USD
Availability: InStock

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Mouse Monoclonal 1433F antibody. Suitable for WB, ICC/IF and reacts with Human, Rat, Mouse samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human YWHAH.

View Alternative Names

YWHA1, YWHAH, 14-3-3 protein eta, Protein AS1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 eta/YWHAH antibody [3G12] (AB315193)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 eta/YWHAH antibody [3G12] (AB315193)

Immunofluorescent analysis of HeLa cells stained with ab315193, dilution 1 : 1,000 in red. Blue is DAPI staining of nuclear DNA. The ab315193 antibody reveals the diffuse cytoplasmic distribution of 14-3-3η protein with higher concentration in the perinuclear region.

Supplier Data

Western blot - Anti-14-3-3 eta/YWHAH antibody [3G12] (AB315193)

Western blot analysis of whole brain lysates (lanes 2,3), and cell lysates (lanes 4-8), using ab315193, dilution 1 : 5,000 in green : [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y, [8] C6 cells. Strong band at 28kDa corresponds to 14-3-3η protein, expressed in all preparations.

All lanes:

Western blot - Anti-14-3-3 eta/YWHAH antibody [3G12] (ab315193) at 1/5000 dilution

Lane 2:

Rat brain tissue lysate

Lane 3:

Mouse brain tissue lysate

Lane 4:

NIH-3T3 cell lysate

Lane 5:

HEK293 cell lysate

Lane 6:

HeLa cell lysate

Lane 7:

SH-SY5Y cell lysate

Lane 8:

C6 cell lysate

Observed band size: 28 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3G12

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human YWHAH.

Q04917

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7 - 8 Preservative: 0.03% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 14-3-3 eta protein also known by its gene name YWHAH functions mechanically as an adapter protein involved in many cellular processes. This protein weighs approximately 28 kDa and facilitates the modulation of signaling pathways by binding to phosphoserine-containing proteins. It exhibits widespread expression with significant presence in the brain emphasizing its role in nervous system activities. The 14-3-3 eta protein acts as a hub in various signal transduction pathways influencing cell cycle regulation and apoptosis.

Biological function summary

The 14-3-3 eta protein interacts with diverse intracellular proteins forming various complexes that influence cellular functions. It plays a pivotal role in cellular stress responses and the regulation of several metabolic processes. The protein modulates activity by altering the localization of target proteins or masking their phosphorylation sites which affects their function and stability. This regulatory capacity emphasizes the protein's impact on cell survival and metabolism.

Pathways

The 14-3-3 eta protein integrates into key signaling networks such as the MAPK/ERK and PI3K/AKT pathways. These pathways are essential in mediating processes like cell proliferation differentiation and survival. Within these pathways 14-3-3 eta functions alongside proteins like RAF1 and AKT1 coordinating cellular responses to external stimuli. Its involvement in these pathways highlights the adaptability and versatile roles it plays in cellular signaling and homeostasis.

The 14-3-3 eta protein has associations with conditions such as rheumatoid arthritis and neurodegenerative diseases. In rheumatoid arthritis altered expression and interactions with other proteins like cytokines contribute to inflammatory processes. In neurodegenerative disorders the dysregulation of 14-3-3 eta affects proteins related to tau pathology influencing disease progression. Understanding these connections helps in comprehending the protein's role in disease mechanisms and developing therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.

See full target information YWHAH

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-14-3-3 eta/YWHAH antibody [3G12]

Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 eta/YWHAH antibody [3G12] (AB315193)

Western blot - Anti-14-3-3 eta/YWHAH antibody [3G12] (AB315193)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise