AB155050

Anti-14-3-3 gamma/YWHAG antibody

Name: Anti-14-3-3 gamma/YWHAG antibody (ab155050) | Abcam
Brand: Abcam Limited
SKU: AB155050
Rating: 4 (1 reviews)

(1 Review)

(5 Publications)

Rabbit Polyclonal 1433G antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human YWHAG aa 1-200.

View Alternative Names

14-3-3 protein gamma, Protein kinase C inhibitor protein 1, KCIP-1, YWHAG

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 gamma/YWHAG antibody (AB155050)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 gamma/YWHAG antibody (AB155050)

Immunohistochemical analysis of paraffin embedded Human DLD1 xenograft tissue labeling 14-3-3 gamma/YWHAG with ab155050 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 gamma/YWHAG antibody (AB155050)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 gamma/YWHAG antibody (AB155050)

Immunofluorescence analysis of methanol fixed HeLa cells labeling 14-3-3 gamma/YWHAG with ab155050 at 1/200 dilution. Lower image costained with Hoechst 33342.

Supplier Data

Western blot - Anti-14-3-3 gamma/YWHAG antibody (AB155050)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-14-3-3 gamma/YWHAG antibody (ab155050) at 1/1000 dilution

Lane 1:

K562 whole cell lysates at 30 µg

Lane 2:

THP-1 whole cell lysates at 30 µg

Lane 3:

HL-60 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 28 kDa

false

Unknown

Western blot - Anti-14-3-3 gamma/YWHAG antibody (AB155050)

12% SDS PAGE

All lanes:

Western blot - Anti-14-3-3 gamma/YWHAG antibody (ab155050) at 1/10000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 28 kDa

false

Supplier Data

Western blot - Anti-14-3-3 gamma/YWHAG antibody (AB155050)

All lanes:

Western blot - Anti-14-3-3 gamma/YWHAG antibody (ab155050) at 1/1000 dilution

Lane 1:

K562 cell lysate at 30 µg

Lane 2:

THP-1 cell lysate at 30 µg

Lane 3:

HL-60 cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human YWHAG aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P61981

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

14-3-3 gamma also known as YWHAG or 14-3-3 gamma protein is a member of the 14-3-3 protein family. These proteins play significant roles in signal transduction. 14-3-3 gamma is a dimeric protein with a molecular mass of about 28 to 32 kDa per monomer. It is ubiquitously expressed including in tissues such as brain muscle and heart. This protein influences a wide range of cellular processes by interacting with various signaling proteins often through recognition of phosphorylated serine or threonine motifs.

Biological function summary

Proteins within the 14-3-3 family like the 14-3-3 gamma function as adaptors and scaffolds in signal transduction pathways. They are part of multi-protein complexes that modulate interactions among proteins within the cell. 14-3-3 gamma interacts dynamically with a variety of target proteins assisting in their activities and proper localization. Its interaction facilitates cellular processes including cell cycle control apoptosis and stress response owing to its activity as a regulator of distinct signaling pathways.

Pathways

14-3-3 gamma participates in the MAPK/ERK pathway which is pivotal for cell growth and differentiation. It also plays an integral role in the PI3K/AKT pathway which contributes to survival and proliferation signals. In these pathways 14-3-3 gamma interacts with important kinases such as Raf and AKT. These interactions help transduce signals from receptors on the cell surface to the nucleus impacting gene expression and cellular responses.

Research has linked 14-3-3 gamma to neurodegenerative diseases such as Alzheimer's disease. In Alzheimer's 14-3-3 gamma interacts with proteins like tau which become abnormally phosphorylated and aggregate. Additionally this protein has associations with certain cancers where it might influence tumor growth and progression through interactions with cell cycle regulatory proteins. Its modulation of key signaling pathways makes it a candidate of interest in the development of therapeutic strategies for these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed : 15696159, PubMed : 16511572, PubMed : 36732624). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed : 15696159, PubMed : 16511572, PubMed : 36732624). Binding generally results in the modulation of the activity of the binding partner (PubMed : 16511572). Promotes inactivation of WDR24 component of the GATOR2 complex by binding to phosphorylated WDR24 (PubMed : 36732624). Participates in the positive regulation of NMDA glutamate receptor activity by promoting the L-glutamate secretion through interaction with BEST1 (PubMed : 29121962). Reduces keratinocyte intercellular adhesion, via interacting with PKP1 and sequestering it in the cytoplasm, thereby reducing its incorporation into desmosomes (PubMed : 29678907).

See full target information YWHAG

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Genes & diseases 12:101519 PubMed40612667

2025

Restriction of YWHAB-mediated YAP cytoplasmic retention is a novel mechanism underlying stemness maintenance and chemoresistance in ovarian cancer peritoneal metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Chang Liu,Lei Shi,Zijun Meng,Manlin Zhang,Zhiqi Zhang,Yunzhe Li,Kaiwen Du,Muyao Yang,Lin Qiu,Jing Feng,Yuchen He,Jiayun Liu,Hua Zhang,Hongbin Zhang,Tingyuan Lang,Zhuo Yang

iScience 23:101450 PubMed32882515

2020

Tunneling Nanotubes Mediate Adaptation of Glioblastoma Cells to Temozolomide and Ionizing Radiation Treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Silvana Valdebenito,Alessandra Audia,Krishna P L Bhat,George Okafo,Eliseo A Eugenin

Archives of toxicology 94:2861-2872 PubMed32535685

2020

Functional genetic variants in centrosome-related genes CEP72 and YWHAG confer susceptibility to gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Ni,Jinchen Wang,Yao Fu,Caiwang Yan,Meng Zhu,Yue Jiang,Jiaping Chen,Yanbing Ding,Xiangshan Fan,Gang Li,Guangfu Jin

Toxicology research 8:875-884 PubMed32190292

2019

miR-140-5p mediates bevacizumab-induced cytotoxicity to cardiomyocytes by targeting the /14-3-3γ signal pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan-Ying Chen,Wei-Lin Huang,Xiao-Ping Peng,Yan-Ni Lv,Jun-He Li,Jian-Ping Xiong

Heliyon 5:e01667 PubMed31193135

2019

Identification of the iduronate-2-sulfatase proteome in wild-type mouse brain.

Applications

Unspecified application

Species

Unspecified reactive species

Carolina Cardona,Eliana Benincore,Natalia Pimentel,Luis H Reyes,Camilo Patarroyo,Alexander Rodríguez-López,M Martin-Rufian,Luis Alejandro Barrera,Carlos J Alméciga-Díaz

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-14-3-3 gamma/YWHAG antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 gamma/YWHAG antibody (AB155050)

Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 gamma/YWHAG antibody (AB155050)

Western blot - Anti-14-3-3 gamma/YWHAG antibody (AB155050)

Western blot - Anti-14-3-3 gamma/YWHAG antibody (AB155050)

Western blot - Anti-14-3-3 gamma/YWHAG antibody (AB155050)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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