Rabbit Polyclonal 1433G antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human YWHAG aa 1-200.
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Publications
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- iScience 23:1014502020Tunneling Nanotubes Mediate Adaptation of Glioblastoma Cells to Temozolomide and Ionizing Radiation Treatment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSilvana Valdebenito,Alessandra Audia,Krishna P L Bhat,George Okafo,Eliseo A EugeninPubMed 32882515
- Archives of toxicology 94:2861-28722020Functional genetic variants in centrosome-related genes CEP72 and YWHAG confer susceptibility to gastric cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Ni et. al.PubMed 32535685
- Toxicology research 8:875-8842019miR-140-5p mediates bevacizumab-induced cytotoxicity to cardiomyocytes by targeting the /14-3-3γ signal pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuan-Ying Chen et. al.PubMed 32190292
- Heliyon 5:e016672019Identification of the iduronate-2-sulfatase proteome in wild-type mouse brain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarolina Cardona et. al.PubMed 31193135
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human YWHAG aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P61981
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Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Predicted | Predicted | Predicted |
| Chicken | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Monkey | Predicted | Predicted | Predicted |
| Xenopus tropicalis | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Xenopus tropicalis, Rat, Chicken, Cow, Monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Xenopus tropicalis, Rat, Chicken, Cow, Monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Xenopus tropicalis, Rat, Chicken, Cow, Monkey | Dilution info - | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed:15696159, PubMed:16511572, PubMed:36732624). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed:15696159, PubMed:16511572, PubMed:36732624). Binding generally results in the modulation of the activity of the binding partner (PubMed:16511572). Promotes inactivation of WDR24 component of the GATOR2 complex by binding to phosphorylated WDR24 (PubMed:36732624). Participates in the positive regulation of NMDA glutamate receptor activity by promoting the L-glutamate secretion through interaction with BEST1 (PubMed:29121962). Reduces keratinocyte intercellular adhesion, via interacting with PKP1 and sequestering it in the cytoplasm, thereby reducing its incorporation into desmosomes (PubMed:29678907).
Alternative names
14-3-3 protein gamma, Protein kinase C inhibitor protein 1, KCIP-1, YWHAG
Recommended products
Rabbit Polyclonal 1433G antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human YWHAG aa 1-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human YWHAG aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P61981
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
14-3-3 gamma also known as YWHAG or 14-3-3 gamma protein is a member of the 14-3-3 protein family. These proteins play significant roles in signal transduction. 14-3-3 gamma is a dimeric protein with a molecular mass of about 28 to 32 kDa per monomer. It is ubiquitously expressed including in tissues such as brain muscle and heart. This protein influences a wide range of cellular processes by interacting with various signaling proteins often through recognition of phosphorylated serine or threonine motifs.
- Biological function summary
Proteins within the 14-3-3 family like the 14-3-3 gamma function as adaptors and scaffolds in signal transduction pathways. They are part of multi-protein complexes that modulate interactions among proteins within the cell. 14-3-3 gamma interacts dynamically with a variety of target proteins assisting in their activities and proper localization. Its interaction facilitates cellular processes including cell cycle control apoptosis and stress response owing to its activity as a regulator of distinct signaling pathways.
- Pathways
14-3-3 gamma participates in the MAPK/ERK pathway which is pivotal for cell growth and differentiation. It also plays an integral role in the PI3K/AKT pathway which contributes to survival and proliferation signals. In these pathways 14-3-3 gamma interacts with important kinases such as Raf and AKT. These interactions help transduce signals from receptors on the cell surface to the nucleus impacting gene expression and cellular responses.
- Associated diseases and disorders
Research has linked 14-3-3 gamma to neurodegenerative diseases such as Alzheimer's disease. In Alzheimer's 14-3-3 gamma interacts with proteins like tau which become abnormally phosphorylated and aggregate. Additionally this protein has associations with certain cancers where it might influence tumor growth and progression through interactions with cell cycle regulatory proteins. Its modulation of key signaling pathways makes it a candidate of interest in the development of therapeutic strategies for these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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5 product images
Western blot - Anti-14-3-3 gamma/YWHAG antibody (ab155050)
All lanes: Western blot - Anti-14-3-3 gamma/YWHAG antibody (ab155050) at 1/1000 dilution
Lane 1: K562 cell lysate at 30 µg
Lane 2: THP-1 cell lysate at 30 µg
Lane 3: HL-60 cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 28 kDa
Western blot - Anti-14-3-3 gamma/YWHAG antibody (ab155050)
12% SDS PAGE
All lanes: Western blot - Anti-14-3-3 gamma/YWHAG antibody (ab155050) at 1/1000 dilution
Lane 1: 293T whole cell lysate at 30 µg
Lane 2: A431 whole cell lysate at 30 µg
Predicted band size: 28 kDa
Western blot - Anti-14-3-3 gamma/YWHAG antibody (ab155050)
12% SDS PAGE
All lanes: Western blot - Anti-14-3-3 gamma/YWHAG antibody (ab155050) at 1/10000 dilution
All lanes: Mouse brain whole cell lysate at 50 µg
Predicted band size: 28 kDa
Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 gamma/YWHAG antibody (ab155050)
Immunofluorescence analysis of methanol fixed HeLa cells labeling 14-3-3 gamma/YWHAG with ab155050 at 1/200 dilution. Lower image costained with Hoechst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 gamma/YWHAG antibody (ab155050)
Immunohistochemical analysis of paraffin embedded Human DLD1 xenograft tissue labeling 14-3-3 gamma/YWHAG with ab155050 at 1/500 dilution.
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