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AB252665

Anti-14-3-3 eta/YWHAH antibody [EPR20640-1] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal 1433F antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

YWHA1, YWHAH, 14-3-3 protein eta, Protein AS1

1 Images
Sandwich ELISA - Anti-14-3-3 eta/YWHAH antibody [EPR20640-1] - BSA and Azide free (Detector) (AB252665)
  • sELISA

Lab

Sandwich ELISA - Anti-14-3-3 eta/YWHAH antibody [EPR20640-1] - BSA and Azide free (Detector) (AB252665)

Sandwich ELISA of ab253731 with the capture antibody ab252606 dilution at 2 µg/mL and detector antibody ab252665 dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20640-1

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab252665 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human YWHAH. The recommended pair for sandwich ELISA is:
Capture: ab252606, Human YWHAH Capture Antibody (unconjugated)
Detector: ab252665, Human YWHAH Detector Antibody (unconjugated)
The reference range value is 0.63 - 40 ng/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 14-3-3 eta protein also known by its gene name YWHAH functions mechanically as an adapter protein involved in many cellular processes. This protein weighs approximately 28 kDa and facilitates the modulation of signaling pathways by binding to phosphoserine-containing proteins. It exhibits widespread expression with significant presence in the brain emphasizing its role in nervous system activities. The 14-3-3 eta protein acts as a hub in various signal transduction pathways influencing cell cycle regulation and apoptosis.
Biological function summary

The 14-3-3 eta protein interacts with diverse intracellular proteins forming various complexes that influence cellular functions. It plays a pivotal role in cellular stress responses and the regulation of several metabolic processes. The protein modulates activity by altering the localization of target proteins or masking their phosphorylation sites which affects their function and stability. This regulatory capacity emphasizes the protein's impact on cell survival and metabolism.

Pathways

The 14-3-3 eta protein integrates into key signaling networks such as the MAPK/ERK and PI3K/AKT pathways. These pathways are essential in mediating processes like cell proliferation differentiation and survival. Within these pathways 14-3-3 eta functions alongside proteins like RAF1 and AKT1 coordinating cellular responses to external stimuli. Its involvement in these pathways highlights the adaptability and versatile roles it plays in cellular signaling and homeostasis.

The 14-3-3 eta protein has associations with conditions such as rheumatoid arthritis and neurodegenerative diseases. In rheumatoid arthritis altered expression and interactions with other proteins like cytokines contribute to inflammatory processes. In neurodegenerative disorders the dysregulation of 14-3-3 eta affects proteins related to tau pathology influencing disease progression. Understanding these connections helps in comprehending the protein's role in disease mechanisms and developing therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
See full target information YWHAH
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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