AB206292

Anti-14-3-3 eta/YWHAH antibody [EPR16750]

Name: Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292) | Abcam
Brand: Abcam Limited
SKU: AB206292
Price: 465 USD
Availability: InStock
Rating: 5 (2 reviews)

RabMAb
Recombinant
What is this?

(2 Reviews)

(9 Publications)

Rabbit Recombinant Monoclonal 1433F antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 9 publications.

View Alternative Names

YWHA1, YWHAH, 14-3-3 protein eta, Protein AS1

6 Images

Supplier Data

Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (AB206292)

Negative control : 14-3-3 eta/YWHAH was immunoprecipitated from 1mg of Human brain whole cell lysate with ab206292 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab137106 (which is specific to 14-3-3 gamma) at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : Human brain whole cell lysate 10ug (Input). Lane 2 : ab206292 IP in Human brain whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab206292 in Human brain whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)

Predicted band size: 28 kDa

false

Supplier Data

Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (AB206292)

14-3-3 eta/YWHAH was immunoprecipitated from 1mg of Human brain whole cell lysate with ab206292 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab206292 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)

Predicted band size: 28 kDa

false

Supplier Data

Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (AB206292)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 15 seconds; Lane 2 and 3 : 3 minutes.

All lanes:

Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292) at 1/5000 dilution

Lane 1:

Human fetal brain tissue lysate at 20 µg

Lane 2:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

Lane 3:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Supplier Data

Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (AB206292)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292) at 1/2000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) cytosolic fraction at 10 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) nuclear fraction at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (AB206292)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292) at 1000 Da

Lane 1:

14-3-3 beta (YWHAB) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Lane 2:

14-3-3 gamma (YWHAG) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Lane 3:

14-3-3 epsilon (YWHAE) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Lane 4:

14-3-3 zeta (YWHAZ) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Lane 5:

14-3-3 eta (YWHAH) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Lane 6:

14-3-3 theta (YWHAQ) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Lane 7:

14-3-3 sigma (SFN) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Exposure time: 100s

Supplier Data

Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (AB206292)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1- 6 : 30 seconds; Lane 7 -10 : 3 minutes.

All lanes:

Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Mouse spleen tissue lysate at 10 µg

Lane 3:

Rat brain tissue lysate at 10 µg

Lane 4:

Rat spleen tissue lysate at 10 µg

Lane 5:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

Lane 6:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Lane 7:

Mouse heart tissue lysate at 10 µg

Lane 8:

Mouse kidney tissue lysate at 10 µg

Lane 9:

Rat heart tissue lysate at 10 µg

Lane 10:

Rat kidney tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Carrier free

Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16750

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 14-3-3 eta protein also known by its gene name YWHAH functions mechanically as an adapter protein involved in many cellular processes. This protein weighs approximately 28 kDa and facilitates the modulation of signaling pathways by binding to phosphoserine-containing proteins. It exhibits widespread expression with significant presence in the brain emphasizing its role in nervous system activities. The 14-3-3 eta protein acts as a hub in various signal transduction pathways influencing cell cycle regulation and apoptosis.

Biological function summary

The 14-3-3 eta protein interacts with diverse intracellular proteins forming various complexes that influence cellular functions. It plays a pivotal role in cellular stress responses and the regulation of several metabolic processes. The protein modulates activity by altering the localization of target proteins or masking their phosphorylation sites which affects their function and stability. This regulatory capacity emphasizes the protein's impact on cell survival and metabolism.

Pathways

The 14-3-3 eta protein integrates into key signaling networks such as the MAPK/ERK and PI3K/AKT pathways. These pathways are essential in mediating processes like cell proliferation differentiation and survival. Within these pathways 14-3-3 eta functions alongside proteins like RAF1 and AKT1 coordinating cellular responses to external stimuli. Its involvement in these pathways highlights the adaptability and versatile roles it plays in cellular signaling and homeostasis.

The 14-3-3 eta protein has associations with conditions such as rheumatoid arthritis and neurodegenerative diseases. In rheumatoid arthritis altered expression and interactions with other proteins like cytokines contribute to inflammatory processes. In neurodegenerative disorders the dysregulation of 14-3-3 eta affects proteins related to tau pathology influencing disease progression. Understanding these connections helps in comprehending the protein's role in disease mechanisms and developing therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.

See full target information YWHAH

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Discover oncology 16:72 PubMed39836297

2025

Anti-cancer effect of midazolam via downregulating YWHAH in papillary thyroid cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Li,Ai-Ping Tan,Yu-Shan Zhong

Kidney & blood pressure research 50:46-60 PubMed39622224

2024

Effects of Cacna1d D307G Mutation on Blood Pressure and Kidney Function in Rats with Salt Loading.

Applications

Unspecified application

Species

Unspecified reactive species

Lan Cheng,Hui Chen,R Nfornah Maboh,Huan Wang

PloS one 19:e0307696 PubMed39038022

2024

Regulation of H9C2 cell hypertrophy by 14-3-3η via inhibiting glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Sha Wan,Songhao Wang,Xianfei Yang,Yalan Cui,Heng Guan,Wenping Xiao,Fang Liu

PloS one 19:e0298820 PubMed38452156

2024

The dispensability of 14-3-3 proteins for the regulation of human cardiac sodium channel Nav1.5.

Applications

Unspecified application

Species

Unspecified reactive species

Oksana Iamshanova,Anne-Flore Hämmerli,Elise Ramaye,Arbresh Seljmani,Daniela Ross-Kaschitza,Noëlia Schärz,Maria Essers,Sabrina Guichard,Jean-Sébastien Rougier,Hugues Abriel

Molecular cancer 22:117 PubMed37481520

2023

Extracellular vesicle-circEHD2 promotes the progression of renal cell carcinoma by activating cancer-associated fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Tao He,Qiansheng Zhang,Peng Xu,Wen Tao,Fuyang Lin,Renfei Liu,Mingzhao Li,Xiaolu Duan,Chao Cai,Di Gu,Guohua Zeng,Yongda Liu

Oncology research 31:615-630 PubMed37415737

2023

YWHAH activates the HMGA1/PI3K/AKT/mTOR signaling pathway by positively regulating Fra-1 to affect the proliferation of gastric cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Junyu He,Feng Zeng,X I Jin,Lin Liang,Mengxiang Gao,Wentao Li,Guiyuan Li,Yanhong Zhou

The Journal of biological chemistry 298:102513 PubMed36150501

2022

Serine 13 of the human cytomegalovirus viral cyclin-dependent kinase UL97 is required for regulatory protein 14-3-3 binding and UL97 stability.

Applications

Unspecified application

Species

Unspecified reactive species

Satoko Iwahori,Angie C Umaña,Robert F Kalejta,Takayuki Murata

International journal of molecular sciences 22: PubMed34884454

2021

Characterization of the Transient Deficiency of PKC Isozyme Levels in Immature Cord Blood T Cells and Its Connection to Anti-Allergic Cytokine Profiles of the Matured Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Khalida Perveen,Alex Quach,Michael J Stark,Susan L Prescott,Simon C Barry,Charles S Hii,Antonio Ferrante

Molecular medicine reports 23: PubMed33495819

2021

Recombinant high‑mobility group box 1 induces cardiomyocyte hypertrophy by regulating the 14‑3‑3η, PI3K and nuclear factor of activated T cells signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Feifei Su,Miaoqian Shi,Jian Zhang,Yan Li,Jianwei Tian

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-14-3-3 eta/YWHAH antibody [EPR16750]

Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (AB206292)

Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (AB206292)

Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (AB206292)

Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (AB206292)

Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (AB206292)

Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (AB206292)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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