AB248786

Anti-14-3-3 gamma/YWHAG antibody [EPR9654(B)] - BSA and Azide free

RabMAb
Recombinant
KO Validated
What is this?

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(1 Publication)

Anti-14-3-3 gamma/YWHAG antibody [EPR9654(B)] - BSA and Azide free (ab248786) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting 14-3-3 gamma/YWHAG in Western Blot. Suitable for Human.

- KO validated for confirmed specificity
- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

14-3-3 protein gamma, Protein kinase C inhibitor protein 1, KCIP-1, YWHAG

2 Images

Lab

Western blot - Anti-14-3-3 gamma/YWHAG antibody [EPR9654(B)] - BSA and Azide free (AB248786)

This data was developed using the same antibody clone in a different buffer formulation (ab137048).

Lanes 1-4 : Merged signal (red and green). Green - ab137048 observed at 28 kDa. Red - loading control ab8245 observed at 36 kDa.

ab137048 Anti-14-3-3 gamma/YWHAG antibody [EPR9654(B)] was shown to specifically react with 14-3-3 gamma/YWHAG in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266734 (knockout cell lysate ab257805) was used. Wild-type and 14-3-3 gamma/YWHAG knockout samples were subjected to SDS-PAGE. ab137048 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-14-3-3 gamma/YWHAG antibody [EPR9654(B)] (<a href='/en-us/products/primary-antibodies/14-3-3-gamma-ywhag-antibody-epr9654b-ab137048'>ab137048</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

YWHAG knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human YWHAG (14-3-3 gamma) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-ywhag-14-3-3-gamma-knockout-hek-293t-cell-line-ab266734'>ab266734</a>)

Lane 3:

A431 cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Unknown

Western blot - Anti-14-3-3 gamma/YWHAG antibody [EPR9654(B)] - BSA and Azide free (AB248786)

This data was developed using ab137048, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-14-3-3 gamma/YWHAG antibody [EPR9654(B)] (<a href='/en-us/products/primary-antibodies/14-3-3-gamma-ywhag-antibody-epr9654b-ab137048'>ab137048</a>) at 1/1000 dilution

Lane 1:

HeLa lysate at 10 µg

Lane 2:

K562 lysate at 10 µg

Lane 3:

A549 lysate at 10 µg

Lane 4:

Human fetal heart lysate at 10 µg

Secondary

All lanes:

HRP conjugated Goat anti Rabbit IgG at 1/2000 dilution

Predicted band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9654(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-14-3-3 gamma/YWHAG antibody [EPR9654(B)] - BSA and Azide free (ab248786) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB) in Human samples.

What is the molecular weight of 14-3-3 gamma/YWHAG?
Anti-14-3-3 gamma/YWHAG [EPR9654(B)] - BSA and Azide free (ab248786) specifically detects a band for 14-3-3 gamma/YWHAG (UniProt: P61981) at a molecular weight of 28kDa.

Specificity confirmed
The specificity of Anti-14-3-3 gamma/YWHAG antibody [EPR9654(B)] - BSA and Azide free (ab248786) has been confirmed by Western blot testing in YWHAG Knockout HEK293T cell line, ab266734.

Other related products
We have a range of other formats of antibody clone [EPR9654(B)] also available for your convenience: ab137048, Carrier free - ab248786

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

14-3-3 gamma also known as YWHAG or 14-3-3 gamma protein is a member of the 14-3-3 protein family. These proteins play significant roles in signal transduction. 14-3-3 gamma is a dimeric protein with a molecular mass of about 28 to 32 kDa per monomer. It is ubiquitously expressed including in tissues such as brain muscle and heart. This protein influences a wide range of cellular processes by interacting with various signaling proteins often through recognition of phosphorylated serine or threonine motifs.

Biological function summary

Proteins within the 14-3-3 family like the 14-3-3 gamma function as adaptors and scaffolds in signal transduction pathways. They are part of multi-protein complexes that modulate interactions among proteins within the cell. 14-3-3 gamma interacts dynamically with a variety of target proteins assisting in their activities and proper localization. Its interaction facilitates cellular processes including cell cycle control apoptosis and stress response owing to its activity as a regulator of distinct signaling pathways.

Pathways

14-3-3 gamma participates in the MAPK/ERK pathway which is pivotal for cell growth and differentiation. It also plays an integral role in the PI3K/AKT pathway which contributes to survival and proliferation signals. In these pathways 14-3-3 gamma interacts with important kinases such as Raf and AKT. These interactions help transduce signals from receptors on the cell surface to the nucleus impacting gene expression and cellular responses.

Research has linked 14-3-3 gamma to neurodegenerative diseases such as Alzheimer's disease. In Alzheimer's 14-3-3 gamma interacts with proteins like tau which become abnormally phosphorylated and aggregate. Additionally this protein has associations with certain cancers where it might influence tumor growth and progression through interactions with cell cycle regulatory proteins. Its modulation of key signaling pathways makes it a candidate of interest in the development of therapeutic strategies for these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed : 15696159, PubMed : 16511572, PubMed : 36732624). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed : 15696159, PubMed : 16511572, PubMed : 36732624). Binding generally results in the modulation of the activity of the binding partner (PubMed : 16511572). Promotes inactivation of WDR24 component of the GATOR2 complex by binding to phosphorylated WDR24 (PubMed : 36732624). Participates in the positive regulation of NMDA glutamate receptor activity by promoting the L-glutamate secretion through interaction with BEST1 (PubMed : 29121962). Reduces keratinocyte intercellular adhesion, via interacting with PKP1 and sequestering it in the cytoplasm, thereby reducing its incorporation into desmosomes (PubMed : 29678907).

See full target information YWHAG

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

NPJ precision oncology 7:95 PubMed37723227

2023

Targeted single-cell proteomic analysis identifies new liquid biopsy biomarkers associated with multiple myeloma.

Applications

Unspecified application

Species

Unspecified reactive species

Sonia M Setayesh,Libere J Ndacayisaba,Kate E Rappard,Valerie Hennes,Luz Yurany Moreno Rueda,Guilin Tang,Pei Lin,Robert Z Orlowski,David E Symer,Elisabet E Manasanch,Stephanie N Shishido,Peter Kuhn

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-14-3-3 gamma/YWHAG antibody [EPR9654(B)] - BSA and Azide free

Western blot - Anti-14-3-3 gamma/YWHAG antibody [EPR9654(B)] - BSA and Azide free (AB248786)

Western blot - Anti-14-3-3 gamma/YWHAG antibody [EPR9654(B)] - BSA and Azide free (AB248786)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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