AB251443

Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal 1433F antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

YWHA1, YWHAH, 14-3-3 protein eta, Protein AS1

6 Images

Supplier Data

Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (AB251443)

This data was developed using ab206292, the same antibody clone in a different buffer formulation.Negative control : 14-3-3 eta/YWHAH was immunoprecipitated from 1mg of Human brain whole cell lysate with ab206292 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab137106 (which is specific to 14-3-3 gamma) at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : Human brain whole cell lysate 10ug (Input). Lane 2 : ab206292 IP in Human brain whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab206292 in Human brain whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (<a href='/en-us/products/primary-antibodies/14-3-3-eta-ywhah-antibody-epr16750-ab206292'>ab206292</a>)

Predicted band size: 28 kDa

false

Supplier Data

Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (AB251443)

This data was developed using ab206292, the same antibody clone in a different buffer formulation.

14-3-3 eta/YWHAH was immunoprecipitated from 1mg of Human brain whole cell lysate with ab206292 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab206292 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (<a href='/en-us/products/primary-antibodies/14-3-3-eta-ywhah-antibody-epr16750-ab206292'>ab206292</a>)

Predicted band size: 28 kDa

false

Supplier Data

Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (AB251443)

This data was developed using ab206292, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1 : 15 seconds; Lanes 2-3 : 3 minutes.

All lanes:

Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (<a href='/en-us/products/primary-antibodies/14-3-3-eta-ywhah-antibody-epr16750-ab206292'>ab206292</a>) at 1/5000 dilution

Lane 1:

Human fetal brain tissue lysate at 20 µg

Lane 2:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

Lane 3:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Supplier Data

Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (AB251443)

This data was developed using ab206292, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (<a href='/en-us/products/primary-antibodies/14-3-3-eta-ywhah-antibody-epr16750-ab206292'>ab206292</a>) at 1/2000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) cytosolic fraction at 10 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) nuclear fraction at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (AB251443)

This data was developed using ab206292, the same antibody clone in a different buffer formulation. Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (<a href='/en-us/products/primary-antibodies/14-3-3-eta-ywhah-antibody-epr16750-ab206292'>ab206292</a>) at 1000 Da

Lane 1:

14-3-3 beta (YWHAB) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Lane 2:

14-3-3 gamma (YWHAG) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Lane 3:

14-3-3 epsilon (YWHAE) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Lane 4:

14-3-3 zeta (YWHAZ) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Lane 5:

14-3-3 eta (YWHAH) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Lane 6:

14-3-3 theta (YWHAQ) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Lane 7:

14-3-3 sigma (SFN) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Exposure time: 100s

Supplier Data

Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (AB251443)

This data was developed using ab206292, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : Lanes 1-6 : 30 seconds; Lanes 7 -10 : 3 minutes.

All lanes:

Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (<a href='/en-us/products/primary-antibodies/14-3-3-eta-ywhah-antibody-epr16750-ab206292'>ab206292</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Mouse spleen tissue lysate at 10 µg

Lane 3:

Rat brain tissue lysate at 10 µg

Lane 4:

Rat spleen tissue lysate at 10 µg

Lane 5:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

Lane 6:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Lane 7:

Mouse heart tissue lysate at 10 µg

Lane 8:

Mouse kidney tissue lysate at 10 µg

Lane 9:

Rat heart tissue lysate at 10 µg

Lane 10:

Rat kidney tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Unconjugated

Anti-14-3-3 eta/YWHAH antibody [EPR16750]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16750

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab251443 is the carrier-free version of ab206292.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 14-3-3 eta protein also known by its gene name YWHAH functions mechanically as an adapter protein involved in many cellular processes. This protein weighs approximately 28 kDa and facilitates the modulation of signaling pathways by binding to phosphoserine-containing proteins. It exhibits widespread expression with significant presence in the brain emphasizing its role in nervous system activities. The 14-3-3 eta protein acts as a hub in various signal transduction pathways influencing cell cycle regulation and apoptosis.

Biological function summary

The 14-3-3 eta protein interacts with diverse intracellular proteins forming various complexes that influence cellular functions. It plays a pivotal role in cellular stress responses and the regulation of several metabolic processes. The protein modulates activity by altering the localization of target proteins or masking their phosphorylation sites which affects their function and stability. This regulatory capacity emphasizes the protein's impact on cell survival and metabolism.

Pathways

The 14-3-3 eta protein integrates into key signaling networks such as the MAPK/ERK and PI3K/AKT pathways. These pathways are essential in mediating processes like cell proliferation differentiation and survival. Within these pathways 14-3-3 eta functions alongside proteins like RAF1 and AKT1 coordinating cellular responses to external stimuli. Its involvement in these pathways highlights the adaptability and versatile roles it plays in cellular signaling and homeostasis.

The 14-3-3 eta protein has associations with conditions such as rheumatoid arthritis and neurodegenerative diseases. In rheumatoid arthritis altered expression and interactions with other proteins like cytokines contribute to inflammatory processes. In neurodegenerative disorders the dysregulation of 14-3-3 eta affects proteins related to tau pathology influencing disease progression. Understanding these connections helps in comprehending the protein's role in disease mechanisms and developing therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.

See full target information YWHAH

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:26521 PubMed39489850

2024

GABPA inhibits tumorigenesis in clear cell renal cell carcinoma by regulating ferroptosis through ACSL4.

Applications

Unspecified application

Species

Unspecified reactive species

Yaqian Wu,Zonglong Wu,Mengfei Yao,Li Liu,Yimeng Song,Lulin Ma,Cheng Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free

Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (AB251443)

Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (AB251443)

Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (AB251443)

Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (AB251443)

Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (AB251443)

Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (AB251443)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise