AB137106

Anti-14-3-3 gamma/YWHAG antibody [EPR9655(B)]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(3 Publications)

Rabbit Recombinant Monoclonal 1433G antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

14-3-3 protein gamma, Protein kinase C inhibitor protein 1, KCIP-1, YWHAG

3 Images

Flow Cytometry (Intracellular) - Anti-14-3-3 gamma/YWHAG antibody [EPR9655(B)] (AB137106)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-14-3-3 gamma/YWHAG antibody [EPR9655(B)] (AB137106)

Intracellular flow cytometric analysis of K562 cells labelling 14-3-3-gamma with ab137106 at 1/10 dilution (red). Negative Rabbit IgG control shown in green.

Unknown

Western blot - Anti-14-3-3 gamma/YWHAG antibody [EPR9655(B)] (AB137106)

All lanes:

Western blot - Anti-14-3-3 gamma/YWHAG antibody [EPR9655(B)] (ab137106) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

A431 cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

K562 cell lysate at 10 µg

Secondary

All lanes:

HRP-labelled Goat anti-Rabbit at 1/2000 dilution

Predicted band size: 28 kDa

false

Supplier Data

Western blot - Anti-14-3-3 gamma/YWHAG antibody [EPR9655(B)] (AB137106)

Blocking and dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-14-3-3 gamma/YWHAG antibody [EPR9655(B)] (ab137106) at 1/1000 dilution

Lane 1:

14-3-3 beta (YWHAB) Human Recombinant Protein 50 ng, with DDDK tagged at 50 ng

Lane 2:

14-3-3 gamma (YWHAG) Human Recombinant Protein 50 ng, with DDDK tagged at 50 ng

Lane 3:

14-3-3 epsilon (YWHAE) Human Recombinant Protein 50 ng, with DDDK tagged at 50 ng

Lane 4:

14-3-3 zeta (YWHAZ) Human Recombinant Protein 50 ng, with DDDK tagged at 50 ng

Lane 5:

14-3-3 eta (YWHAH) Human Recombinant Protein 50 ng, with DDDK tagged at 50 ng

Lane 6:

14-3-3 theta (YWHAQ) Human Recombinant Protein 50 ng, with DDDK tagged at 50 ng

Lane 7:

14-3-3 sigma (SFN) Human Recombinant Protein 50 ng, with DDDK tagged at 50 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Exposure time: 20s

Carrier free

Anti-14-3-3 gamma/YWHAG antibody [EPR9655(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9655(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

14-3-3 gamma also known as YWHAG or 14-3-3 gamma protein is a member of the 14-3-3 protein family. These proteins play significant roles in signal transduction. 14-3-3 gamma is a dimeric protein with a molecular mass of about 28 to 32 kDa per monomer. It is ubiquitously expressed including in tissues such as brain muscle and heart. This protein influences a wide range of cellular processes by interacting with various signaling proteins often through recognition of phosphorylated serine or threonine motifs.

Biological function summary

Proteins within the 14-3-3 family like the 14-3-3 gamma function as adaptors and scaffolds in signal transduction pathways. They are part of multi-protein complexes that modulate interactions among proteins within the cell. 14-3-3 gamma interacts dynamically with a variety of target proteins assisting in their activities and proper localization. Its interaction facilitates cellular processes including cell cycle control apoptosis and stress response owing to its activity as a regulator of distinct signaling pathways.

Pathways

14-3-3 gamma participates in the MAPK/ERK pathway which is pivotal for cell growth and differentiation. It also plays an integral role in the PI3K/AKT pathway which contributes to survival and proliferation signals. In these pathways 14-3-3 gamma interacts with important kinases such as Raf and AKT. These interactions help transduce signals from receptors on the cell surface to the nucleus impacting gene expression and cellular responses.

Research has linked 14-3-3 gamma to neurodegenerative diseases such as Alzheimer's disease. In Alzheimer's 14-3-3 gamma interacts with proteins like tau which become abnormally phosphorylated and aggregate. Additionally this protein has associations with certain cancers where it might influence tumor growth and progression through interactions with cell cycle regulatory proteins. Its modulation of key signaling pathways makes it a candidate of interest in the development of therapeutic strategies for these diseases.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed : 15696159, PubMed : 16511572, PubMed : 36732624). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed : 15696159, PubMed : 16511572, PubMed : 36732624). Binding generally results in the modulation of the activity of the binding partner (PubMed : 16511572). Promotes inactivation of WDR24 component of the GATOR2 complex by binding to phosphorylated WDR24 (PubMed : 36732624). Participates in the positive regulation of NMDA glutamate receptor activity by promoting the L-glutamate secretion through interaction with BEST1 (PubMed : 29121962). Reduces keratinocyte intercellular adhesion, via interacting with PKP1 and sequestering it in the cytoplasm, thereby reducing its incorporation into desmosomes (PubMed : 29678907). Plays a role in mitochondrial protein catabolic process (also named MALM) that promotes the degradation of damaged proteins inside mitochondria (PubMed : 22532927).

See full target information YWHAG

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

FEBS letters 595:2675-2690 PubMed34626438

2021

Disruption of desmosome function leads to increased centrosome clustering in 14-3-3γ-knockout cells with supernumerary centrosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Sarika Tilwani,Karan Gandhi,Satya Narayan,Sri Rama Koti Ainavarapu,Sorab Nariman Dalal

Diagnostics (Basel, Switzerland) 11: PubMed34679529

2021

Is Methionyl-tRNA Synthetase Applicable as a Diagnostic Marker for Lung Cancer in Bronchial Ultrasound-Guided Brushing Cells?

Applications

Unspecified application

Species

Unspecified reactive species

Kyungjong Lee,Mijung Oh,Kyo-Sun Lee,Yoon Jin Cha,Yoon Soo Chang

Genes to cells : devoted to molecular & cellular mechanisms 26:426-446 PubMed33813791

2021

14-3-3γ prevents centrosome duplication by inhibiting NPM1 function.

Applications

Unspecified application

Species

Unspecified reactive species

Arunabha Bose,Kruti Modi,Suchismita Dey,Somavally Dalvi,Prafful Nadkarni,Mukund Sudarshan,Tapas K Kundu,Prasanna Venkatraman,Sorab N Dalal

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-14-3-3 gamma/YWHAG antibody [EPR9655(B)]

Flow Cytometry (Intracellular) - Anti-14-3-3 gamma/YWHAG antibody [EPR9655(B)] (AB137106)

Western blot - Anti-14-3-3 gamma/YWHAG antibody [EPR9655(B)] (AB137106)

Western blot - Anti-14-3-3 gamma/YWHAG antibody [EPR9655(B)] (AB137106)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise